异养硝化细菌<i>Pseudomonas stutzeri</i> strain NP3的同步脱氮除磷特性及代谢机制

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2303 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract In order to address the issues of complex processes and high infrastructure and operational costs in simultaneous nitrogen and phosphorus removal, a heterotrophic nitrifying strain NP3 exhibiting simultaneous nitrogen and phosphorus removal capabilities was isolated from activated sludge in this study. Strain NP3 was identified as Pseudomonas stutzeri by 16S rRNA sequence analysis, and its nitrogen and phosphorus removal characteristics and mechanisms were investigated. It was showed that strain NP3 was able to utilize ammonium, nitrate, and nitrite as the sole nitrogen source for efficient nitrogen and phosphorus removal under aerobic conditions. The accumulation of intermediate products during the reaction process was minimal, and nitrogen and phosphorus were primarily removed through assimilation. The growth and metabolic rates followed the order: NH₄⁺-N >NO₂⁻-N >NO₃⁻-N. Under the optimal growth conditions of sodium citrate as the carbon source, C/N=10, T=30℃, pH =7, and r=160r/min, the maximum removal rates of ammonia nitrogen and phosphate were almost 100%. Furthermore, successful amplification of denitrification and polyphosphate genes (nosZ, nirS, ppk) further confirmed the simultaneous nitrogen and phosphorus removal capability of strain NP3. X-ray Photoelectron Spectroscopy (XPS) analysis demonstrated that the functional groups on the extracellular polymeric substances (EPS) surface could adsorb different forms of phosphorus such as C-PO₃/P-C, PO₄^3-/HPO₄^2-, acting as phosphorus transfer stations.³¹P nuclear magnetic resonance (NMR) results further indicated that there was a large effect of EPS on phosphorus fugitive morphology, with pyrophosphate being the main phosphorus species in the presence of EPS, whereas orthophosphate and orthophosphate diester were the major phosphorus forms after EPS extraction.

Key words： heterotrophic nitrification aerobic denitrification simultaneous nitrogen and phosphorus removal extracellular polymeric substances

Received: 11 September 2024

PACS:

X172

Corresponding Authors: 杨垒,责任作者,副教授,yangleigps@xauat.edu.cn E-mail: yangleigps@xauat.edu.cn

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	MENG Hong-yan
	YANG Lei
	LI Yu-cai
	ZHANG Sheng-jing
	LU Hao-qi
	LIANG Pan
	REN Yong-xiang

Cite this article:

MENG Hong-yan,YANG Lei,LI Yu-cai等. Simultaneous nitrogen and phosphorus removal characteristics and metabolic mechanism of heterotrophic nitriying bacterium Pseudomonas stutzeri Strain NP3[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 1901-1910.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/1901

