水温变化对金沙江特有鱼类岩原鲤血清生化指标及肠道微生物的影响

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Abstract A controlled experiment was conducted to investigate temperature-induced alterations in serum biochemical indices and gut microbiota of endemic fish (Procypris rabaudi) in Jinsha River, a cascade hydropower development river in southwest China. Three temperature treatments (16℃, 20℃, 24℃) were established with exposure durations of 24h and 10d. Results showed that compared to the ambient temperature group, the serum antioxidant enzyme activity was promoted in the low temperature group after 24h but inhibited after 10d. The decrease in lysozyme (LZM) activity in the low temperature group of 10d (67.12%) was more significant than that of 24h group (52.04%) relative to the ambient temperature group. And the concentrations of glucose (GLU) and CORTISOL were increased significantly in the low temperature groups both 24h and 10d compared with those in the ambient temperature groups. The Chao1index of the low temperature group at 24h were 23.22% and 26.36% lower than those of the ambient temperature and high temperature groups, respectively. The alpha diversity was found to stabilize in the low temperature group after 10d. Compared to 24h, the difference in gut microbiota community structure under different temperature conditions after 10d was smaller, and the low temperatures significantly affected the community composition of the gut microbiota. Co-occurrence network analysis revealed that microbial interactions were simplified and weakened in the low temperature group (24h), whereas prolonged thermal adaptation (10d) was associated with network stabilization. Significant correlations were established between Proteobacteria, Bacteroidetes, and Firmicutes with antioxidant enzymes and LZM activity.

Key words： the cascade hydropower development water temperature Procypris rabaudi serum biochemical indices gut microbiota

Received: 26 September 2024

PACS:

X522

Corresponding Authors: 陈娟,责任作者,教授,chenjuanmn@hhu.edu.cn E-mail: chenjuanmn@hhu.edu.cn

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	CHEN Dan-li
	YANG Xiao-ling
	LIU Shuai-shuai
	ZHANG Bo
	CUI Ge
	GAO Han
	CHEN Juan

Cite this article:

CHEN Dan-li,YANG Xiao-ling,LIU Shuai-shuai等. Effects of water temperature changes on serum biochemical indices and gut microbiota of Endemic Fish (Procypris rabaudi) in Jinsha River[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 2288-2297.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/2288

