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| Refined imaging of pH and O2 across the passivated soil-root micro-interfaces |
| REN Jing-hua1, FAN Jian1, SUN Yu2, LIAO Qi-lin1, XU Wei-wei1, LIULing1, HAN Chao2, GU Xue-yuan3 |
arable, Ministry of Natural Resources, Geological Survey of Jiangsu Province, Nanjing 210018, China;
2. State Key Laboratory of Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;
3. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China |
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Abstract Herein two self-established planar optode methods (PO) were successfully deployed to in situ, high-resolution investigate the fine-scale and simultaneous variations of micro-environmental conditions e.g. pH and O2 across the passivated soil-root interfaces. For the first time, our results quantitatively and directly revealed the high degrees of spatiotemporal heterogeneity of pH and O2 throughout the rhizosphere at the sub-mm scale. Significant differences (P<0.01) among three Cd treatments were observed. The addition of the attapulgite clays significant elevated the pH micro-environemnts, but had slight effects on the micro-distributions of O2 within the soil profiles. There was a visible and distinct range of 2~5mm surrounding the rice roots where pH values were generally decreased by 0.31~0.87 and O2 concentrations were increased to 58.56~82.01μmol/L. Notably higher rhizosphere acidification and O2 leakage always occurred locally around the basal roots. The higher abilities of rhizosphere acidification and O2 leakage within the passivated soil-root micro interfaces would potentially promote the reactivation and root uptake of passivated Cd.
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Received: 24 August 2022
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