[1] Xu N, Liao M, Liang Y, et al. Biological nitrogen removal capability and pathways analysis of a novel low C/N ratio heterotrophic nitrifying and aerobic denitrifying bacterium (Bacillus thuringiensis strain WXN-23)[J]. Environmental Research, 2021,195:110797.
[2] 刘妍,袁林江,陈希,等.一株异养硝化-好氧反硝化-积累磷的细菌Klebsiella pneumoniae A15的筛出及其特性研究[J].中国环境科学, 2024,44(4):2286-2296. Liu Y, Yuan L J, Chen X, et al. Sieving and characterization of a heterotrophic nitrifying-aerobic denitrifying-phosphorus-accumulating bacterial strain, Klebsiella pneumoniae A15[J]. China Environmental Science, 2024,44(4):2286-2296.
[3] 李艳丽,杨垒,张志昊,等.好氧反硝化细菌Burkholderia sp. ZH8的脱氮特性与生物强化作用[J].中国环境科学, 2024,44(8):4282-4291. Li Y L, Yang L, Zhang Z H, et al. Nitrogen removal characteristics and bioenhancement effects of aerobic denitrifying bacteria Burkholderia sp. ZH8[J]. China Environmental Science, 2024,44(8):4282-429.
[4] Li B, Jing F, Wu D, et al. Simultaneous removal of nitrogen and phosphorus by a novel aerobic denitrifying phosphorus-accumulating bacterium, Pseudomonas stutzeri ADP-19[J]. Bioresource Technology, 2021,321:124445.
[5] Huang M, CuiY, Huang J, et al. A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal[J]. Water Research, 2022,221:118823.
[6] Zhang M, Pan L, Su C, et al. Simultaneous aerobic removal of phosphorus and nitrogen by a novel salt-tolerant phosphate-accumulating organism and the application potential in treatment of domestic sewage and aquaculture sewage[J]. Science of the Total Environment, 2021,758:143580.
[7] Sun S, Han J, Hu M, et al. Removal of phosphorus from wastewater by Diutina rugosa BL3:Efficiency and pathway[J]. Science of the Total Environment, 2021,801:149751.
[8] Tan X, Gao W, Duan Z, et al. Synthesis of novel algal extracellular polymeric substances (EPS)-based hydrogels for the efficient removal and recovery of phosphorus from contaminated waters:Development, characterisation, and performance[J]. Journal of Environmental Chemical Engineering, 2023,11:109044.
[9] Zhang J, Wu P, Hao B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011,102(21):9866-9869.
[10] Duan J, Fang H, Su B, et al. Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater[J]. Bioresource Technology, 2015,179:421-428.
[11] Jiang L, Wang M, Wang Y, et al. The condition optimization and mechanism of aerobic phosphorus removal by marine bacterium Shewanella sp[J]. Chemical Engineering Journal, 2018,345:611-620.
[12] Yang L, Wang X, Cui S, et al. Simultaneous removal of nitrogen and phosphorous by heterotrophic nitrification-aerobic denitrification of a metal resistant bacterium Pseudomonas putida strain NP5[J]. Bioresource Technology, 2019,285:121360.
[13] Yang L, Lu H, Wang Y, et al. Nitrogen removal characteristics and Cr (VI) tolerance mechanisms of heterotrophic nitrifying bacterium Pseudomonas putida strain LX1[J]. Journal of Water Process Engineering, 2024,64:105647.
[14] He T, Li Z, Sun Q, et al. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion[J]. Bioresource Technology, 2016,200:493-499.
[15] Chen S, He S, Wu C, et al. Characteristics of heterotrophic nitrification and aerobic denitrification bacterium Acinetobacter sp. T1 and its application for pig farm wastewater treatment[J]. Journal of Bioscience and Bioengineering, 2019,127(2):201-205.
[16] Cui Y, Cui Y W, Huang J L. A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification[J]. Bioresource Technology, 2021,333:125189.
[17] Wang X, Wang W, Zhang Y, et al. Simultaneous nitrification and denitrification by a novel isolated Pseudomonas sp. JQ-H3 using polycaprolactone as carbon source[J]. Bioresource Technology, 2019, 288:121506.
[18] Li Y, Wang Y, Fu L, et al. Aerobic-heterotrophic nitrogen removal through nitrate reduction and ammonium assimilation by marine bacterium Vibrio sp. Y1-5[J]. Bioresource Technology, 2017,230:103-111.
[19] Wan C, Yang X, Lee D J, et al. Aerobic denitrification by novel isolated strain using NO₂^--N as nitrogen source[J]. Bioresource Technology 2011,102(15):7244-7248.
[20] Zhang Q L, Liu Y, Ai G M, et al. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7[J]. Bioresource Technology, 2012,108:35-44.
