三氯生和双酚A对斑马鱼神经毒性的比较研究

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1795 KB) HTML (0 KB)
Export: BibTeX | EndNote (RIS)

Abstract In this research, two typical environmental endocrine disruptors, triclosan(TCS) and bisphenol A(BPA) were selected as target chemicals and zebrafish (Danio rerio) were used as vertebrate model organisms. The effects of TCS and BPA on zebrafish neurodevelopment and locomotor behavior were investigated in detail. Results showed that both TCS and BPA induced phenotypic malformations in zebrafish embryos, such as pericardial edema, yolk cysts and swiming sac closure. Besides, their exposure inhibited the locomotor activity, damaged motor-related neurons and affected the activity of acetylcholinesterase in larval zebrafish, resulting in neurobehavioral disorders. Moreover, TCS and BPA both led to a decrease in the number of neonatal neuronal cells and an increase in apoptotic cells in zebrafish brain, which had a severe impact on the development of central nervous system. Target prediction combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) cluster analysis was used to compare the similarities and differences between the metabolic pathways of TCS and BPA, as well as their underlying toxicity mechanisms. These findings provide an important theroretical reference for risk assessment and early warning of environmental exposure to TCS and BPA.

Key words： triclosan bisphenol A zebrafish neurotoxicity pathogenic mechanisms emerging pollutants

Received: 30 June 2023

PACS:

X174

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HAN Xiao-wen
	XU Jie-yu
	WANG Wei-wei
	QIAN Qiu-hui
	WANG Hui-li

Cite this article:

HAN Xiao-wen,XU Jie-yu,WANG Wei-wei等. Effects of triclosan and bisphenol A on zebrafish neurotoxicity[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(2): 1111-1121.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I2/1111

