PE和PLA微塑料对水体氮代谢相关功能微生物的影响

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (4320 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract To have explored the mechanism of the impact of MPs on the nitrogen metabolism function of water bodies, the study had conducted indoor simulation experiments from the perspective of microorganisms. Specifically, it had experimentally tested the impact of traditional polyethylene (PE) microplastics and biodegradable polylactic acid (PLA) microplastics at various concentrations (0, 1, 5, and 10mg/L) on total nitrogen (TN), ammonium (NH₄⁺-N), nitrite (NO₂^--N), and nitrate (NO₃^--N) levels. Furthermore, it had analyzed the effects on nitrogen-metabolizing microbial communities and their functional genes. The results had shown that both PE and PLA microplastics had contribute to nitrogen accumulation in water. PE microplastics increased TN concentrations by 53.77% to 94.76%, while PLA microplastics had caused an increase of 24.04% to 48.74% compared to the control. The impact on different nitrogen forms had varied according to the type and concentration of microplastics. Notably, PE microplastics had been negatively correlated with nitrogen-fixing bacteria, such as Cyanobacteria, whereas PLA microplastics had exhibited a positive correlation with bacteria involved in inorganic nitrogen processes, such as Actinobacteria. It had further shown that both PE and PLA microplastics had significantly affected genes responsible for nitrogen fixation, nitrate reduction, and denitrification. This research had highlightsed the complex effects of microplastics on nitrogen cycling in aquatic systems, with the same particle size but different types and concentrations of MPs leading to varied outcomes on microbial community structure and nitrogen metabolism functions.

Key words： microplastics (MPs) water bodies nitrogen metabolism microorganisms functional genes

Received: 19 June 2024

PACS:

X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WANG Zhi-chao
	MA Yu
	YANG Li-wen
	YIN Zhen-yu
	BAI Long
	LI Wei-ping

Cite this article:

WANG Zhi-chao,MA Yu,YANG Li-wen等. The effects of PE and PLA microplastics on nitrogen metabolism related functional microorganisms in water[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(1): 278-291.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I1/278

