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Characteristics of anaerobic ammonium oxidation (anammox) system featuring alternating influent at variable frequencies from top and bottom |
LI Dong1, TAO Bo1, ZHANG Jie1,2 |
1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China; 2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
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Abstract The granulation process of anaerobic ammonia oxidation sludge is slow and easily affected by hydraulic shear force and fluctuation in substrate concentration. At the same time, the mass transfer performance of granular sludge deteriorates with the increase of particle size, which leads to the limitation of nitrogen removal performance of the system. In this paper, a top/bottom variable frequency alternating influent operation mode was proposed, which extends the inlet duration and introduces recirculation to provide sufficient hydraulic shear forces. By varying the inlet positions and frequencies, this method effectively reduces the impact load of substrate concentration on Anammox bacteria and enhances the system's nitrogen removal performance. The changes in sludge concentration, particle performance, EPS(Extracellular Polymeric Substances) secretion and nitrogen removal stability during operation were investigated. The results show that under this influent mode, the removal rate of total nitrogen reached 84.80 %, with an average particle size of 783.67μm, and EPS content of 169.13mg/gVSS. After increasing the influent load, the maximum concentration of free ammonia was 9.70mg/L, which has no inhibitory effect on the system. Microscopic observations revealed darker granule colors and higher heme c content, indicating better AnAOB activity. The high-throughput sequencing results showed that the top/bottom variable frequency alternating influent operation mode can greatly increase the abundance of anaerobic ammonia oxidizing bacteria Candidatus_Brocadia in the system, reaching a peak of 43.59% when the alternating frequency was 3times. The influent mode accelerates the mixing of sludge and substrate, shortens the granulation process, improves sludge activity and resilience to shock loads, and realizes the stable operation of the anaerobic ammonia oxidation system.
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Received: 15 March 2024
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[1] Strous M, Van Gerven E, Kuenen J G, et al. Effects of aerobic and microaerobic conditions on anaerobic ammonium-oxidizing (anammox) sludge [J]. Applied and Environmental Microbiology, 1997,63(6): 2446-2448. [2] 李 航,姚潇涵,张 欣,等.厌氧氨氧化工艺的研究与应用进展 [J]. 工业水处理, 2021,41(2):15-19. Li H, Yao X H, Zhang X, et al. Progress in research and application of anaerobic ammonia oxidation process [J]. industrial water treatment, 2021,41(2):15-19. [3] 桂双林,麦兆环,付嘉琦,等.基于厌氧氨氧化技术的新型生物脱氮工艺研究进展 [J]. 能源研究与管理, 2017,(2):29-33. Gui S L, Mai Z H, Fu J Q, et al. Research progress of novel biological nitrogen removal process based on anaerobic ammonia oxidation technology [J]. Energy Research and Management, 2017,(2):29-33. [4] 王宝玉,朱武卫,付文震,等.厌氧氨氧化工艺在主流污水处理中的应用及其调控研究进展 [J]. 水处理技术, 2024,50(1):20-25. Wang B Y, Zhu W W, Fu W Z, et al. Progress in the application and regulation of anaerobic ammonia oxidation process in mainstream wastewater treatment [J]. Technology of Water Treatment, 2024,50(1):20-25. [5] Xu D, Fan J, Li W, et al. Deciphering correlation between permeability and size of anammox granule: “pores as medium” [J]. Water Research, 2021,191:116832. [6] Li X Y, Yuan Y. Settling velocities and permeabilities of microbial aggregates [J]. Water Research, 2002,36(12):3110-3120. [7] Xu D, Fan J, Pan C, et al. Dimension effect of anammox granule: Potential vs performance [J]. Science of the Total Environment, 2021, 795:148681. [8] Chen W H, Lin J G, Wei Y L, et al. Mass transfer efficiency inside the polyvinyl alcohol-sodium alginate carriers: kinetic changes of immobilized anammox bacteria [J]. Journal of Chemical Technology and Biotechnology, 2020,95(12):3126-3133. [9] Jia F, Yang Q, Liu X, et al. Stratification of Extracellular Polymeric Substances (EPS) for Aggregated Anammox Microorganisms [J]. Environmental Science and Technology, 2017,51(6):3260-3268. [10] Dulekgurgen E, Artan N, Orhon D, et al. How does shear affect aggregation in granular sludge sequencing batch reactors? Relations between shear, hydrophobicity, and extracellular polymeric substances [J]. Water Science and Technology, 2008,58(2):267-276. [11] Kimura Y, Isaka K, Kazama F, et al. Effects of nitrite inhibition on anaerobic ammonium oxidation [J]. Applied Microbiology and Biotechnology, 2010,86(1):359-365. [12] Tang C J, Zheng P, Mahmood Q, et al. Start-up and inhibition analysis of the Anammox process seeded with anaerobic granular sludge [J]. Journal of Industrial Microbiology and Biotechnology, 2009,36(8): 1093-1100. [13] Reino C, Carrera J. Low-strength wastewater treatment in an anammox UASB reactor: Effect of the liquid upflow velocity [J]. Chemical Engineering Journal, 2017,313:217-225. [14] Zhou J H, Ren Q, Xu X L, et al. Enhancing stability of aerobic granules by microbial selection pressure using height-adjustable influent strategy [J]. Water Research, 2021,201:117356. [15] Wu X, Huang J, Lu Z, et al. Thiothrix eikelboomii interferes oxygen transfer in activated sludge [J]. Water Research, 2019,151:134-143. [16] Luo J, Zhang Q, Zhao J, et al. Potential influences of exogenous pollutants occurred in waste activated sludge on anaerobic digestion: A review [J]. Journal of Hazardous Materials, 2020,383:121176. [17] Wan C, Yang X, Lee D J, et al. Formation of filamentous aerobic granules: role of pH and mechanism [J]. Applied Microbiology and Biotechnology, 2014,98(19):8389-8397. [18] Jaroszynski L W, Cicek N, Sparling R, et al. Importance of the operating pH in maintaining the stability of anoxic ammonium oxidation (anammox) activity in moving bed biofilm reactors [J]. Bioresource Technology, 2011,102(14):7051-7056. [19] 邵 磊,伍昌年,薛莉娉,等.关于厌氧氨氧化基质底物抑制的研究进展 [J]. 西安文理学院学报(自然科学版), 2023,26(2):68-73. Shao L, Wu C N, Xue L P, et al. Research progress on substrate inhibition of anaerobic ammonia oxidation [J]. Journal of Xi'an University (Natural Science Edition), 2023,26(2):68-73. [20] Li X, Du R, Zhang J, et al. Deciphering the spatial distribution along the upflow anammox reactor: Sludge characteristics and interspecies interactions [J]. Bioresource Technology, 2022,361:127748. [21] Lu H F, Zheng P, Ji Q X, et al. The structure, density and settlability of anammox granular sludge in high-rate reactors [J]. Bioresource Technology, 2012,123:312-317. [22] Van De Graaf A A, De Bruijn P, Robertson L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor [J]. Microbiology, 1996,142(8):2187-2196. [23] Fr Olund B G T, Nielsen P H. Enzymatic activity in the activated-sludge floc matrix [J]. Applied Microbiology and Biotechnology, 1995, 43(4):755-761. [24] A A. Standard methods for the examination of water and wastewater [M].American: American Public Health Association, 1998. [25] 王晓曈,杨 宏.基于粒径分化的厌氧氨氧化污泥性能与微生物多样性分析 [J]. 