|
|
|
| Correlation of nitrogen metabolism and heavy metal resistance genes in anammox sludges |
| QU Cai-yan, TANG Xi, CHENG Si-yuan, GUAN Hui-ze, TANG Chong-jian |
| National Engineering Research Center for Control and Treatment of Heavy Metal Pollution, School of Metallurgy and Environment, Central South University, Changsha 410083, China |
|
|
|
|
Abstract To elucidate the interaction between nitrogen metabolism and heavy metal resistance in different sludge aggregates, we investigated gene abundance and correlations of nitrogen metabolism genes and heavy metal resistance genes. The gene abundance analysis revealed that the total abundance of nitrogen metabolism genes and heavy metal resistance genes in granules was higher than in flocs (by 24.5% and 5.5%, respectively) and biofilm (by 57.9% and 16.7%, respectively), indicating that granules exhibit the highest potential for nitrogen metabolism and heavy metal resistance. Further, the network analysis showed that the average degree of the granule network was 63.0% and 54.4% higher than in floc network and biofilm network, respectively, highlighting the strongest correlation between nitrogen metabolism genes and heavy metal resistance genes in granules. Therefore, the granule is the optimal sludge aggregate for anammox-based process in treating ammonia-rich wastewater containing heavy metals.
|
|
Received: 28 May 2024
|
|
|
|
|
|
[1] Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences-an application survey [J]. Water Research, 2014,55:292-303.
[2] Tang X, Guo Y, Wu S, et al. Metabolomics uncovers the regulatory pathway of acyl-homoserine lactones based quorum sensing in anammox consortia [J]. Environmental Science & Technology, 2018, 52(4):2206-2216.
[3] Jin R C, Yang G F, Yu J J, et al. The inhibition of the Anammox process: a review [J]. Chemical Engineering Journal, 2012,197:67-79.
[4] 韩雪恪,王峥嵘,彭永臻,等.几种重要因素对厌氧氨氧化过程的影响综述[J]. 中国环境科学, 2023,43(5):2220-2227. Han X K, Wang Z R, Peng Y Z, et al. A review of several important factors influencing the Anammox process [J]. China Environmental Science, 2023,43(5):2220-2227.
[5] Song Y X, Chai L Y, Tang C J, et al. Influence of ZnO nanoparticles on anammox granules: The inhibition kinetics and mechanism analysis by batch assays [J]. Biochemical Engineering Journal, 2018,133:122-129.
[6] Lotti T, Cordola M, Kleerebezem R, et al. Inhibition effect of swine wastewater heavy metals and antibiotics on anammox activity [J]. Water Science and Technology, 2012,66(7):1519-1526.
[7] 李智卓,姚福兵,吴星,等.硝酸盐废水电化学选择性还原产氨的研究进展[J]. 能源环境保护, 2023,37(4):56-67. Li Z Z, Yao F B, Wu X, et al. Progress in selective electronchemical reduction of nitrate into ammonia [J]. Energy Environmental Protection, 2023,37(4):56-67.
[8] Li G, Puyol D, Carvajal‐Arroyo J M, et al. Inhibition of anaerobic ammonium oxidation by heavy metals [J]. Journal of Chemical Technology & Biotechnology, 2015,90(5):830-837.
[9] Qu C, Li L, Feng F, et al. Enhancement of extracellular Cr(VI) reduction for anammox recovery using hydrazine: performance, pathways, and mechanism [J]. Frontiers of Environmental Science & Engineering, 2023,17(9):115.
[10] Qu C, Duan C, Li W, et al. Understanding the slight inhibition of high As (III) stress on nitritation process: Insights from arsenic speciation and microbial community analyses [J]. Journal of Hazardous Materials, 2022,435:128957.
[11] Jiang Y, Zhang B, He C, et al. Synchronous microbial vanadium (V) reduction and denitrification in groundwater using hydrogen as the sole electron donor [J]. Water Research, 2018,141:289-296.
[12] Harrison J J, Ceri H, Turner R J. Multimetal resistance and tolerance in microbial biofilms [J]. Nature Reviews Microbiology, 2007,5(12):928.
[13] Bruins M R, Kapil S, Oehme F W. Microbial resistance to metals in the environment [J]. Ecotoxicology and Environmental Safety, 2000, 45(3):198-207.
[14] Qu C, Feng F, Tang J, et al. A review of quorum sensing regulating heavy metal resistance in anammox process: Relations, mechanisms and prospects [J]. Critical Reviews in Environmental Science and Technology, 2024,54(5):369-384.
[15] Daraei H, Rafiee M, Yazdanbakhsh A R, et al. A comparative study on the toxicity of nano zero valent iron (nZVI) on aerobic granular sludge and flocculent activated sludge: Reactor performance, microbial behavior, and mechanism of toxicity [J]. Process Safety and Environmental Protection, 2019,129:238-248.
[16] Jiang Y, Liu Y, Zhang H, et al. Aerobic granular sludge shows enhanced resistances to the long-term toxicity of Cu(II) [J]. Chemosphere, 2020,253:126664.
[17] Buchfink B, Xie C, Huson D H. Fast and sensitive protein alignment using DIAMOND [J]. Nature Methods, 2015,12(1):59-60.
[18] Ighodaro O M, Akinloye O A. First line defence antioxidantssuperoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid [J]. Alexandria Journal of Medicine, 2018,54(4):287-293.
[19] Li J, Peng Y, Zhang L, et al. Quantify the contribution of anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor [J]. Water Research, 2019,160:178-187.
