微生物插入序列的多样性及其与功能基因的共现

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Abstract Based on the ISfinder database and reference meta-analysis, this study conducted a systematic study of the diversity of 5812 insertion sequences (ISs) and their co-occurrence with functional genes. The study found significant differences in the distribution of different IS families among hosts, as well as in their co-occurrence with functional genes. DDE-type ISs are predominant, with the IS5 and IS3 families containing the most ISs, while the ISH6 family contains the fewest. ISs are widely found in bacteria and archaea, and several IS families show host specificity, being found only in either bacteria or archaea. The study demonstrated that IS co-occur with various functional genes, such as antibiotic resistance, heavy metal resistance, and stress resistance, indicating their significant role in environmental adaptation and the spread of antibiotic resistance genes among pathogens. Some IS exhibited co-occurrence with multiple functional genes, suggesting broader ecological adaptability, while others showed functional specificity. Future research should focus on experimentally validating the mechanisms through which IS mediate gene transfer and host adaptation, to reveal the mechanism of microbial evolution and ecological adaptation.

Key words： insertion sequence ISfinder database diversity functional genes co-occurrence

Received: 20 October 2024

PACS:	Q933
	X172

Corresponding Authors: 周艳艳,责任作者,助理研究员,yanyanzhou@iue.ac.cn E-mail: yanyanzhou@iue.ac.cn

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	ZHONG Wen-jing
	SU Jian-qiang
	LI Hu
	ZHOU Yan-yan

Cite this article:

ZHONG Wen-jing,SU Jian-qiang,LI Hu等. Diversity of microbial insertion sequences and their co-occurrence with functional genes[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(4): 2314-2322.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I4/2314

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