土地利用对黄河上游河流碳氮循环微生物的影响

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Abstract In this study, the Qingshui River, a typical river in arid and semi-arid areas of the upper reaches of the Yellow River, was investigated. The metagenomic technology was used to reveal the impact of land use on carbon and nitrogen cycling microorganisms in the water bodies. The findings revealed the water quality was less polluted in forest reaches, the average concentrations of NH₄⁺-N, NO₃^--N, TN, and COD were 0.19, 1.35, 1.66, and 21.92mg/L, respectively. Conversely, the NH₄⁺-N(4.23mg/L) and COD(48.8mg/L) concentration elevated in the water body in urban and rural reaches, while NO₃^--N(21.62mg/L) and TN(31.59mg/L) contents increased in agricultural reaches. The relative abundance of the nitrification genes amoAB, nxrAB and the carbon cycling genes sucCD, IDH13, mdh, porAB, and gap2 was higher in forested reaches, while the relative abundance of the denitrification genes napAB, nirKS, and nosZ was higher in urban and rural area reaches, and in agricultural area reaches. The dominant microbial species of carbon and nitrogen functional were higher in forested reaches and urban and rural reaches with 20 species than in agricultural reaches with 15species. The microbial network showed that the number of correlation edges was less than 35 in urban and rural reaches and agricultural reaches, and more than 35 in forested reaches, thus the microbial network structures were notably more complex in forested reaches. In urban and rural reaches, Paracoccus and Sphingomonas were significantly negatively correlated with NH₄⁺-N concentration, while Thalassiosira were significantly positively correlated with NO₃^--N concentration. In agricultural reaches, Leptothrix and Methylocystis were significantly negatively correlated with NH₄⁺-N and COD contents; while Acidovorax, Flavobacterium, and Hydrogenophaga were significantly negatively correlated with NO₃^--N concentration.

Key words： upper reaches of the Yellow River river land use carbon-nitrogen cycle microorganisms

Received: 30 May 2024

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X522

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	Articles by authors
	YE Ling
	HUANG Jing-jie
	YIN Qiong-lin
	XIE Lei
	BAI Xue
	PANG Qing-qing
	ZHAO Ju
	YANG Wei-gong
	PENG Fu-quan
	ZHU Xiang
	YANG Fei
	WANG Long-mian

Cite this article:

YE Ling,HUANG Jing-jie,YIN Qiong-lin等. The effects of land use patterns on carbon and nitrogen cycling microbe in a river of the upper reaches of the Yellow River[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(12): 6846-6857.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I12/6846

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