微塑料和三氯生复合暴露致斑马鱼脂代谢紊乱

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Abstract In this study, zebrafish were used as a model organism to investigate the effects of combined exposure to polyamide microplastics (PA MPs) and triclosan (TCS) on lipid metabolism in larval zebrafish. The findings demonstrated that PA exhibited a strong adsorption capacity for TCS, with an adsorption rate reaching 74% at 168h. Exposure to PA and TCS from 6hpf to 120hpf resulted in pronounced morphological abnormalities in larval zebrafish, including pericardial edema, swim bladder closure, yolk sac edema, and spinal curvature, with a malformation rate of 13%. Moreover, combined exposure induced the accumulation of reactive oxygen species within the larvae, triggering oxidative stress responses. In the hepatic region, significant lipid accumulation was observed, accompanied by elevated levels of T-CHO and TG. These changes were further correlated with aberrant expression of genes involved in lipid metabolism, confirming that combined exposure to PA and TCS disrupted lipid metabolic processes in zebrafish. Mechanistic investigations revealed that combined exposure led to a reduction in mitochondrial membrane potential and decreased expression of ATP synthase genes, resulting in impaired energy supply and subsequent energy metabolism disruption. Notably, the presence of PA significantly amplified the disruptive effects of TCS on lipid and energy metabolism compared to exposure to either PA or TCS alone.

Key words： microplastics triclosan joint exposure zebrafish lipid metabolism disorder

Received: 28 August 2024

PACS:

X503.2

Corresponding Authors: 钱秋慧,讲师,qhqian@usts.edu.cn E-mail: qhqian@usts.edu.cn

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	CHENG Guan-chen
	LIU Xing-cheng
	WANG Hui-li
	QIAN Qiu-hui

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CHENG Guan-chen,LIU Xing-cheng,WANG Hui-li等. The combined exposure to microplastics and triclosan disrupts lipid metabolism in zebrafish[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1636-1646.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I3/1636