[1] 张丽,潘子亮,郑毅.水环境中新污染物去除技术的研究进展 [J]. 中国资源综合利用, 2024,42(2):115-117. Zhang L, Pan Z L, Zheng Y. Research Progress on Removal Technology of New Pollutants in Water Environment [J]. China Resources Comprehensive Utilization, 2022,42(2):115-117
[2] Hitchcock, James N. Microplastics can alter phytoplankton community composition [J]. Science of The Total Environment, 2022,819,
[3] Ye G, Zhang X, Z, Yan C. et al. Polystyrene microplastics induce microbial dysbiosis and dysfunction in surrounding seawater [J]. Environment International. 2021,156:106724.
[4] Zettler E R, Mincer T J, Amaral-Zettler L A. Life in the “plastisphere”:microbial communities on plastic marine debris [J]. Environmental Science & Technology, 2013,47(13):7137-7146
[5] Rummel C D, Lechtenfeld O J, René K, et al. Conditioning film and early biofilm succession on plastic surfaces [J]. Environmental Science & Technology, 2021,55:11006-11018.
[6] 石凤,周玉婷,李佳霖,等.海洋聚球藻对不同硝酸盐浓度的响应特征 [J]. 海洋科学, 2023,47(11):110-120. Shi F, Zhou Y T, Li J L, et al. Response characteristics of marine Synechococcus under different nitrate concentrations [J]. Marine Sciences, 2023,47(11):110-120.
[7] 张迪涛,张鹏,王司阳,等.基于微生物完整性指数的水生态系统健康评价——以武汉市东西湖区湖泊群为例 [J]. 中国环境科学, 2023,43(6):3055-3067. Zhang D T, Zhang P, Wang S Y, et al. Health assessment of aquatic ecosystem based on microbial integrity index——A case study on lakes in Dongxihu District of Wuhan [J]. China Environmental Science, 2023,43(6):3055-3067.
[8] 杨斌彬,王晓静,陈方舟,等.污染物排放对红树林碳汇影响的研究进展及展望——以福田红树林为例 [J]. 广东化工, 2024,51(7):123-126. Yang B B, Wang X J, Chen F Z, et al. Research Progress and Prospects on the Effects of Pollutant Discharge on the Mangrove Carbon Sink: A Case Study of Futian Mangrove Forest [J]. Guangdong Chemical Industry, 2024,51(7):123-126.
[9] Seeley M E, Song B, Passie R, et al. Microplastics affect sedimentary microbial communities and nitrogen cycling [J]. Nature communications, 2020,11(1):2372.
[10] Zhou, Z D, Hua J F, Xue J H, et al. Polyethylene microplastic and soil nitrogen dynamics: Unraveling the links between functional genes, microbial communities, and transformation processes [J]. Journal of hazardous materials, 2023,458:131857.
[11] Yu Y, Li X, Feng Z, et al. Polyethylene microplastics alter the microbial functional gene abundances and increase nitrous oxide emissions from paddy soils [J]. Journal of Hazardous Materials, 2022,432,128721.
[12] 王嘉昊.微塑料对蚯蚓氧化应激反应及体内微生物群落碳源利用强度的影响 [D]. 哈尔滨:东北农业大学, 2022. Wang J H. Effect of microplastics on the oxidative stress response and the intensity of carbon use by the microbial community in earthworms [D]. Harbin: Dissertation for the Master Degree, 2022.
[13] 文开,柳金涛,王欢,等.微塑料污染对海水微生物群落特征的影响 [J]. 环境科学与技术, 2022,45(5):60-69. Wen K, Liu J T, Wang H, et al. Effects of microplastics pollution on seawater microbial flora [J]. Environmental Science & Technology, 2022,45(5):60-69.
[14] 刘淑丽,胡启武,杨慧林,等.鄱阳湖南矶山湿地微塑料表面微生物分布特征 [J]. 环境科学学报, 2022,42(6):206-214. Liu S L, Hu Q W, Yang H L, et al. Distribution characteristics of microorganisms on microplastic surface in Nanjishan wetland of Poyang Lake [J]. Acta Scientiae Circumstantiae, 2022,42(6):206-214.
[15] 张明,金熠杰,张驰,等.微塑料暴露对铜锈环棱螺抗氧化酶活性及肠道微生物组成的影响 [J]. 杭州师范大学学报(自然科学版), 2024,23(6):565-571. Zhang M, Jin Y J, Zhang C, et al. Impact of microplastic exposure on the antioxidant enzyme activity and intestinal microbiota composition of bellamya aeruginosa [J]. Journal of HangZhou Normal University (Nature Science Edition), 2024,23(6):565-571.
[16] Ye G Z, Zhang X, Yan Changzhou, et al. Polystyrene Microplastics Induce Microbial Dysbiosis and Dysfunction in Surrounding Seawater [J]. Environment international, 2021,156:106724.
