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| Effect of soil iron on anaerobic As(III) oxidation coupling nitrate reduction processes |
| NIU Ru-miao1, YANG Guang1, GAO Yi-fan1, LIANG Lu-yu1, PAN Dan-dan1,2, HUANG Guo-yong1,2, LI Xiao-min1 |
1. Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety, Environmental Research Institute, South China Normal University, Guangzhou 510006, China;
2. Guangdong Provincial Key Laboratory of Integrated Agro-environmental Pollution Control and Management, National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China |
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Abstract In this study, two paddy soils with similar organic matter contents but different iron contents were used to conduct anaerobic microcosm incubation experiments with four treatments, including Control, +NO3-, +As(III), and +As(III)+NO3-. The transformation of arsenic, nitrogen, and iron species, as well as changes in microbial community structure and abundance were investigated in order to elucidate the effect of iron on the microbial As(III) oxidation coupling nitrate reduction processes in soils under anoxic conditions. The results revealed that As(III) oxidation was driven by nitrate reduction, and 35.3% and 43.0% of As(III) were oxidized in the soils with low iron and high iron content, respectively, at the end of incubation. The phosphate-extracted and oxalate-extracted arsenic contents were significantly higher in the soil with high iron content than those in the soil with low iron content. The presence of As(III) slowed down the nitrate reduction process, reduced the accumulation of NO2- and N2O, and promoted the NH4+ production. In addition, the denitrification and dissimilatory nitrate reduction to ammonium (DNRA) processes were faster in the soil with high iron content than those in the soil with low iron content. The presence of nitrate and As(III) decreased the concentrations of dissolved Fe(II) and adsorbed Fe(II) in soils, increased the concentrations of adsorbed total iron, and altered the composition and abundance of soil microbial community. Bacillus, Clostridium, and Planococcaceae were identified as the dominant bacteria during nitrate reduction and As(III) oxidation processes. This study demonstrates that soils with high content of adsorbed iron can facilitate anaerobic As(III) oxidation coupling denitrification/DNRA and enhance the immobilization of As(III) and As(V) by iron (oxyhydr)oxides in soils. These findings provide scientific basis for the regulation of arsenic transformation by iron and nitrogen elements in flooded paddy fields.
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Received: 10 August 2024
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Corresponding Authors:
李晓敏,研究员,xiaomin.li@m.scnu.edu.cn
E-mail: xiaomin.li@m.scnu.edu.cn
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