[1] He Y F, Wang J W, Lek-Ang S, et al. Predicting assemblages and species richness of endemic fish in the upper Yangtze River[J]. Science of the Total Environment, 2010,408(19):4211-4220.
[2] 刘军.长江上游特有鱼类受威胁及优先保护顺序的定量分析[J].中国环境科学, 2004,24(4):395-399. Liu J. A quantitative analysis on threat and priority of conservation order of the endemic fishes in upper reaches of the Yangtze River[J]. China Environmental Science, 2004,24(4):395-399.
[3] 李倩,李翀,骆辉煌.长江上游珍稀特有鱼类生态水温目标研究[J].中国水利水电科学研究院学报, 2012,10(2):86-91. Li Q, Li C, Luo H H. Ecological target water temperature of rare and endemic fish in Yangtze River upstream[J]. Journal of China Institute of Water Resources and Hydropower Research, 2012,10(2):86-91.
[4] 陶雨薇,王远坤,王栋,等.三峡水库坝下水温变化及其对鱼类产卵影响[J].水力发电学报, 2018,37(10):48-55. Tao Y W, Wang Y K, Wang D, et al. Assessing water temperature variations and impacts on fish spawning downstream of Three Gorges dam[J]. Journal of Hydroelectric Engineering, 2018,37(10):48-55.
[5] Young P S, Cech J J, Thompson L C. Hydropower-related pulsed-flow impacts on stream fishes:a brief review, conceptual model, knowledge gaps, and research needs[J]. Reviews in Fish Biology and Fisheries, 2011,21:713-731.
[6] 王渝栋,崔磊,李茜,等.金沙江中游干流鱼类资源现状及梯级开发对其产生的影响分析[J].水生生物学报, 2024,48(8):1425-1435. Wang Y D, Cui L, Li Q, et al. The current status of fishery resources in the middle reaches of Jinsha River and the impact of cascade development[J]. Acta Hydrobiologica Sinica, 2024,48(8):1425-1435.
[7] Islam M J, Slater M J, Thiele R, et al. Influence of extreme ambient cold stress on growth, hematological, antioxidants, and immune responses in European seabass, Dicentrarchus labrax acclimatized at different salinities[J]. Ecological Indicators, 2021,122:107280.
[8] 丰超杰,张颖,张永泉,等.急性高温胁迫对黑龙江茴鱼血清生化指标、消化酶、抗氧化酶活性及其基因表达的影响[J].淡水渔业, 2023,53(6):37-45. Feng C J, Zhang Y, Zhang Y Q et al. Effects of acute temperature stress on serum biochemical indexes, digestiveenzymes, antioxidant enzymes and gene expression of Thymallus arcticus grubei[J]. Freshwater Fisheries, 2023,53(6):37-45.
[9] Saurabh S, Sahoo P K. Lysozyme:an important defence molecule of fish innate immune system[J]. Aquaculture Research, 2008,39(3):223-239.
[10] Ghanbari M, Kneifel W, Domig K J. A new view of the fish gut microbiome:advances from next-generation sequencing[J]. Aquaculture, 2015,448:464-475.
[11] Song Z M, Liu J Y, Zhuang P, et al. Influence of low-temperature stress on the antioxidant enzymes activities and malondialdehyde contents in liver of juvenile Siganus guttatas[J]. Marine Fisheries, 2015,37(2):142-150.
[12] Raida M K, Buchmann K. Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination[J]. Diseases of Aquatic Organisms, 2007,77(1):41-52.
[13] Li R X, Amenyogbe E, Lu Y, et al. Effects of low-temperature stress on serum biochemical indicators, intestinal microbiome, and transcription of juvenile golden pompano (Trachinotus ovatus)[J]. Aquaculture International, 2024,20:1-28.
[14] Zhu T B, Li X M, Wu X B, et al. Temperature acclimation alters the thermal tolerance and intestinal heat stress response in a Tibetan fish Oxygymnocypris Stewarti [J]. Frontiers in Microbiology, 2022,13:898145.
[15] Zhou C W, Duan Y T, Li J T, et al. Decoding the intestinal response to heat stress in Gymnocypris eckloni:Insights from a thorough analysis of microbiome and transcriptome[J]. Aquaculture, 2024,591:741112.
[16] Li L K, Wang Y, Huang Y Q, et al. Multi-omics approach to study the dual effects of novel proteins on the intestinal health of juvenile largemouth bass (Micropterus salmoides) under an alternate feeding strategy[J]. Frontiers in Immunology, 2023,14:1110696.
[17] Huang P, Cao L P, Du J L, et al. Effects of prometryn exposure on hepatopancreas oxidative stress and intestinal flora in Eriocheir sinensis(Crustacea:Decapoda)[J]. Antioxidants, 2023,12(8):548.
[18] 张陵,郭文献,李泉龙.长江中下游水电开发对河流水文情势的影响研究[J].中国农村水利水电, 2019,(9):94-99. Zhang L, Guo W X, Li Q L. Research on hydro-ecological effect of damming rivers in mid-lower Yangtze[J]. China Rural Water and Hydropower, 2019,(9):94-99.