[20] Speth D R, in’t Zandt M H, Guerrero-Cruz S, et al. Genome-based microbial ecology of anammox granules in a full-scale wastewater treatment system [J]. Nature Communications, 2016,7(1):11172.
[21] 常尧枫,郭萌蕾,谢军祥,等.厌氧氨氧化脱氮除碳功能菌群结构及代谢途径[J]. 中国环境科学, 2022,42(3):1138-1145. Chang Y F, Guo M L, Xie J X, et al.The structure and metabolic pathway of functional bacteria for nitrogen and carbon removal in Anammox [J]. China Environmental Science, 2022,42(3):1138-1145.
[22] Tang X, Guo Y, Jiang B, et al. Metagenomic approaches to understanding bacterial communication during the anammox reactor start-up [J]. Water Research, 2018,136:95-103.
[23] Wang T, Zhang H, Gao D, et al. Comparison between MBR and SBR on Anammox start-up process from the conventional activated sludge [J]. Bioresource Technology, 2012,122:78-82.
[24] Zhao Y, Jiang B, Tang X, et al. Metagenomic insights into functional traits variation and coupling effects on the anammox community during reactor start-up [J]. Science of the Total Environment, 2019, 687:50-60.
[25] 贾方旭,彭永臻,王衫允,等.厌氧氨氧化菌细胞的超微结构及功能[J]. 应用与环境生物学报, 2014,20(5):944-954. Jia F X, Peng Y Z, Wang S Y, et al. Ultrastructure and function of anaerobic ammonium oxidation bacteria cells [J]. Chinese Journal of Applied and Environmental Biology, 2014,20(5):944-954.
[26] Kuenen J G. Anammox bacteria: from discovery to application [J]. Nature Reviews Microbiology, 2008,6(4):320-326.
[27] 罗镕, 李军, 高鹏, 等. 冬季 自然 温度 下外 加AHLs对ANAMMOX生物膜的影响[J]. 中国环境科学, 2024,44(8):4292- 4302. Luo R, Li J, Gao P, et al. Effect of exogenous AHLs on ANAMMOX biofilm process under natural temperature in winter [J]. China Environmental Science, 2024,44(8):4292-4302.
[28] Feng F, Liu Z, Tang X, et al. Dosing with pyrite significantly increases anammox performance: Its role in the electron transfer enhancement and the functions of the Fe-N-S cycle [J]. Water Research, 2023,229: 119393.
[29] Hall-Stoodley L, Costerton J W, Stoodley P. Bacterial biofilms: from the natural environment to infectious diseases [J]. Nature Reviews Microbiology, 2004,2(2):95-108.
[30] Guo J, Peng Y, Fan L, et al. Metagenomic analysis of anammox communities in three different microbial aggregates [J]. Environmental Microbiology, 2016,18(9):2979-2993.
[31] Zhao Y, Liu S, Jiang B, et al. Genome-centered metagenomics analysis reveals the symbiotic organisms possessing ability to crossfeed with anammox bacteria in anammox consortia [J]. Environmental Science & Technology, 2018,52(19):11285-11296.
[32] Gao D, Liu L, Liang H, et al. Aerobic granular sludge: characterization, mechanism of granulation and application to wastewater treatment [J]. Critical Reviews in Biotechnology, 2011,31(2):137-152.
[33] 赵一淳,刘亚雷,李亦舒,等.强污泥流失对anammox工艺脱氮性能及菌群的影响[J]. 中国环境科学, 2022,42(5):2161-2168. Zhao Y C, Liu Y L, Li Y S, et al. Effects of high sludge loss on the nitrogen removal performance and microbial community of anammox process [J]. China Environmental Science, 2022,42(5):2161-2168.
[34] 王倩,胡嘉源,李天皓,等.铁强化厌氧氨氧化脱氮机理研究进展[J]. 中国环境科学, 2022,42(11):5153-5162. Wang Q, Hu J Y, Li Y S, et al. Research progress on the mechanisms of iron-assisted anaerobic ammonia oxidation process. [J]. China Environmental Science, 2022,42(11):5153-5162.
[35] Hemme C L, Deng Y, Gentry T J, et al. Metagenomic insights into evolution of a heavy metal-contaminated groundwater microbial community [J]. The ISME Journal, 2010,4(5):660-672.
[36] Silver S, Phung L T. Bacterial heavy metal resistance: new surprises [J]. Annual Review of Microbiology, 1996,50(1):753-789.
[37] Viti C, Marchi E, Decorosi F, et al. Molecular mechanisms of Cr(VI) resistance in bacteria and fungi [J]. FEMS Microbiology Reviews, 2014,38(4):633-659.
[38] Thatoi H, Das S, Mishra J, et al. Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: A review [J]. Journal of Environmental Management, 2014,146:383- 399.
[39] Yu C, Tang X, Li L S, et al. The long-term effects of hexavalent chromium on anaerobic ammonium oxidation process: Performance inhibition, hexavalent chromium reduction and unexpected nitrite oxidation [J]. Bioresource Technology, 2019,283:138-147.
[40] Yuan S, Xu R, Wang D, et al. Ecological linkages between a biofilm ecosystem and reactor performance: the specificity of biofilm development phases [J]. Environmental Science & Technology, 2021, 55(17):11948-11960.
[41] Barathi S, Meng Y, Yu Z, et al. Roles of nitrite in mediating the composition and metacommunity of multispecies biofilms [J]. Journal of Water Process Engineering, 2021,40:101764. |
|
|
|
