GAOs丰度变化对聚磷生物膜系统的影响——磷富集性能及代谢特征为例

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Abstract In a sequencing batch reactor with alternating anaerobic/aerobic operation, activated sludge from an oxidation ditch was used as the inoculated sludge for culturing poly-phosphate biofilm enrichment. The potential functions of GAOs in the biofilm phosphorus enrichment system were investigated by examining the effects of the changes in the abundance of glycogen-accumulating organisms (GAOs) in the biofilm on the phosphorus enrichment performance and the metabolic characteristics of the microbial community. The results showed that GAOs became the dominant organisms in the enrichment culture of phosphorus-accumulating biofilm, but they did not adversely affect the phosphorus removal and enrichment of the biofilm system. Due to the significant increase of PHA metabolic activity and poly-P metabolic activity in individual cells of phosphorus-accumulating organisms (PAOs), the biofilm community as a whole was dominated by the phosphorus accumulating metabolism (PAM). GAOs as a dominant bacterial genus in the system, might obtain the reducing power (NADH) required for synthesizing PHA through EMP metabolism, which could provide sufficient energy reserve for the absorption of phosphorus by the PAOs in aerobic conditions, and thus stimulate the phosphorus removal and enrichment effect in the biofilm system. The inorganic phosphorus transport system (pst) and poly-P synthesizing genes (ppk) were up-regulated, so that the biofilm system showed good phosphorus removal and enrichment ability, and the GAOs (Candidatus Competibacter), as a potential denitrifying bacterium, could synchronize with the aerobic denitrification in the biofilm phosphorus-enrichment system.

Key words： phosphorus enrichment from sewage phosphorus-accumulating biofilm glycogen accumulating organisma (GAOs) Candidatus Competibacter metabolic characteristics

Received: 27 August 2024

PACS:

X172

Corresponding Authors: 毕贞,副教授,bizhen@mail.usts.edu.cn E-mail: bizhen@mail.usts.edu.cn

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	BI Zhen
	QIAN Meng-meng
	YUAN Yi-na
	WANG Xue-ling
	SONG Ge
	HUANG Yong

Cite this article:

BI Zhen,QIAN Meng-meng,YUAN Yi-na等. Effects of GAOs abundance to the systemmatic phosphorus enrichment performance and the metabolic characteristics in phosphorus-accumulating biofilm[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1601-1611.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I3/1601

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