一株耐高氨氮异养硝化-好氧反硝化菌的筛选及降解动力学分析

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Abstract A heterotrophic nitrification and aerobic denitrification (HNAD) strain WN1 with significant tolerance against high ammonium concentrations was isolated from a reactor treating high-strength ammonium organic wastewater. Based on colony morphology and 16S rDNA analysis, it was identified as Corynebacterium glutamicum. The growth and metabolic characteristics of WN1 were studied under different initial ammonium concentrations of 500, 1000, 1500, 2000, 2500 and 3000mg/L, respectively. The results showed that strain WN1 could still grow well (48h, OD₆₀₀=3.264) at an initial ammonium concentration of 3000mg/L, corresponding to a free ammonia concentration of 590.2mg/L. As initial ammonium concentrations increased from 500 to 3000mg/L, the maximum ammonium conversion rate (R_m) of strain WN1increased from 15.93 to 43.29mg/(L·h). Additionally, the average conversion rates of ammonium and COD were 7.06~9.36mg/(L·h) and 95.63~199.13mg/(L·h), respectively. Furthermore, the Haldane model was used to characterize the growth and substrate degradation properties of strain WN1under different initial ammonium concentrations (R² = 0.99). Strain WN1had a maximum specific growth rate of u_max = 0.36h^-1, a maximum specific ammonium degradation rate of r_max = 6.45gN/(gDCW·d), and a maximum specific COD degradation rate of r_max = 122.85gCOD/ (gDCW·d). The ammonium inhibition constant K_i of strain WN1was 3749.49mg/L, indicating greater resistance to high ammonium concentrations compared with other autotrophic or heterotrophic ammonium-oxidizing bacteria. The results suggest that strain WN1is promising for treating high-strength ammonium organic wastewater.

Key words： HNAD kinetics high-strength ammonium free ammonia specific degradation rate

Received: 26 June 2024

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X172

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	WANG Nuo
	DAO Yu
	QIN Xing-yu
	SUN De-zhi
	LIU Xin-ying

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WANG Nuo,DAO Yu,QIN Xing-yu等. Isolation and degradation kinetic analysis of a heterotrophic nitrification-aerobic denitrification strain tolerant to high-strength ammonium[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(1): 500-507.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I1/500

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