低碳氮比进水反硝化生物滤池亚硝积累成因

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Abstract In view of the accumulation of nitrite in the effluent of denitrification biofilter in water recycling plant, nitrite accumulation in denitrification filter units of 4recycled water plants was investigated. Two denitrification filter process units of recycled water plant, G (with nitrite accumulation) and W (without nitrite accumulation), were selected as research objects to explore the causes of nitrite accumulation under the condition of low carbon to nitrogen ratio influent. The results showed that operating parameters such as backwashing cycle and mode of filter had little effect on nitrite accumulation. The sludge denitrification rates of plants G and W were 0.51 and 0.92 mgNO₃/(mgVSS·d), respectively. The accumulation of nitrite in the effluent from the filter of Plant G was due to the weak denitrification ability of microorganisms in the filter. When nitrate nitrogen was present in the denitrification process, the denitrification rate of nitrite in the sludge of Plant G was 75% lower than that of plant W, and it was easier to produce nitrite accumulation. When the sludge biomass of Plant G was increased to twice the concentration of plant W, the maximum nitrite denitrification rate still couldn’t reach the level of plant W, so the biomass was not the main reason for the accumulation of nitrite in Plant G. The results of microbial community structure analysis showed that the abundance of Methylotenera in the filter of W plant was 12.9% higher than that of G plant. The denitrification process was mainly completed by denitrifying bacteria using methyl type nutrition, which was the cause of nitrite accumulation happening or not, and it was not necessary to consider the influence of bacteria species using other organic types on nitrite accumulation.

Key words： denitrifying biological filter low carbon nitrogen ratio nitrite accumulation denitrification rate microbial community structure

Received: 08 August 2024

PACS:

X703.1

Corresponding Authors: 李魁晓,教授级高工,kuixiao_li@163.com E-mail: kuixiao_li@163.com

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	WANG Wei
	LIU Kun
	HE Yun
	BAO Fang-bo
	WANG Gang
	XU Qi
	LI Kui-xiao

Cite this article:

WANG Wei,LIU Kun,HE Yun等. Causes of nitrite accumulation in low carbon nitrogen ratio influent denitrification biological filter[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1333-1340.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I3/1333

