Abstract：To study the changing laws of pollutants under the condition of multi-point confluence of urban sewage pipe network and the mechanism of their influence on microbial reproduction, a set of sewage pipeline pilot system with multiple confluent points was established to explore the migration and transformation characteristics of the three main pollutants of carbon, nitrogen and sulfur in the sewage transportation process. The results showed that the concentration of dissolved chemical oxygen demand (SCOD) and sulfate (SO42-) before the confluence point decreased, the concentration of NH4+-N increased, and the confluence of branch pipes significantly increased the concentration of three types of pollutants at the confluence point. In the later stage, the water quality reached stability. Under the condition that the increase of various pollutants was basically unchanged due to the inlet of branch sewage, the concentration of SCOD decreased from about 320mg/L at the inlet to about 280mg/L at the outlet. The total increase of NH4+-N under the action of ammonification was about 15mg/L, which was higher than the total increase of 12.5mg/L due to the confluences. The results showed that the consumption and metabolism of microorganisms in the confluent pipe network was the dominant factor for the change of carbon and nitrogen pollutants. The concentration of SO42- at the inlet and outlet of the confluent pipe was about 20mg/L, indicating that the confluent pipe and biochemical metabolism maintained the content of SO42- in a state of dynamic equilibrium. In addition, the analysis of the microbial reproduction process in the biological phase of the pipe network pilot system showed that the content of fermenting bacteria (FB), hydrogen-producing acetogen (HPA) and sulfate reducing bacteria (SRB) increased significantly with the propagation time, and the abundance increased at different confluence points along the process. To sum up, the multi-point confluence caused the fluctuation of sewage water quality, which promoted the multiplication and proliferation of microorganisms in the biological phase of the pipe network, and enhanced the dominant position of its metabolism in the transformation of pollutants in the sewage pipe network, making the transformation of pollutants in the pipe network more significant.
Mathioudakis V L, Aivasidis A. Heterotrophic denitrification kinetics in a pressurized sewer biofilm reactor[J]. Desalination, 2009,248(1):696-704.
[2]
张涛,周丹.城市排水管道污水水质的变化过程[J]. 环境科学与技术, 2007,(8):112-114,121. Zhang T, Zhou D. The changing process of sewage water quality in urban drainage pipes[J]. Environmental Science and Technology, 2007,(8):112-114,121.
[3]
金鹏康,焦丁,王宝宝,等.城市污水管网内生物菌群演替规律[J]. 环境科学学报, 2014,34(11):2785-2791. Jin P K, J D, W B B, et al. Succession of biological flora in urban sewage pipe network[J]. Acta Scientiae Circumstantiae, 2014,34(11):2785-2791.
[4]
T H-J, J V, S M J. The sewer as a bioreactor——a dry weather approach[J]. Water science and technology, 2002,45(3):11-24.
[5]
李茂林.基于PIV与FLUENT的排水管道流态研究[D]. 重庆:重庆大学, 2013. Li M L. Research on flow pattern of drainage pipe based on PIV and FLUENT[D]. Chongqing:Chongqing University, 2013.
[6]
Shammay A, Sivret E C, Le-Minh N, et al. Review of odour abatement in sewer networks[J]. Journal of Environmental Chemical Engineering, 2016,4(4,Part A):3866-3881.
[7]
Pai T Y, Shyu G S, Chen L, et al. Modelling transportation and transformation of nitrogen compounds at different influent concentrations in sewer pipe[J]. Applied Mathematical Modelling, 2013,37(3):1553-1563.
[8]
Kadlec R H. Effects of pollutant speciation in treatment wetlands design[J]. Ecological Engineering, 2003,20(1):1-16.
[9]
Liu J, Zhao M, Lv C, et al. The effect of microwave pretreatment on anaerobic co-digestion of sludge and food waste:Performance, kinetics and energy recovery[J]. Environmental Research, 2020,189:109856.