[1] Andrady A L. Microplastics in the marine environment[J]. Marine Pollution Bulletin, 2011,62(8):1596-1605.
[2] Prabhu P P, Pan K, Krishnan J N. Microplastics:Global occurrence, impact, characteristics and sorting[J]. Frontiers in Marine Science, 2022,9:893641.
[3] Lasee S, Mauricio J, Thompson W A, et al. Microplastics in a freshwater environment receiving treated wastewater effluent[J]. Integrated Environmental Assessment and Management, 2017,13(3):528-532.
[4] Ding J, Li J, Sun C, et al. Detection of microplastics in local marine organisms using a multi-technology system[J]. Analytical Methods, 2019,11(1):78-87.
[5] Vivekanand A C, Mohapatra S, Tyagi V K. Microplastics in aquatic environment:Challenges and perspectives[J]. Chemosphere, 2021,282:131151.
[6] Shim W J, Thomposon R C. Microplastics in the ocean[J]. Archives of Environmental Contamination and Toxicology, 2015,69:265-268.
[7] 盛诚,王馨颖,易珍,等.不同材质微塑料对三氯生的吸附及其在斑马鱼体内累积分布的影响[J].生态毒理学报, 2020,15(5):108-117. Sheng C, Wang X Y, Yi Z, et al. Adsorption of triclosan by microplastics of different materials and their effects on accumulation and distribution in zebrafish[J]. Journals of Ecotoxicology, 2020,15(5):108-117.
[8] Fu L, Li J, Wang G, et al. Adsorption behavior of organic pollutants on microplastics[J]. Ecotoxicology and Environmental Safety, 2021,217:112207.
[9] Martín J, Santos J L, Aparicio I, et al. Microplastics and associated emerging contaminants in the environment:Analysis, sorption mechanisms and effects of co-exposure[J]. Trends in Environmental Analytical Chemistry, 2022,35:e00170.
[10] Llorca M, Schirinzi G, Martínez M, et al. Adsorption of perfluoroalkyl substances on microplastics under environmental conditions[J]. Environmental Pollution, 2018,235:680-691.
[11] 韩晓雯,徐婕妤,王伟伟,等.三氯生和双酚A对斑马鱼神经毒性的比较研究[J].中国环境科学, 2024,44(2):1111-1121. Han X W, Xu J Y, Wang W W, et al. Comparative study on neurotoxicity of triclosan and bisphenol A in zebrafish[J]. China Environmental Science, 2024,44(2):1111-1121.
[12] Dann A B, Hontela A. Triclosan:Environmental exposure, toxicity and mechanisms of action[J]. Journal of Applied Toxicology, 2011,31(4):285-311.
[13] Milanović M,Đurić L, Milošević N, et al. Comprehensive insight into triclosan-from widespread occurrence to health outcomes[J]. Environmental Science and Pollution Research, 2023,30(10):25119-25140.
[14] Fair P A, Lee H B, Adams J, et al. Occurrence of triclosan in plasma of wild Atlantic bottlenose dolphins (Tursiops truncatus) and in their environment[J]. Environmental Pollution, 2009,157(8/9):2248-2254.
[15] Macherius A, Lapen D R, Reemtsma T, et al. Triclocarban, triclosan and its transformation product methyl triclosan in native earthworm species four years after a commercial-scale biosolids application[J]. Science of the Total Environment, 2014,472:235-238.
[16] 王杨,吴国辉,钱秋慧,等.三氯生对斑马鱼发育和脂质代谢的影响[J].中国环境科学, 2022,42(3):1394-1400. Wang Y, Wu G H, Qian Q H, et al. The effect of triclosan on the development and lipid metabolism of zebrafish[J]. China Environmental Science, 2022,42(3):1394-1400.
[17] Huang W, Xie P, Cai Z. Lipid metabolism disorders contribute to hepatotoxicity of triclosan in mice[J]. Journal of Hazardous Materials, 2020,384:121310.
[18] Thapa B R, Walia A. Liver function tests and their interpretation[J]. The Indian Journal of Pediatrics, 2007,74:663-671.
[19] Yan J, Li J Y, Wang Y, et al. Deciphering the molecular mediators of triclosan-induced lipid accumulation:Intervention via short-chain fatty acids and miR-101a[J]. Environmental Pollution, 2024,343:123153.
[20] Jia Y W, Huang Z, Hu L X, et al. Occurrence and mass loads of biocides in plastic debris from the Pearl River system, South China[J]. Chemosphere, 2020,246:125771.
[21] Cortés-Arriagada D, Ortega D E. Interaction mechanism of triclosan on pristine microplastics[J]. Science of the Total Environment, 2023, 891:164470.
[22] Yueh M F, Tukey R H. Triclosan:A widespread environmental toxicant with many biological effects[J]. Annual Review of Pharmacology and Toxicology, 2016,56(1):251-272.
[23] Jagini S, Konda S, Bhagawan D, et al. Emerging contaminant (triclosan) identification and its treatment:a review[J]. SN Applied Sciences, 2019,1:1-15.
[24] Ma J, Zhao J, Zhu Z, et al. Effect of microplastic size on the adsorption behavior and mechanism of triclosan on polyvinyl chloride[J]. Environmental Pollution, 2019,254:113104.
