除草剂对质粒介导ARGs接合转移的影响及机制

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (3026 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract The spread of antibiotic resistance genes (ARGs) in farmland ecosystem is directly related to human health, and has attracted much attention in recent years. Herbicides are common chemical reagents widely used in farmland ecosystem, and until now, little information has been known about its effects and mechanisms on horizontal gene transfer (HGT) of ARGs between bacteria. In this study, with Escherichia coli DH5α(carrying RP4 plasmid with Tet^R, Amp^R, Kan^R) as the donor and E. coli HB101as the recipient, a series of diparental conjugation experiments were conducted to investigate the effects of butachlor, paracetamol and glufosinate on HGT of ARGs via plasmid-mediated conjugation. Furthermore, the mechanisms involved were also clarified from cell membrane permeability, intracellular ROS content, and the transcription levels of genes related to conjugation. The results showed that 100~800mg/L of butachlor and 40~160mg/L of paracetamol could promote the conjugation transfer of plasmid RP4. Under test concentrations, butachlor stimulated the intracellular production of ROS, while paraquat reduced the ROS level. Under the treatment of butachlor or paracetamol, the surface of the recipient bacterium appeared obvious shrinkage, and with the increase of concentration, the pore and even break appeared. Butachlor (400~800mg/L) and paraquat (40~80mg/L) could both increase cell membrane permeability, inhibit the korA transcription and promote the transcription of trbBp、trfAp and traA, thus promoting the conjugation transfer of RP4. Under test concentrations, there was only a little effect of glufosinate on RP4 conjugation, as well as cell membrane permeability, intracellular ROS content and transcription of conjugation-related regulatory genes. This study indicated that widespread use of herbicides in agricultural production could affect the HGT of ARGs via plasmid-mediated conjugation, and the application of reasonable types and concentrations of herbicides could reduce the ARG transmission in both soil environments and plants.

Key words： herbicides mobile plasmid RP4 antibiotic resistance genes horizontal transfer conjugation

Received: 05 May 2024

PACS:

X53

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	SUN Feng-fei
	WANG Xiu
	MAO Yu-meng
	LIU Ke
	LIU Juan

Cite this article:

SUN Feng-fei,WANG Xiu,MAO Yu-meng等. Effects and mechanisms of herbicides on horizontal transfer of antibiotic resistance genes through plasmid-mediated conjugation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6426-6434.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I11/6426

