鸡粪好氧堆肥中影响抗生素降解的理化因子和优势菌群分析

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Abstract Taking chicken manure as raw material, the influence of EM microbial agent on the key dominant bacterial groups controlling the degradation of antibiotics were studied in the compost simulated by adding oxytetracycline and tylosin to simulate antibiotic residues. The objective is to explore the bioenhancing mechanism of antibiotic removal in aerobic composting. The experiment was carried out consisting of four treatments, that is, OCK (oxytetracycline), OTK (oxytetracycline +EM bactericide), TCK (Tylomycin + EM bactericide), TTK (Tylomycin + EM bactericide. The amounts of added antibiotics and EM microbial agent were 100mg/kg and 5% of the total mass, respectively. The results showed that the residue of antibiotics had little effect on compost maturation, and all treatments could reach the maturation standard. The composting temperature exceeded 60℃ for more than 6days, the pH value ranged from 8.5 to 9.0, the EC was 3~3.5mS/cm. The removal of both antibiotic mostly happened at high temperature stage, When the removal rates of antibiotics were correspondingly OCK: 71.63%,OTK: 76.40%,TCK: 93.86%,TTK: 92.95%. The EM microbial agent accelerated the degradation of antibiotics, especially for oxytetracycline, which could increase the degradation rate of oxytetracycline by 5 times and that of Tylomycin by 2.24 times. At mature stage, aerobic compost effectively removed the antibiotic residues, with higher antibiotic removal rates of four treatments, OCK 89.97%, OTK 91.32%, TCK 99.99%, TTK 99.99%. pH value is identified as a key physicochemical factor affecting the degradation of oxytetracycline and tylomycin, which was negatively correlated with the content of both antibiotics, so proper adjustment of the pH was conducive to faster degradation of antibiotics. The dominant bacterial groups conducive to oxytetracycline degradation mainly include Planifilum, Thermobifida, Bacillus and Paenibacillus, with Relative abundance (RAs) were 0.24%~8.76%, 2.70%~10.91%, 11.38%~26.16%, and 1.95%~4.56%. While Thermobacillus and Bacillus was helpful for degradation of Tylomycin with RAs were 7.19%~39.09% and 41.70%~44.29%, respectively. Bacillus can promote the degradation of oxytetracycline and tylosin.

Key words： aerobic compost antibiotic biological enhancement dominant bacteriak community

Received: 23 January 2024

PACS:

X705

Corresponding Authors: 田晓美,农艺师,7314115@qq.com;崔彦如,nkycyr@126.com E-mail: 7314115@qq.com;nkycyr@126.com

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	HUANG Fei-fei
	LIU Jian-kun
	WANG Xiao-ming
	LEI Meng-long
	TIAN Xiao-mei
	CUI Yan-ru
	ZHANG Da-lei

Cite this article:

HUANG Fei-fei,LIU Jian-kun,WANG Xiao-ming等. Physicochemical factors and dominant bacterial groups affecting antibiotic degradation in chicken manure aerobic compost[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(8): 4641-4651.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I8/4641

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