[1] 杨淇椋,宋凤鸣,古伟,等.反硝化生物滤池+高效气浮工艺用于准Ⅳ类提标改造[J].中国给水排水, 2023,39(12):125-129. Yang Q Q, Song F M, Gu W, et al. Application of denitrifying biofilter and high efficiency air floatation in upgrading of a WWTP for quasi Ⅳ standard[J]. China Water& Wastewater, 2023,39(12):125-129.
[2] 陈雨霞,左丽敏,丁俊杰,等.MBBR池+反硝化生物滤池+臭氧法用于类地表Ⅳ类水排放标准升级改造工程[J].资源节约与环保, 2024,(4):45-48. Chen Y X, Z L M, Ding J J, et al. MBBR tank+denitrification biological filter+ozone method is used in the upgrading project of Class IV water discharge standard of surface[J]. Resources Economization& Environment Protection, 2024,(4):45-48.
[3] 曹相生,付昆明,钱栋,等.甲醇为碳源时C/N对反硝化过程中亚硝酸盐积累的影响[J].化工学报, 2010,(11):6. Cao X S, Fu K M, Qian D, et al. Effect of C/N ratio on nitrite accumulation in dentrifying process with methanol as carbon source[J]. CIESC Journal, 2010,(11):6.
[4] 刘建广,张春阳,张晓健,等.亚硝酸盐氮对臭氧氧化有机物的影响研究[J].中国给水排水, 2007,23(3):85. Liu J G, Zhang C Y, Zhang X J, et al. Impact of nitrite nitrogen on organic matter oxidation by ozone[J]. China Water& Wastewater, 2007,23(3):85.
[5] 田建强.反硝化过程中亚硝酸盐积累的影响因素[J].有色冶金设计与研究, 2008,29(3):42-44. Tian J Q. Influencing factors of nitrite accumulation during denitrification[J]. Nonferrous Metals Engineering& Research, 2008, 29(3):42-44.
[6] 牛萌,王淑莹,杜睿,等.甲醇为碳源短程反硝化亚硝酸盐积累特性[J].中国环境科学, 2017,37(9):3301-3308. Niu M, Wang S Y, Du R, et al. Characteristics of nitrite accumulation during short-path denitrification with methanol as carbon source[J]. China Environmental Science, 2017,37(9):3301-3308.
[7] Wang J, Zhang F, Wang Z, et al. Metagenomic insights into nitrite accumulation in sulfur-based denitrification systems utilizing different electron donors:Functional microbial communities and metabolic mechanisms[J]. Water Research, 2025,270:1-12.
[8] 盛韩微,曹相生.反硝化生物滤池亚硝酸盐积累特性的研究[J].工业安全与环保, 2015,41(12):56-58. Sheng H W, Cao X S. Research on nitrite accumulation characteristics in denitrification biofilter. Industrial Safety and Environmental Protection, 2015,41(12):56-58.
[9] 刘秀红,李健伟,甘一萍,等.反硝化生物滤池在再生水生产中的应用研究[C]//中国环境科学学会学术年会浦华环保优秀论文集, 2013:308. Liu X H, Li J W, Gan Y P, et al. Application study of denitrifying biological filter in reclaimed water production[C]//Proceedings of the Annual Academic Conference of China Environmental Science Society, PuHua Environmental Protection Outstanding Papers Collection, 2013:308.
[10] 国家环境保护总局.水和废水监测分析方法-第4版[M].中国环境科学出版社, 2002. State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. 4th Ed. China Environmental Science Press, 2002.
[11] HJ895-2017水质甲醇和丙酮的测定顶空/气相色谱法[S]. HJ895-2017 Water quality-Determination of methanol and aceton-Headspace gas chromatography[S].
[12] Ge S, Peng Y, Wang S, et al. Nitrite accumulation under constant temperature in anoxic denitrification process:The effects of carbon sources and COD/NO (3)-N.[J]. Bioresource Technology, 2012,114:137-143.
[13] 方先金,邵辉煌.反硝化生物滤池甲醇投加量公式的确定[C]//中国土木工程学会全国排水委员会2013年年会, 2013. Fang X J, Shao H H. Determination of methanol dosage formula for denitrifying biological filter[C]//Proceedings of the 2013 Annual Conference of the National Drainage Committee of China Civil Engineering Society, 2013.
[14] Rissanen A J, Ojala A, Fred T, et al. Methylophilaceae and Hyphomicrobium as target taxonomic groups in monitoring the function of methanol-fed denitrification biofilters in municipal wastewater treatment plants[J]. Journal of Industrial Microbiology& Biotechnology, 2017,44(1):35-47.
[15] Martineau C, Mauffrey F, Villemur R, et al. Comparative analysis of denitrifying activities of hyphomicrobium nitrativorans, hyphomicrobium denitrificans, and hyphomicrobium zavarzinii[J]. Applied& Environmental Microbiology, 2015,81(15):5003-5014.
[16] Mustakhimov I, Kalyuzhnaya M G, Lidstrom M E, et al. Insights into denitrification in methylotenera mobilis from denitrification pathway and methanol metabolism mutants[J]. Journal of Bacteriology, 2013, 195(10):2207-2211.
[17] Du R, Peng Y, Cao S, et al. Mechanisms and microbial structure of partial denitrification with high nitrite accumulation[J]. Applied Microbiology& Biotechnology, 2016,100:2011-2021.
[18] 郭丽芸.反硝化菌功能基因及其分子生态学研究进展[J].微生物学通报, 2011,38(4):583-590. Guo L Y. Advances in functional genes and molecular ecologyin denitrifiers[J]. Microbiology China, 2011,38(4):583-590.
[19] Lu H, Kalyuzhnaya M, Chandran K. Proteomics unravels metabolic strategies employed by nitrate reducing bacteria during growth on different carbon sources[J]. Proceedings of the Water Environment Federation, 2012,2012(12):3683-3698.
[20] 郑晓英,乔露露,王慰,等.碳源对反硝化生物滤池运行及微生物种群的影响[J].环境工程学报, 2018,(5):1434-1442. Zheng X Y, Qiao L L, Wang W, et al. Effects of carbon sources on operation and microbial population of denitrification biological filter[J]. Chinese Journal of Environmental Engineering, 2018,(5):1434-1442.

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