[10]
朱葛夫.厌氧折流板反应器应用技术及微生物群落生态学研究[D]. 哈尔滨:哈尔滨工业大学, 2007. Zhu G F. Application technology of anaerobic baffled reactor and research on microbial community ecology[D]. Harbin:Harbin Institute of Technology, 2007.
[11]
Xu R, Yang Z-H, Zheng Y, et al. Depth-resolved microbial community analyses in the anaerobic co-digester of dewatered sewage sludge with food waste[J]. Bioresource Technology, 2017,244:824-835.
[12]
Guisasola A, Sharma K R, Keller J, et al. Development of a model for assessing methane formation in rising main sewers[J]. Water Research, 2009,43(11):2874-2884.
[13]
Sun J, Ni B-J, Sharma K R, et al. Modelling the long-term effect of wastewater compositions on maximum sulfide and methane production rates of sewer biofilm[J]. Water Research, 2018,129:58-65.
[14]
詹劲基,麦海岸,邱德星,等.一起下水道口如厕硫化氢中毒的调查[J]. 实用预防医学, 2002,9(3):255-255. Zhan J J, Mai H A, Qiu D X, et al. Investigation of hydrogen sulfide poisoning in a toilet at a sewer entrance[J]. Practical Preventive Medicine, 2002,9(3):255-255.
[15]
Pengkang J, Xuan S, Guangxi S, et al. Co-variation between distribution of microbial communities and biological metabolization of organics in urban sewer systems[J]. Environmental Science & Technology, 2018,52(3):1270-1279.
[16]
Zhang Q, Davies E G R, Bolton J R, et al. Impacts of biofilm on monochloramine decay in storm sewer systems:Direct reactions or AOB cometabolism[J]. Biochemical Engineering Journal, 2019,149:107246.
[17]
Li W, Zheng T, Ma Y, et al. Current status and future prospects of sewer biofilms:Their structure, influencing factors, and substance transformations[J]. Science of The Total Environment, 2019,695:133815.
[18]
王宝宝,金鹏康,郝晓宇,等.城市污水管网中污染物转化规律的模拟研究[J]. 中国给水排水, 2015,31(15):24-27. Wang B B, Jin P K, Hao X Y, et al. Simulation study on pollutant transformation law in urban sewage pipe network[J]. China Water & Wastewater, 2015,31(15):24-27.
[19]
孙光溪,金鹏康,宋吉娜,等.城市污水管网中产甲烷菌的分布特性规律[J]. 环境科学, 2016,37(6):2252-2258. Sun G X, Jin P K, Song J N, et al. Distribution characteristics of methanogens in urban sewage pipe network[J]. Environmental Science, 2016,37(6):2252-2258.
[20]
刘鹤蒙,石烜,金鹏康,等.城市污水管网中胞外聚合物的沿程变化特性[J]. 中国给水排水, 2019,35(5):30-35. Liu H M, Shi X, Jin P K, et al. Variation characteristics of extracellular polymer in urban sewage pipe network along the way[J]. China Water & Wastewater, 2019,35(5):30-35.
[21]
Pengkang J, Bin W, Ding J, et al. Characterization of microflora and transformation of organic matters in urban sewer system[J]. Water Research, 2015,84:112-119.
[22]
毕倩.基于肠道微生态调节的制何首乌干预胰岛素抵抗作用及机制研究[D]. 昆明:云南中医学院, 2018. Bi Q. Research on the effect and mechanism of Polygonum multiflorum interfering with insulin resistance based on intestinal microecological regulation[D]. Kunming:Yunnan College of Traditional Chinese Medicine, 2018.
[23]
王宝宝.城市污水管网水质变化特性与生物演替规律研究[D]. 西安:西安建筑科技大学, 2014. Wang B B. Research on water quality change characteristics and biological succession laws of urban sewage pipe network[D]. Xi'an:Xi'an University of Architecture and Technology, 2014.