[19] 赵旭,王浩,赵志龙,等.金沙江下游梯级水电工程库区生态系统服务价值演化及权衡协同关系[J].生态与农村环境学报, 2024, 40(1):44-54. Zhao X, Wang H, Zhao Z L, et al. Evolution and tradeoff/synergy relationship of ecosystem services value in cascade hydropower project reservoir area of the lower reaches of Jinsha River[J]. Journal of Ecology and Rural Environment, 2024,40(1):44-54.
[20] 熊飞,张伟,翟东东,等.蓄水后向家坝库区鱼类物种、分类和功能多样性变化[J].湖泊科学, 2024,36(1):200-212. Xiong F, Zhang W, Zhai D D, et al. Changes of species, taxonomic and functional diversity of fish assemblages in the Xiangjiaba reservoir of the lower Jinsha River after impoundment[J]. Journal of Lake Sciences, 2024,36(1):200-212.
[21] Song J, Chang L J, Wang D. Multi-locus genomic analysis reveals the genetic diversity and population structure of the rock carp (Procypris rabaudi) in the upper Yangtze River[J]. Biochemical Systematic and Ecology, 2016,66:86-93.
[22] 朱其广,杨志,唐会元,等.金沙江中下游特有鱼类的生物学、生态学特征及其保护对策[J].长江流域资源与环境, 2021,30(7):1593-1602. Zhu Q G, Yang Z, Tang H Y, et al. Biological and ecological characteristics of endemic fishes in the middle and lower reaches of Jinsha River and their conservation measures[J]. Resources and Environment in the Yangtze Basin, 2021,30(7):1593-1602.
[23] 周扬,王宁,温潇雄,等.长江上游梯级水电开发的水温累积影响及生态效应[J].水力发电学报, 2024,43(11):1-16. Zhou Y, Wang N, Wen X X, et al. Cumulative influences and ecological effects of cascade hydropower development on water temperature in upper Yangtze River[J]. Journal of Hydroelectric Engineering, 2024,43(11):1-16.
[24] 陈金凤,曾凌,李雨,等.金沙江下游水温变化规律探讨[J].长江科学院院报, 2024,41(6):200-206. Chen J F, Zeng L, Li Y, et al. Water temperature variations in lower Jingsha River[J]. Journal of Changjiang River Scientific Research, 2024,41(6):200-206.
[25] Chen J, Zhang J J, Wang C, et al. Nitrate input inhibited the biodegradation of erythromycin through affecting bacterial network modules and keystone species in lake sediments[J]. Journal of Environmental Management, 2024,355:120530.
[26] Liu L M, Chen H H, Liu M, et al. Response of the eukaryotic plankton community to the cyanobacterial biomass cycle over 6years in two subtropical reservoirs[J]. The ISME journal, 2019,13(9):2196-2208.
[27] Lv H R, Liu Y L, Li H D, et al. Modulation of antioxidant enzymes, heat shock protein, and intestinal microbiota of large yellow croaker (Larimichthys crocea) under acute cold stress[J]. Frontiers in Marine Science, 2021,8:725899.
[28] An K W, Kim N N, Shin H S, et al. Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli)[J]. Comparative Biochemistry and Physiology Part A:Molecular& Interactive Physiology, 2010,156(2):262-268.
[29] Yang S Y, Zhang C Y, Xu W Q, et al. Heat stress decreases intestinal physiological function and facilitates the proliferation of harmful intestinal microbiota in sturgeons[J]. Frontiers in Microbiology, 2022,13:755369.
[30] de Mello M M, Piedade A E, Pereira de Faria C D, et al. Acute low temperature and lipopolysaccharide differentially modulated the innate immune and antioxidant responses in a subtropical fish, the pacu (Piaractus mesopotamicus)[J]. Fish Physiology and Biochemistry, 2025,51(1):1-1.
[31] Reid C H, Patrick P H, Rytwinski T, et al. An updated review of cold shock and cold stress in fish[J]. Journal of Fish Biology, 2022,100(5):1102-1137.
[32] Mommsen T P, Vijayan M M, Moon T W. Cortisol in teleosts:dynamics, mechanisms of action, and metabolic regulation[J]. Reviews in Fish Biology and Fisheries, 1999,9:211-268.
[33] 施兆鸿,谢明媚,彭士明,等.温度胁迫对银鲳(Pampus argenteus)幼鱼消化酶活性及血清生化指标的影响[J].渔业科学进展, 2016,37(5):30-37. Shi Z H, Xie M M, Peng S M, et al. Effects of temperature stress on activities of digestive enzymes and serum effects of temperature stress on activities of digestive enzymes and serum[J]. Progress in Fishery Sciences, 2016,37(5):30-37.
[34] Steiner K, Laroche O, Walker S P, et al. Effects of water temperate on the gut microbiome and physiology of Chinook salmon (Oncorhynchus tshawytscha) reared in a freshwater recirculating system[J]. Aquaculture, 2022,560:738529.
[35] Ghosh S K, Wong M K, Hyodo S, et al. Temperature modulation alters the gut and skin microbial profiles of chum salmon (Oncorhynchus keta)[J]. Frontiers in Marine Science, 2022,9:1027621.
