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| Denitrifying anaerobic methane oxidation microorganisms in shale gas flowback water ― Community structure and key influencing factors |
| LI Xin-yue1,2,3, LU Pei-li1,2,3, LIU Jun2,3, LI Shi-kang2,3, HOU Zheng-ming2, DING A-qiang1,2,3 |
1. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China;
2. College of Environment and Ecology, Chongqing University, Chongqing 400045, China;
3. Key Laboratory of the Three Gorges Reservoir Region's Eco-Environments(Ministry of Education), Chongqing University, Chongqing 400045, China |
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Abstract In this study, we investigated the presence of denitrifying anaerobic methane oxidizing microorganisms in high temperature, high pressure and high salt shale gas flowback water for the first time, along with their correlation with key environmental factors. The results indicated that DAMO archaea and bacteria were detected in all the seven shale gas flowback water, and the archaea exhibiting significantly higher abundance compared to bacteria with 16S rRNA gene copy ranging from 4.32×104～ 5.83×105copies/L and 1.16×103~6.00×103 copies/L, respectively. Additionally, the functional genes mcrA and pomA of DAMO archaea and bacteria were also detected in all the samples. Four representative OTUs of DAMO bacteria and one representative OTU of DAMO archaea were identified in the shale gas flowback water. Despite the high salinity, the DAMO bacteria and archaea detected in flowback water shared a closer genetic relationship to those reported in freshwater environments. RDA correlation analysis revealed that the influence of physicochemical factors in flowback water on DAMO microorganisms was more evident in microbial function rather than microbial abundance. The abundance of pmoA gene was positively and significantly correlated with TOC content, while the abundance of mcrA gene was positively correlated with the concentrations of NO3-, NH4-, PO43-, and Cl-, with Cl- having the greatest impact. These results of the study expanded the understanding of the environmental distribution of DAMO microorganisms, providing a theoretical foundation for controlling greenhouse effect caused by methane emissions in flowback water, and contributing to a deeper comprehension of microbial processes in deep shale formations.
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Received: 19 February 2024
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