石油降解菌的筛选鉴定及混合菌系的构建

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Abstract The enrichment and separation method was used to select oil-degrading bacteria from the soil around an oil refinery in northern Shaanxi of China. The identification of bacterial strains was performed by physiological and biochemical assays and 16SrDNA gene sequence. Then the parameters such as pH value, salinity, nitrogen source, inoculum size and oil concentration were selected as single factors on selected strains growth. Besides, through antibiotic tests, oil-degrading bacterial consortium was constructed with the selected strains for oil treatment. Four oil-tolerance bacterial strains including Acinetobacter (T2、T4、T5) and Bacillus (T3) were selected; pH value, salinity, nitrogen source, inoculum size and oil concentration affected the growth of the four strains. Among 15 bacterial combinations without inhibiting effect on each other between the four strains, consortium P mixed with a volume ratio as 1:1:1:1 showed the highest oil degradation rate of 89% in the same time. This paper provides a theoretical reference for microbial remediation of oil-contaminated soil.

Key words： oil degradation bacteria 16SrDNA antagonistic experiments bacterial consortium oil-degradation rate

Received: 11 April 2023

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X172

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	JIA Miao-miao
	LI Hai-hong
	DENG Tian-tian

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JIA Miao-miao,LI Hai-hong,DENG Tian-tian. Isolation, identification of oil degrading bacteria and construction of bacterial consortium[J]. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(S1): 213-220.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2023/V43/IS1/213

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