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| Influence of sulfide on the desulfurization-denitrification performance of the sulfide-driven autotrophic denitrification and anaerobic ammonia oxidation process |
| WANG Ya-ge1, TANG Xi-fang1, LIU Jia-yi1, FU Yu-lin1, GUO Qiong2, CHEN Rong1, JIN Ren-cun2, XING Bao-shan1,2 |
1. Northwest China Key Laboratory of Water Resource and Environment Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi Province, Key Laboratory of Environmental Engineering, Shaanxi Province, School of Environmental & Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China;
2. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China |
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Abstract In this study, mature anammox granular sludge and methanogenic granular sludge were used as inoculum to achieve rapid start-up of the sulfide-driven autotrophic denitrification and anammox (SADA) process. The effects of different sulfide loading on nitrogen removal of the SADA process and its desulfurization mechanism were investigated. Results showed that high sulfide loads (>1.50g S/(L·d)) had no significant inhibitory effect on Anammox bacteria (AnAOB), and the corresponding nitrogen removal efficiency stabilized after 1.5 months of continuous operation. When the sulfide concentration was reduced to zero, the total nitrogen removal efficiency (TNRE) still reached 88.1%, and the stoichiometric ratios of Rs (1.23 ± 0.13) and Rp (0.33 ± 0.08) were similar to the theoretical values of Anammox reaction, indicating that the AnAOB was capable of performing nitrogen removal after removing the high sulfide loading conditions. When the high sulfide loading stress was relieved for 1.5months and was replaced by lower sulfide loading (0.30g S/(L·d)), the effluent showed a decrease in NO3- with the Rp of 0.21, indicating that the SADA system achieved simultaneous nitrogen and sulfur removal. The presence of methanogenic granular sludge reduced the inhibition of AnAOB caused by the sulfide, which shortened the start-up time of the SADA process. Meanwhile, the coupling system can activate the desulphurization-denitrification process quickly after reintroducing sulfide. Morphological analysis and high-throughput sequencing technique showed that in the SADA system AnAOB (e.g., Candidatus Kuenenia, 16.9%) and sulfur-oxidizing bacteria (e.g.Thiobacillus, 31.6%) were enriched under different sulfide stresses, contributing to high total nitrogen removal (>60%) and sulfur production (95.2%) under high sulfide loading.
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Received: 02 March 2024
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