[21] Zhou Y, Oehmen A, Lim M, et al. The role of nitrite and free nitrous acid (FNA) in wastewater treatment plants[J]. Water Research, 2011, 45(15):4672-4682.
[22] Chen J, Gu S, Hao H, et al. Characteristics and metabolic pathway of Alcaligenes sp. TB for simultaneous heterotrophic nitrification-aerobic denitrification[J]. Applied Microbiology and Biotechnology, 2016,100(22):9787-9794.
[23] Wei B, Luo X, Ma W, et al. Biological nitrogen removal and metabolic characteristics of a novel cold-resistant heterotrophic nitrification and aerobic denitrification Rhizobium sp. WS7[J]. Bioresource Technology, 2022,362:127756.
[24] Xia L, Li X, Fan W, et al. Heterotrophic nitrification and aerobic denitrification by a novel Acinetobacter sp. ND7 isolated from municipal activated sludge[J]. Bioresource Technology, 2020,301:122749.
[25] Wu L, Ding X, Lin Y, et al. Nitrogen removal by a novel heterotrophic nitrification and aerobic denitrification bacterium Acinetobacter calcoaceticus TY1 under low temperatures[J]. Bioresource Technology, 2022,353:127148.
[26] Fan Y, Su J, Zheng Z, et al. Denitrification performance and mechanism of a novel isolated Acinetobacter sp. FYF8 in oligotrophic ecosystem[J]. Bioresource Technology, 2021,320:124280.
[27] Carneiro Fidélis Silva L, Santiago Lima H, Antônio de Oliveira Mendes T, et al. Heterotrophic nitrifying/aerobic denitrifying bacteria:Ammonium removal under different physical-chemical conditions and molecular characterization[J]. Journal of Environmental Management, 2019,248:109294.
[28] Ma F, Sun Y, Li A. et al. Activation of accumulated nitrite reduction by immobilized Pseudomonas stutzeri T13 during aerobic denitrification[J]. Bioresource Technology, 2015,187:30-36.
[29] Rout P R, Bhunia P, Dash R R. Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal[J]. Bioresource Technology 2017,244:484-495.
[30] Wu C, Chen Y, Qian Z, et al. The effect of extracellular polymeric substances (EPS) of iron-oxidizing bacteria (Ochrobactrum EEELCW01) on mineral transformation and arsenic (As) fate[J]. Journal of Environmental Sciences, 2023,130:187-196.
[31] Hou R, Yang P, Qian S, et al. Understanding the mechanism of denitrifying phosphorus removal from the perspective of intracellular carbon source and extracellular polymeric substances characteristics[J]. Journal of Cleaner Production, 2022,367:133115.
[32] Yuan S J, Sun M, Sheng G P, et al. Identification of key constituents and structure of the extracellular polymeric substances excreted by Bacillus megaterium TF10 for their flocculation capacity[J]. Environmental Science& Technology, 2011,45(3):1152-1157.
[33] Zheng C, Wu Q, Hu X, et al. Adsorption behavior of heavy metal ions on a polymer-immobilized amphoteric biosorbent:Surface interaction assessment[J]. Journal of Hazardous Materials, 2021,403:123801.
[34] Yan H, Huang M, Wang J, et al. Difference in calcium ion precipitation between free and immobilized Halovibrio mesolongii HMY2[J]. Journal of Environmental Sciences, 2022,122:184-200.
[35] Li L, Pang H, He J, et al. Characterization of phosphorus species distribution in waste activated sludge after anaerobic digestion and chemical precipitation with Fe³⁺ and Mg²⁺[J]. Chemical Engineering Journal, 2019,373:1279-1285.
[36] Huang W, Cai W, Huang H, et al. Identification of inorganic and organic species of phosphorus and its bio-availability in nitrifying aerobic granular sludge[J]. Water Research, 2015,68:423-431.
[37] Jørgensen C, Jensen H S, Andersen FØ, et al. Occurrence of orthophosphate monoesters in lake sediments:significance of myo-and scyllo-inositol hexakisphosphate[J]. Journal of Environmental Monitoring, 2011,13(8):2328-2334.
[38] Wang Q, He J. Complete nitrogen removal via simultaneous nitrification and denitrification by a novel phosphate accumulating Thauera sp. strain SND5[J]. Water Research, 2020,185:116300.
[39] Long X, Tang R, Fang Z, et al. The roles of loosely-bound and tightly-bound extracellular polymer substances in enhanced biological phosphorus removal[J]. Chemosphere, 2017,189:679-688.
[40] Han J, Qiu Q, Gao M, et al. Phosphorus removal from municipal wastewater through a novel Trichosporon asahii BZ:Performance and mechanism[J]. Chemosphere, 2022,298:134329.