1. College of Environment, Hohai University, Nanjing 210098, China; 2. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China
Abstract：The adsorption-embedding method was used to prepare immobilized algal-bacteria spheres, in which sodium alginate and activated carbon were used as embedding agents, along with CaCl2 as the cross-linking agent for treating azo dye basic orange ll. The external and internal structures of these spheres were examined via scanning electron microscopy and their reusability was investigated. Results indicate that the optimal preparation conditions were as follows:sodium alginate, activated carbon and CaCl2 concentrations (w/w) of 2.58%, 0.838% and 2%, respectively, an immobilized biomass concentration of 1.139%, a sludge-to-algae mass ratio of 2:1 and an immobilization period of 14h. Decolorization efficiency exceeded 90%, while TOC removal rates ranged from 74% to 90% at influent dye concentrations between 50 and 250mg/L and influent pH values between 6and 10.5. UV-visible full-wavelength and GC-MS scanning spectra demonstrated the destruction of the nitrogen-nitrogen double bond, conjugated system and benzene ring structure in basic orange II, which confirmed the decolorization and degradation reactions, thereby the mineralization of the azo dye. After five cycles of reuse, the algal-bacteria spheres maintained a high efficiency of decolorization and TOC removal.
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