[17] Ren X W, Tang J C, Liu X M, et al. Effects of Microplastics on Greenhouse Gas Emissions and the Microbial Community in Fertilized Soil [J]. Environmental Pollution, 2020,256:113347.
[18] Wan Q, Li J, Chen Y. Comparative growth and cellular responses of toxigenic Microcystis exposed to different types of microplastics at various doses [J]. Environment Pollution, 2021,290:117950.
[19] Lu Y F, Huang R H, Wang J L, et al. Effects of polyester microfibers on the growth and toxicity production of bloom-forming cyanobacterium Microcystis aeruginosa [J]. Water, 2022,14:2422.
[20] 弓亚方.覆膜农田土壤的微塑料分布特征及其复合ZnO NPs对绿豆生长的影响 [D]. 西安:西北农林科技大学, 2023. Gong Y F. Distribution characteristics of microplastics inmulched farmland soils and the effect ofcomposite ZnO NPs on mung beans growth [D]. Xian: Northwest A&F University, 2023.
[21] 吕岳骏,李亚芸,王舒淇,等.山西黄河上游枯水期微塑料分布及附着微生物研究 [J]. 中北大学学报(自然科学版), 2023,44(1):65-71. Lv Y J, Li Y Y, Wang S Q, et al. Reasearch on microplastices distribution and attached microorganism in the upstream of the yellow river in shanxi during dry season [J]. Journal of North University of China (Natural Science Edition), 2023,44(1):65-71.
[22] Chen Q, Bao B, Li Y, et al. Effects of marine oil pollution on microbial diversity in coastal waters and stimulating indigenous microorganism bioremediation with nutrients [J]. Regional Studies in Marine Science, 2020,39:101395.
[23] 张雨薇,阿蓉,黎佳茜,等.不同分子量聚乳酸的异养反硝化脱氮性能及脱氮反应机制 [J]. 环境工程学报, 2023,17(5):1599-1611. Zhang Y W, A R, LI J X, et al. Heterotrophic denitrification and denitrification mechanism of polylactic acid with different molecular weight [J]. Chinese Journal of Environmental Engineering, 2023,17(5):1599-1611.
[24] 张浩,孙洁,杨慧颖,等.高密度聚乙烯微塑料胁迫对棉花生长、枯萎病发生和根际细菌群落的影响 [J]. 环境科学, 2023,44(5):2955-2964. Zhang H, Sun J, Yang H Y, et al. Effects of microplastic high-density polyethylene on cotton growth, occurrence of fusarium wilt, and rhizosphere soil bacterial community [J]. Environmental Science, 2023, 44(5):2955-2964.
[25] 康云.水源水库氮素污染物物演变特征及强化好氧脱氮实验研究 [D]. 西安:西安建筑科技大学, 2023. Kang Y. Research on evolution characteristics of nitrogen pollutants in water source reservoirs and experimental of enhanced aerobic denitrification [D]. Xian: Xian University of Architecture and Technology, 2023.
[26] 陈妍汐,查泽宇,毛鑫羽,等.底泥-苦草系统对景观水体氮磷的去除效果及其中微生物群落的结构变化 [J]. 环境工程学报, 2023,17(7): 2391-2401. Chen Y X, Cha Z Y, Mao X Y, et al. Nitrogen and phosphorus removal effect of sediment-Vallisneria natans system on landscape water and changes of microbial community structure [J]. Chinese Journal of Environmental Engineering, 2023,17(7):2391-2401.
[27] 陈慧,马徐,王海波,等.供水PE和PPR塑料管内表面生物膜群落组成及其代谢特征 [J]. 环境科学学报, 2023,43(12):287-295. Chen H, Ma X, Wang H B, et al. Biofilm bacterial community composition and metabolic characteristics on the inner surface of PE and PPR plastic pipes [J]. Acta Scientiae Circumstantiae, 2023,43(12):287-295.
[28] 张岭.疫情背景下不同塑料废弃物塑料际载体效应研究 [D]. 济南:山东师范大学, 2024. Zhang L. Study of the plastisphere on carrier effect of different plastic wastes in the context of COVID-19 [D]. Jinan: Shandong Normal University, 2024.
[29] 相金汛.微塑料对海洋沉积物氮循环和抗生素抗性基因传播的影响 [D]. 辽宁:辽宁师范大学, 2023. Xiang J X. Effects of microplastics on nitrogen cycle and antibiotic resistance genes dissemination in marine sedime [D]. Liaoning: Liaoning Normal University, 2023.
[30] 常焕焕,杨昕怡,于雪,等.基于宏基因组学的亚硝酸盐氧化菌属的新陈代谢功能分析 [J]. 环境工程学报, 2022,16(4):1391-1399. Chang H H, Yang X Y, Yu X, et al, Metabolic function analysis of nitrite oxidizing bacteria based on metagenomics [J]. Chinese Journal of Environmental Engineering, 2022,16(4):1391-1399.

[1]	TANG Kai, SONG Can-hui, CAO Qian-fei, AN Tian-yi, LIU Yang, ZHOU Fan, DU Gui-quan, SUN Fa-qian, CHEN Chong-jun. Pilot application study of the LEP-N-MBR system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(2): 727-735.