[25] Jomova K, Raptova R, Alomar S Y, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants:Chronic diseases and aging[J]. Archives of Toxicology, 2023,97(10):2499-2574.
[26] Ighodaro O M, Akinloye O A. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX):Their fundamental role in the entire antioxidant defence grid[J]. Alexandria Journal of Medicine, 2018,54(4):287-293.
[27] Smith A M, Zeve D R, Grisel J J, et al. Neonatal alcohol exposure increases malondialdehyde (MDA) and glutathione (GSH) levels in the developing cerebellum[J]. Developmental Brain Research, 2005, 160(2):231-238.
[28] Fowler S D, Greenspan P. Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections:Comparison with oil red O[J]. Journal of Histochemistry& Cytochemistry, 1985,33(8):833-836.
[29] Feng R, Luo C, Li C, et al. Free fatty acids profile among lean, overweight and obese non-alcoholic fatty liver disease patients:A case-control study[J]. Lipids in Health and Disease, 2017,16:1-9.
[30] Lichtenstein L, Kersten S. Modulation of plasma TG lipolysis by Angiopoietin-like proteins and GPIHBP1[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2010, 1801(4):415-420.
[31] Chirala S S, Wakil S J. Structure and function of animal fatty acid synthase[J]. Lipids, 2004,39(11):1045-1053.
[32] Lupu R, Menendez J A. Pharmacological inhibitors of fatty acid synthase (FASN)-catalyzed endogenous fatty acid biogenesis:A new family of anti-cancer agents?[J]. Current Pharmaceutical Biotechnology, 2006,7(6):483-494.
[33] Van der Bliek A M, Sedensky M M, Morgan P G. Cell biology of the mitochondrion[J]. Genetics, 2017,207(3):843-871.
[34] Zorova L D, Popkov V A, Plotnikov E Y, et al. Mitochondrial membrane potential[J]. Analytical Biochemistry, 2018,552:50-59.
[35] Zorova L D, Popkov V A, Plotnikov E J, et al. Functional significance of the mitochondrial membrane potential[J]. Biochemistry (Moscow), Supplement Series A:Membrane and Cell Biology, 2018,12:20-26.
[36] Bonora M, Patergnani S, Rimessi A, et al. ATP synthesis and storage[J]. Purinergic Signalling, 2012,8:343-357.
[37] Junge W, Nelson N. ATP synthase[J]. Annual Review of Biochemistry, 2015,84(1):631-657.
[38] Morth J P, Pedersen B P, Toustrup-Jensen M S, et al. Crystal structure of the sodium-potassium pump[J]. Nature, 2007,450(7172):1043-1049.
[39] Nůsková H, Mráček T, Mikulová T, et al. Mitochondrial ATP synthasome:Expression and structural interaction of its components[J]. Biochemical and Biophysical Research Communications, 2015, 464(3):787-793.
[40] Jonckheere A I, Smeitink J A M, Rodenburg R J T. Mitochondrial ATP synthase:Architecture, function and pathology[J]. Journal of Inherited Metabolic Disease, 2012,35:211-225.
[41] Oliveira R, Domingues I, Koppe Grisolia C, et al. Effects of triclosan on zebrafish early-life stages and adults[J]. Environmental Science and Pollution Research, 2009,16:679-688.
[42] Wang L, Mao B, He H, et al. Comparison of hepatotoxicity and mechanisms induced by triclosan (TCS) and methyl-triclosan (MTCS) in human liver hepatocellular HepG2 cells[J]. Toxicology Research, 2019,8(1):38-45.
[43] Wu X, Liu P, Huang H, et al. Adsorption of triclosan onto different aged polypropylene microplastics:Critical effect of cations[J]. Science of the Total Environment, 2020,717:137033.
[44] Zabłocka-Słowińska K, Płaczkowska S, Skórska K, et al. Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism[J]. PLoS One, 2019,14(4):e0215246.
[45] Zhao T, Wu K, Hogstrand C, et al. Lipophagy mediated carbohydrate-induced changes of lipid metabolism via oxidative stress, endoplasmic reticulum (ER) stress and ChREBP/PPARγ pathways[J]. Cellular and Molecular Life Sciences, 2020,77:1987-2003.
[46] Varela L, Vázquez M J, Cordido F, et al. Ghrelin and lipid metabolism:Key partners in energy balance[J]. Journal of Molecular Endocrinology, 2011,46(2):R43-R63.
[47] Ellertsdottir E, Ganz J, Dürr K, et al. A mutation in the zebrafish Na, K-ATPase subunit atp1a1a. 1provides genetic evidence that the sodium potassium pump contributes to left-right asymmetry downstream or in parallel to nodal flow[J]. Developmental Dynamics, 2006,235(7):1794-1808.
[48] Song X, Wu X, Song X, et al. Sorption and desorption of petroleum hydrocarbons on biodegradable and nondegradable microplastics[J]. Chemosphere, 2021,273:128553.
[49] Li Y, Li M, Li Z, et al. Effects of particle size and solution chemistry on triclosan sorption on polystyrene microplastic[J]. Chemosphere, 2019,231:308-314.