[36] Navarro-Guillen C, Degre Lorentsen E, Perera E, et al. Feeding and water temperature modulate the potential activity of gut microbiota in greater amberjack juveniles (Seriola dumerili)[J]. Frontiers in Marine Science, 2023,10:1236305.
[37] Wang X Z, Tang B, Luo X, et al. Effects of temperature, diet and genotype-induced variations on the gut microbiota of abalone[J]. Aquaculture, 2020,524:735269.
[38] Huyben D, Sun L, Moccia R, et al. Dietary live yeast and increased water temperature influence the gut microbiota of rainbow trout[J]. Journal of Applied Microbiology, 2018,124(6):1377-1392.
[39] Idenyi J N, Abanikannda M F, Huber D H, et al. Genome‐wide insights into whole gut microbiota of rainbow trout, Oncorhynchus mykiss, fed plant proteins and camelina oil at different temperature regimens[J]. Journal of the World Aquaculture Society, 2024,55(2):e13028.
[40] Kashinskaya E N, Simonov E P, Izvekova G I, et al. Variability of composition of microbiota of gastrointestinal tract of Perch Perca fluviatilis and Prussian Carp Carassius gibelio during the vegetative season[J]. Journal of Ichthyology, 2021,61:955-971.
[41] Ichikawa S, Abe R, Fujimoto H, et al. Paraburkholderia sabiae administration alters zebrafish anxiety-like behavior via gut microbial taurine metabolism[J]. Frontiers in Microbiology, 2023,14:1079187.
[42] Ren G M, Xu X H, Qu J J, et al. Evaluation of microbial population dynamics in the co-composting of cow manure and rice straw using high throughput sequencing analysis[J]. World Journal of Microbiology and Biotechnology, 2016,32:1-11.
[43] Fang X, Zheng R B, Guo X L, et al. Yak excreta-induced changes in soil microbial communities increased the denitrification rate of marsh soil under warming conditions[J]. Applied Soil Ecology, 2021,165:103935.
[44] Zhang L Y, Li Y, Sun X X, et al. More robust co-occurrence patterns and stronger dispersal limitations of bacterial communities in wet than dry seasons of riparian wetlands[J]. Msystems, 2023,8(2):e01187-22.
[45] Chen J, Zhang B, Wang C, et al. Insight into the enhancement effect of humic acid on microbial degradation of triclosan in anaerobic sediments[J]. Journal of Hazardous Materials, 2024,461:132549.
[46] Mu X X, Shi S Q, Hu X Q, et al. Gut microbiome and antibiotic resistance genes in plateau model animal (Ochotona curzoniae) exhibit a relative stability under cold stress[J]. Journal of Hazardous Materials, 2024,478:135472.
[47] Hernandez D J, David A S, Menges E S, et al. Environmental stress destabilizes microbial networks[J]. The ISME Journal, 2021,15(6):1722-1734.
[48] Zhang L X, Yang Z, Yang F, et al. Gut microbiota of two invasive fishes respond differently to temperature[J]. Frontiers in Microbiology, 2023,14:1087777.
[49] Wu X B, Li X M, Zhu Y J, et al. Effects of water temperature on the growth, antioxidant capacity, and gut Microbiota of Percocypris pingi juveniles[J]. Fishes, 2022,7(6):374.
[50] Turnbaugh P J, Hamady M, Yatsunenko T, et al. A core gut microbiome in obese and lean twins[J]. Nature, 2009,457(7228):480-484.
[51] Ye G L, Dong X H, Yang Q H, et al. Low-gossypol cottonseed protein concentrate used as a replacement of fish meal for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂):Effects on growth performance, immune responses and intestinal microbiota[J]. Aquaculture, 2020,524:735309.
[52] Zhou Y L, He G L, Jin T, et al. High dietary starch impairs intestinal health and microbiota of largemouth bass, Micropterus salmoides[J]. Aquaculture, 2021,534:736261.
[53] Guo K, Zhang R, Luo L, et al. Effects of thermal stress on the antioxidant capacity, blood biochemistry, intestinal microbiota and metabolomic responses of Luciobarbus capito [J]. Antioxidants, 2023, 12(1):198.
[54] Fransen F, Van Beek A A, Borghuis T, et al. Aged gut microbiota contributes to systemical inflammaging after transfer to germ-free mice[J]. Frontiers in Immunology, 2017,8:1385.
[55] Huang P, Cao L P, Du J L, et al. Effects of prometryn exposure on hepatopancreas oxidative stress and intestinal flora in Eriocheir sinensis(Crustacea:Decapoda)[J]. Antioxidants, 2023,12(8):548.