[24]
Halkjaer N P, Kamma R, Henrik N N, et al. Transformation of wastewater in sewer systems-A review[J]. Water Science and Technology, 1992,25(6).
[25]
杨柯瑶.城市污水管网氮类营养物的迁变规律[D]. 西安建筑科技大学, 2016. Yang K Y. Changes of nitrogen nutrients in urban sewage pipe network[D]. Xi'an University of Architecture and Technology, 2016.
[26]
王斌.城市污水管网微生物群落的演替及其对水质的影响[D]. 西安:西安建筑科技大学, 2015. Wang B. Succession of microbial community in urban sewage pipe network and its impact on water quality[D]. Xi'an:Xi'an University of Architecture and Technology, 2015.
[27]
Sharma K, Derlon N, Hu S, et al. Modeling the pH effect on sulfidogenesis in anaerobic sewer biofilm[J]. Water Research, 2014,49:175-185.
[28]
Dinh N T, Hatta K, Kwon S H, et al. Changes in the microbial community during the acclimation stages of the methane fermentation for the treatment of glycerol[J]. Biomass and Bioenergy, 2014,68:240-249.
[29]
Yassin A F, Inglis T J J, Hupfer H, et al. Cruoricaptor ignavus gen. nov., sp. nov., a novel bacterium of the family Flavobacteriaceae isolated from blood culture of a man with bacteraemia[J]. Systematic and Applied Microbiology, 2012,35(7):421-426.
[30]
Shun'ichi I, Shino S, M N-K T, et al. Microbial population and functional dynamics associated with surface potential and carbon metabolism[J]. The ISME Journal, 2014,8(5):963-978.
[31]
Rogosa M. The Genus veillonella. I. General cultural, ecological, and biochemical considerations[J]. Journal of Bacteriology, 1964,87:162-170.
[32]
Yang Q, Xiong P, Ding P, et al. Treatment of petrochemical wastewater by microaerobic hydrolysis and anoxic/oxic processes and analysis of bacterial diversity[J]. Bioresource Technology, 2015,196:169-175.
[33]
James H, M W L, A P L, et al. Draft genome sequence of Levilinea saccharolytica KIBI-1, a member of the Chloroflexi Class Anaerolineae[J]. Genome announcements, 2015,3(6).
[34]
Dastidar A, Wang Y-T. Modeling arsenite oxidation by chemoautotrophic Thiomonas arsenivorans strain b6in a packed-bed bioreactor[J]. Science of the Total Environment, 2012,432:113-121.
[35]
Bilen M, Cadoret F, Daoud Z, et al. Parabacteroides timonensis sp. nov., identified in human stool[J]. Human Microbiome Journal, 2016,2:1-2.
[36]
Taylor J, Parkes R J. The Cellular fatty acids of the sulphate-reducing bacteria, desulfobacter sp., Desulfobulbus sp. and Desulfovibrio desulfuricans[J]. Journal of General Microbiology, 1983,129(11):3303-3309.
[37]
Fukui M, Teske A, Aßmus B, et al. Physiology, phylogenetic relationships, and ecology of filamentous sulfate-reducing bacteria (genus Desulfonema)[J]. Archives of Microbiology, 1999,172(4):193-203.
[38]
Tomoko M, Atsushi K, Takashi A, et al. Complete genome sequence of Acidithiobacillus ferridurans JCM 18981[J]. Microbiology Resource Announcements, 2018,7(7).
[39]
杨雷.污水土地处理工艺处理有机废水的研究[D]. 青岛:青岛理工大学, 2010. Yang L. Research on the treatment of organic wastewater by sewage land treatment process[D]. Qingdao:Qingdao Technological University, 2010.
[40]
Cao J, Zhang L, Hong J, et al. Different ferric dosing strategies could result in different control mechanisms of sulfide and methane production in sediments of gravity sewers[J]. Water Research, 2019, 164:114914.
