<i>Klebisella</i>还原合成纳米钯的电子传递途径解析

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Abstract Chem-Pd (chemically synthesized palladium nanoparticle) and Bio-Pd (biosynthetic palladium nanoparticle) were prepared using chemical and biological methods, respectively, with sodium formate serving as the electron donor. The TEM and XRD results revealed that the Chem-Pd was agglomerated with an average particle size of 12nm, while Bio-Pd was evenly distributed inside the cell and in the extracellular polymeric substance (EPS), with an average size of 5nm. The reduction curve of bivalent palladium showed that the rate of bivalent palladium reduction by the biological method was 70% higher than that by the chemical method, indicating that the intervention of microorganisms can effectively promote the formation of nano palladium. By exploring the effect of intracellular electron transfer and extracellular polymer on the synthesis of Bio-Pd, the synthesis mechanism of Bio-Pd was clarified: the Klebsiella oxytoca strain transferred electrons to extracellular palladium divalent via its respiratory chain. Meanwhile, the presence of redox active groups in EPS may enhance Bio-Pd production outside the cell. Additionally, the test of live/dead bacteria showed that Bio-Pd remained harmful to cells.

Key words： Klebsiella strain nano-palladium extracellular electron transfer respiratory chain inhibitors

Received: 10 December 2022

PACS:

X781.1

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	Articles by authors
	JU Cheng-jia
	WANG Zi-yang
	CAO Wen-ying
	YU Lei
	QIU Xu-hai
	ZHOU Shao-hua
	WANG Quan
	CHEN Rong-ping

Cite this article:

JU Cheng-jia,WANG Zi-yang,CAO Wen-ying等. The electron transfer research of biogenic nano-palladium by Klebsiella strain[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3554-3561.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/I7/3554

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