[1] Larranaga O, Brown-Jaque M, Quiros P, et al. Phage particles harboring antibiotic resistance genes in fresh-cut vegetables and agricultural soil [J]. Environment International, 2018,115:133-141.
[2] Chen Q L, An X L, Zheng B X, et al. Long-term organic fertilization increased antibiotic resistome in phyllosphere of maize [J]. Science of the Total Environment, 2018,645:1230-1237.
[3] Soucy S M, Huang J L, Gogarten J P. Horizontal gene transfer: building the web of life [J]. Nature Reviews Genetics, 2015,8:472-482.
[4] Martinez J L. Environmental pollution by antibiotics and by antibiotic resistance determinants [J]. Environmental Pollution, 2009,157:2893-2902.
[5] Founou L L, Founou R C, and Essack S Y. Antibiotic resistance in the food chain: a developing country-perspective [J]. Frontiers in Microbiology, 2016,7:1881.
[6] Virolle C, Goldlust K, Djermoun S, et al. Plasmid transfer by conjugation in gram-negative bacteria: from the cellular to the community level [J]. Genes, 2020,11:1239.
[7] Meftaul I M, Venkateswarlu K, Dharmarajan R, et al. Controversies over human health and ecological impacts of glyphosate: Is it to be banned in modern agriculture [J]. Environmental Pollution, 2020,263: 114372.
[8] Dayan F E. Current status and future prospects in herbicide discovery [J]. Plants (Basel), 2019,8(9):341.
[9] 石晓旭,陈亦,黄嵘.我国化学除草剂使用现状及对策 [J]. 现代农业科技, 2016,19:133-134. Shi X X, Chen Y, Huang R. Current situation and countermeasures of chemical herbicides in our country [J]. Modern Agricultural Technology, 2016,19:133-134.
[10] Kurenbach B, Gibson P S, Hill A M, et al. Herbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responses [J]. Microbiology, 2017,163:1791-1801.
[11] Kurenbach B, Marjoshi D, Amabile-Cuevas C F, et al. Sublethal exposure to commercial formulations of the herbicides dicamba, 2,4-dichlorophenoxyacetic acid, and glyphosate cause changes in antibiotic susceptibility in Escherichia coli and Salmonella enterica serovar Typhimurium [J]. mBio., 2015,6(2):e00009-15.
[12] Liao H, Li X, Yang Q, et al. Herbicide selection promotes antibiotic resistance in soil microbiomes [J]. Molecular Biology and Evolution, 2021,38:2337-2350.
[13] Zhang H, Liu J, Wang L, et al. Glyphosate escalates horizontal transfer of conjugative plasmid harboring antibiotic resistance genes [J]. Bioengineered, 2020,12(1):63-69.
[14] Li X, Wen C, Liu C, et al. Herbicide promotes the conjugative transfer of multi-resistance genes by facilitating cellular contact and plasmid transfer [J]. Journal of Environmental Sciences, 2022,115:363-373.
[15] 张厚朴.农用杀菌剂胁迫下质粒RP4介导的抗生素抗性基因接合转移及其机制 [D]. 杭州:浙江大学农业与生物技术学院, 2021. Zhang H P. Conjugative transfer and its mechanism of antibiotic resistance genes mediated by plasmid RP4under agricultural fungicide stress [D]. Hangzhou: College of Agriculture & Biotechnology, Zhejiang University, 2021.
[16] Liu X M, Tang J C, Song B R, et al. Exposure to Al₂O₃ nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces [J]. Nanotoxicology, 2019,13:1422-1436.
[17] Jiao Y, Niu L N, Ma S, et al. Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance [J]. Progress in Polymer Science, 2017,71:53-90.
[18] Zhao H, Liu X, Sun Y, et al. Effects and mechanisms of plant growth regulators on horizontal transfer of antibiotic resistance genes through plasmid-mediated conjugation [J]. Chemosphere, 2023,318:137997.
[19] He K, Xue B, Yang X B, et al. Low-concentration of trichloromethane and dichloroacetonitrile promote the plasmid-mediated horizontal transfer of antibiotic resistance genes [J]. Journal of Hazardous Materials, 2022,425(e):128030.
[20] Chen X F, Yin H L, Li G Y, et al. Antibiotic-resistance gene transfer in antibiotic-resistance bacteria under different light irradiation: Implications from oxidative stress and gene expression [J]. Water Research, 2018,149:282-291.
[21] Xie S S, Gu A Z, Cen T Y, et al. The effect and mechanism of urban fine particulate matter (PM_2.5) on horizontal transfer of plasmid-mediated antimicrobial resistance genes [J]. Science of the Total Environment, 2019,683:116-123.
[22] Wang Y, Lu J, Mao L K, et al. Antiepileptic drug carbamazepine promotes horizontal transfer of plasmid-borne multi-antibiotic resistance genes within and across bacterial genera [J]. The ISME Journal, 2019,13:509-522.
[23] Wang Q, Mao D Q, and Luo Y. Ionic liquid facilitates the conjugative transfer of antibiotic resistance genes mediated by plasmid RP4 [J]. Environmental Science & Technology, 2015,49:8731-8740.
[24] Qiu Z G, Yu Y M, Chen Z L, et al. Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera [J]. Proceedings of the National Academy of Sciences of the United Statesof America, 2012,109:4944-4949.
[25] Tyohemba R L, Humphrie M S, Schleyer M H, et al. Accumulation of commonly used agricultural herbicides in coral reef organisms from iSimangaliso Wetland Park, South Africa [J]. Environmental Pollution, 2022,294:118665.
[26] Ouyang W, Zhang Y, Gu X, et al. Occurrence, transportation, and distribution difference of typical herbicides from estuary to bay [J]. Environment International, 2019,130:104858.
[27] Hertel R, Gibhardt J, Martienssen M, et al. Molecular mechanisms underlying glyphosate resistance in bacteria [J]. Environmental Microbiology, 2021,23(6):2891-2905.
[28] Li Z Q, Gao J F, Guo Y, et al. Enhancement of antibiotic resistance dissemination by artificial sweetener acesulfame potassium:Insights from cell membrane, enzyme, energy supply and transcriptomics [J]. Journal of Hazardous Materials, 2022,422:126942.
[29] Xiong R, Liu Y Y, Pu J Y, et al. Indole inhibits IncP-1conjugation system mainly through promoting korA and korB expression [J]. Frontiers in Microbiology, 2021,12:628133.
[30] Huang H, Chen Y, Yang S, et al. CuO and ZnO nanoparticles drive the propagation of antibiotic resistance genes during sludge anaerobic digestion: possible role of stimulated signal transduction [J]. Environmental Science-Nano, 2018,6(2):528-539.
[31] Feehily C, Karatzas K. Role of glutamate metabolism in bacterial responses towards acid and other stresses [J]. Journal of Applied Microbiology, 2013,114(1):11-24.
[32] Wu S, Ren P F, Wu Y C, et al. Effects of hematite on the dissemination of antibiotic resistance in pathogens and underlying mechanisms [J]. Journal of Hazardous Materials, 2022,431:128537.
[33] Zhu B K, Chen Q L, Chen S C, et al. Effect and mechanism of quorum sensing on horizontal transfer of multidrug plasmid RP4 in BAC biofilm [J]. Science of The Total Environment, 2020,98:152-159.
[34] Guo A Y, Zhou Q X, Bao Y Y, et al. Prochloraz alone or in combination with nano-CuO promotes the conjugative transfer of antibiotic resistance genes between Escherichia coli in pure water [J]. Journal of Hazardous Materials, 2022,424:127761.
[35] Lu J, Wang Y, Jin M, et al. Both silver ions and silver nanoparticles facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes [J]. Water Research, 2020,169:115229.