环境科学, 2021,42(4):1930-1938. Wang X T, Yang H. Analysis of performance and microbial diversity of anaerobic ammonium oxidation sludge based on particle size differentiation [J]. Environmental Science, 2021,42(4):1930-1938. [26] Waki M, Tokutomi T, Yokoyama H, et al. Nitrogen removal from animal waste treatment water by anammox enrichment [J]. Bioresource Technology, 2007,98(14):2775-2780. [27] Fernández I, Dosta J, Fajardo C, et al. Short-and long-term effects of ammonium and nitrite on the Anammox process [J]. Journal of Environmental Management, 2012,95:S170-S174. [28] Samperio-Ramos G, Hernández-Sánchez O, Camacho-Ibar V F, et al. Ammonium loss microbiologically mediated by Fe(III) and Mn(IV) reduction along a coastal lagoon system [J]. Chemosphere, 2024,349:140933. [29] Hou X, Liu S, Zhang Z. Role of extracellular polymeric substance in determining the high aggregation ability ofanammox sludge [J]. Water Research, 2015,75:51-62. [30] 王雪萍.厌氧氨氧化污泥颗粒化的控制策略及饥饿胁迫下细胞凋亡特性 [D]. 青岛:青岛大学, 2022. Wang X P. Control strategy of anaerobic ammonia-oxidized sludge granulation and apoptosis characteristics under starvation stress [D]. Qingdao: Qingdao University, 2022. [31] Chen J, Ji Q, Zheng P, et al. Floatation and control of granular sludge in a high-rate anammox reactor [J]. Water Research, 2010,44(11): 3321-3328. [32] Ni S Q, Yang N. Evaluation of granular anaerobic ammonium oxidation process for the disposal of pre-treated swine manure [J]. PeerJ, 2014,2014(1):336. [33] 陈方敏,顾澄伟,胡羽婷,等.厌氧氨氧化污泥恢复过程中的颗粒特性 [J]. 环境科学, 2018,39(12):5605-5611. Chen F M, Gu C W, Hu Y T, et al. Particle characteristics of anaerobic ammonium oxidation sludge during recovery process [J]. Environmental Science, 2018,39(12):5605-5611. [34] Chen W, Hu F, Li X, et al. Deciphering the mechanism of medium size anammox granular sludge driving better nitrogen removal performance [J]. Bioresource Technology, 2021,336:125317. [35] Zhu G, Wang S, Ma B, et al. Anammox granular sludge in low-ammonium sewage treatment: Not bigger size driving better performance [J]. Water Research, 2018,142:147-158. [36] Liu Y Q, Tay J H. Characteristics and stability of aerobic granules cultivated with different starvation time [J]. Applied Microbiology and Biotechnology, 2007,75(1):205-210. [37] 康 达,郑 平,胡倩怡.厌氧氨氧化结构体、形态与功能 [J]. 化工学报, 2016,67(10):4040-4046. Kang D, Zheng P, Hu Q Y. Structure, morphology and function of anaerobic ammonia oxidation [J]. CIESC journal, 2016,67(10):4040-4046. [38] Ye L, Li D, Zhang J, et al. Resuscitation of starved anaerobic ammonium oxidation sludge system: Impacts of repeated short-term starvation [J]. Bioresource Technology, 2018,263:458-466. [39] Mu Y, Yu H Q, Wang G. Permeabilities of anaerobic CH4-producing granules [J]. Water Research, 2006,40(9):1811-1815. [40] Mal N K, Fujiwara M, Tanaka Y. Photocontrolled reversible release of guest molecules from coumarin-modified mesoporous silica [J]. Nature, 2003,421(6921):350-353. [41] He S, Feng L, Zhao W, et al. Composition and molecular structure analysis of hydrophilic/hydrophobic extracellular polymeric substances (EPS) with impacts on sludge dewaterability [J]. Chemical Engineering Journal, 2023,462:142234. [42] Yang D, Jiang C, Xu S, et al. Insight into nitrogen removal performance of anaerobic ammonia oxidation in two reactors: Comparison based on the aspects of extracellular polymeric substances and microbial community [J]. Biochemical Engineering Journal, 2022,185:108526. [43] 陈重军,曹茜斐,邹馨怡,等.厌氧氨氧化颗粒污泥EPS的作用、成分及影响因素研究进展 [J]. 环境工程学报, 2022,16(2):381-389. Chen C J, Cao Q F, Zou X Y, et al. Research progress on the role, composition and influencing factors of EPS in anaerobic ammonia-oxidized granular sludge [J]. Chinese Journal of Environmental Engineering, 2022,16(2):381-389. [44] Zhao Y, Feng Y, Li J, et al. Insight into the Aggregation Capacity of Anammox Consortia during Reactor Start-Up [J]. Environmental Science & Technology, 2018,52(6):3685-3695. [45] Jung J Y, Kang S H, Chung Y C, et al. Factors affecting the activity of anammox bacteria during start up in the continuous culture reactor [J]. Water Science and Technology, 2007,55(1-2):459-468. |
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