Size effect of polyethylene terephthalate microplastics on Anammox granular sludge

HONG Xian-tao; ZHOU Xin

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China Environmental Science ›› 2023, Vol. 43 ›› Issue (12) : 6406-6412.

Water Pollution Control

Size effect of polyethylene terephthalate microplastics on Anammox granular sludge

HONG Xian-tao^1,2, ZHOU Xin^1,2

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Abstract

To investigate the size-dependent effect of microplastics (MPs) on nitrogen removal performance of activated sludge, the effect of polyethylene terephthalate (PET) on anaerobic ammonia-oxidizing granular sludge (AnGS) at different particle sizes (75~500μm) was explored using the methods of water quality determination, activity test, physicochemical characterization and microbial community analysis in this study. Results showed that the nitrogen removal efficiency of Anammox was lower than that of the control group when 75 and 150μm PET-MPs were dosed, while the 300 and 500μm groups showed superiority over the control group. Anaerobic ammonia oxidation activity (SAA) tests showed similar results. Extracellular polymeric substances (EPS) analysis revealed that the smaller the particle size of PET-MPs, the higher the EPS secretion and PN/PS, and the more incomplete the AnGS structure. The inhibition of Anammox by PET at small particle size was mainly associated with the granular sludge's lower Zeta potential and smaller specific surface area. In contrast, large-size PET-MPs reduced the chance of contact with MPs and provided more active sites, facilitating Anammox substrates' delivery efficiency. High-throughput sequencing results showed that the particle size of PET-MPs determined the diversity and abundance of microbial community, especially the relative abundance of Candidatus Kuenenia.

Key words

anammox / biological nitrogen removal / effect / microbial community / microplastics / PET

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HONG Xian-tao, ZHOU Xin. Size effect of polyethylene terephthalate microplastics on Anammox granular sludge[J]. China Environmental Science. 2023, 43(12): 6406-6412

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