The inactivation efficiency of ultrasound combined with UV-LED on tetracycline-resistant Escherichia coli

LI Song-wei; ZHOU Xiao-qin; ZHAO Mei-juan; LI Zi-fu; XIA De-hua

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China Environmental Science ›› 2023, Vol. 43 ›› Issue (12) : 6313-6320.

Water Pollution Control

The inactivation efficiency of ultrasound combined with UV-LED on tetracycline-resistant Escherichia coli

LI Song-wei¹, ZHOU Xiao-qin¹, ZHAO Mei-juan¹, LI Zi-fu¹, XIA De-hua²

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Abstract

In this study, the inactivation efficiency as well as membrane damage of tetracycline-resistant Escherichia coli under ultrasound at different frequencies (33, 120, 200kHz) and different UV-LED wavelengths (255, 275nm) were evaluated respectively, and furthermore, the optimized operation parameter were selected to form combined ultrasound and UV-LED process to control ARB in sewage effectively. The results showed that 33 kHz ultrasound alone was effective in ARB inactivation, and with an input power of 50 W accumulated to an input dose of 1080 KJ/L, the inactivation rate of Escherichia coli can reach to 2.30 log, while the percentage of cell membrane damage was 61.09%. For UV-LED alone, when 255nm UV-LED irradiated at dosage of 19.92mJ/cm², the inactivation rate of bacteria and the percentage of cell membrane damage were 5.32log and 2.52% respectively. While the inactivation rate and the percentage of cell membrane damage were 4.63 log and 34.95% respectively for 275nm UV-LED irradiation, but dual-wavelength UV-LED irradiation did not show synergistic effect in bacteria inactivation. However, ultrasound combined with UV-LED can obviously improve the disinfection efficiency, the inactivation rate increased to be 7.45log, the cell membrane damage increased to be 68.74%. Meanwhile, the photoreactivation rate of bacteria after disinfection was reduced to 0.4%. The combination of ultrasound and UV-LED shows great potential in ARB control during wastewater treatment.

Key words

inactivation efficiency / photoreactivation / resistant bacteria / ultrasound / UV-LED

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LI Song-wei, ZHOU Xiao-qin, ZHAO Mei-juan, LI Zi-fu, XIA De-hua. The inactivation efficiency of ultrasound combined with UV-LED on tetracycline-resistant Escherichia coli[J]. China Environmental Science. 2023, 43(12): 6313-6320

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