[1]	Li M, He Y, Sun J, et al. Chronic exposure to an environmentally relevant triclosan concentration induces persistent triclosan resistance but reversible antibiotic tolerance in Escherichia coli [J]. Environmental Science & Technology, 2019,53(6):3277-3286.
[2]	Liu X, Tu M, Wang S, et al. Research on freshwater water quality criteria, sediment quality criteria and ecological risk assessment of triclosan in China [J]. Science of the Total Environment, 2022,816:151616.
[3]	Lalonde B, Garron C, Dove A, et al. Investigation of spatial distributions and temporal trends of triclosan in Canadian surface waters [J]. Archives of Environmental Contamination and Toxicology, 2019,76:231-245.
[4]	Liang Y, Song H, Wu Y, et al. Occurrence and distribution of triclosan and its transformation products in Taihu Lake, China [J]. Environmental Science and Pollution Research, 2022,29(56):84787-84797.
[5]	Montaseri H, Forbes P B C. A review of monitoring methods for triclosan and its occurrence in aquatic environments [J]. Trac-Trends in Analytical Chemistry, 2016,85:221-231.
[6]	Wang M, Hu B, Zhou W, et al. Enhanced hand-to-mouth exposure from hand sanitizers during the COVID-19pandemic:a case study of triclosan [J]. Sci. Bullet, 2022,67:995-998.
[7]	Sun C, Zhang T, Zhou Y, et al. Triclosan and related compounds in the environment:Recent updates on sources, fates, distribution, analytical extraction, analysis, and removal techniques [J]. Science of the Total Environment, 2023,870:161885.
[8]	Bai X, Zhang B, He Y, et al. Triclosan and triclocarbon in maternal-fetal serum, urine, and amniotic fluid samples and their implication for prenatal exposure [J]. Environmental Pollution, 2020,266:115117.
[9]	Czarny-Krzyminska K, Krawczyk B, Szczukocki D. Bisphenol A and its substitutes in the aquatic environment:Occurrence and toxicity assessment [J]. Chemosphere, 2023,315:137763.
[10]	牛宇庆.典型环境污染物BPA、BaP对斑马鱼胚胎的毒性效应研究[D]. 太原:山西大学, 2020. Niu Y Q. Toxic effects of typical environmental pollutants BPA and BaP on zebrafish embryos [D]. Taiyuan:Shanxi University, 2020.
[11]	Liu Y, Wu Y, Qin G, et al. Bioaccumulation and reproductive toxicity of bisphenol A in male-pregnant seahorse (Hippocampus erectus) at environmentally relevant concentrations [J]. Science of the Total Environment, 2021,753:141805.
[12]	Zhao J-L, Zhang Q-Q, CHEN F, et al. Evaluation of triclosan and triclocarban at river basin scale using monitoring and modeling tools:Implications for controlling of urban domestic sewage discharge [J]. Water Research, 2013,47(1):395-405.
[13]	郭子一,王伟伟,宋杰,等.基于Illumina RNA-Seq分析的三种内分泌干扰物对斑马鱼神经毒性效应与机制[J]. 中国环境科学, 2023, 43(2):946-956. Guo Z Y, Wang W W, Song J, et al. Neurotoxicity effects of the three endocrine disruptors on zebrafish and the underlying molecular mechanisms by using the Illumina RNA-seq technique [J]. China Environmental Science, 2023,43(2):946-956.
[14]	赵晨曦,王杨,钱秋慧,等.三氯卡班环境暴露对斑马鱼神经行为的影响[J]. 中国环境科学, 2022,42(1):456-564. Zhao C X, Wang Y, Qian Q H, et al. Effects of environmental exposure to triclocarban on the neurobehavior of zebrafish (Danio rerio) [J]. China Environmental Science, 2022,42(1):456-564.
[15]	郭弘扬,唐天乐,朱晓鹏.斑马鱼模型在中枢神经系统疾病中的研究进展[J]. 中国海洋药物, 2020,39(3):71-79. Guo H Y, Tang T L, Zhu X P. Research progress of danio rerio model in central nervous system diseases [J]. Chinese Journal of Marine Drugs, 2020,39(3):71-79.
[16]	Ogawa Y, Kakumoto K, Yoshida T, et al. Elavl3is essential for the maintenance of Purkinje neuron axons [J]. Scientific Reports, 2018,8(1):2722.
[17]	Nakamura K, Taniguchi T, Hirabayashi M, et al. Altered properties of endothelial cells and mesenchymal stem cells underlying the development of scleroderma-like vasculopathy in KLF5(+/-); Fli-1(+/-) mice [J]. Arthritis & Rheumatology, 2020,72(12):2136-2146.
[18]	Liu X, Wang Z, Zhang X, et al. LncRNA MEG3activates CDH2expression by recruitment of EP300 in valproic acid-induced autism spectrum disorder [J]. Neuroscience Letters, 2022,783:136726.
[19]	Shimizu Y, Kawasaki T. Histone acetyltransferase EP300regulates the proliferation and differentiation of neural stem cells during adult neurogenesis and regenerative neurogenesis in the zebrafish optic tectum [J]. Neuroscience Letters, 2021,756:135978.
[20]	Nwilson K M, Abbruzzesse G, Kenyon K, et al. Pa2G4is a novel Sixl co-factor that is required for neural crest and otic development [J]. Developmental Biology, 2017,421(2):171-182.
[21]	Ueno K, Koga T, Kato K, et al. MUC1mucin is a negative regulator of Toll-like receptor signaling [J]. American Journal of Respiratory Cell and Molecular Biology, 2008,38(3):263-268.
[22]	Fan S, Weixuan W, Han H, et al. Role of NF-Kappa B in lead exposure-induced activation of astrocytes based on bioinformatics analysis of hippocampal proteomics [J]. Chemico-Biological Interactions, 2023,370:110310.
[23]	Kalueff A V, Echevarria D J, Homechaudhuri S, et al. Zebrafish neurobehavioral phenomics for aquatic neuropharmacology and toxicology research [J]. Aquatic Toxicology, 2016,170:297-309.
[24]	Sun L, Ling Y, Jiang J, et al. Differential mechanisms regarding triclosan vs. bisphenol A and fluorene-9-bisphenol induced zebrafish lipid-metabolism disorders by RNA-Seq [J]. Chemosphere, 2020, 251:126318.
[25]	Ling Y, Sun L, Wang D, et al. Triclosan induces zebrafish neurotoxicity by abnormal expression of miR-219targeting oligodendrocyte differentiation of central nervous system [J]. Archives of Toxicology, 2020,94(3):857-871.
[26]	Heredia-Garcla G, Elizalde-Velazquez G A, Gomez-Olivan L M, et al. Realistic concentrations of Bisphenol-A trigger a neurotoxic response in the brain of zebrafish:Oxidative stress, behavioral impairment, acetylcholinesterase inhibition, and gene expression disruption [J]. Chemosphere, 2023,330:138729.
[27]	Pulaguri N, Grover P, Abhishek S, et al. Triclosan affects motor function in zebrafish larva by inhibiting ache and syn2a genes [J]. Chemosphere, 2021,266:128930.
[28]	Wang W W, Li X, Qian Q H, et al. Mechanistic exploration on neurodevelopmental toxicity induced by upregulation of alkbh5targeted by triclosan exposure to larval zebrafish [J]. Journal of Hazardous Materials, 2023,457:131831.
[29]	Augustin H G, Koh G Y. Organotypic vasculature:From descriptive heterogeneity to functional pathophysiology [J]. Science, 2017,357(6353):eaal2379.
[30]	Szychowski K A, Wnuk A, Kajta M, et al. Triclosan activates aryl hydrocarbon receptor (AhR)-dependent apoptosis and affects Cyp1a1and Cyp1b1expression in mouse neocortical neurons [J]. Environmental Research, 2016,151:106-114.
[31]	Qi S, Fu W, Wang C, et al. BPA-induced apoptosis of rat Sertoli cells through Fas/FasL and JNKs/p38MAPK pathways [J]. Reproductive Toxicology, 2014,50:108-116.
[32]	Gyimah E, Xu H, Dong X, et al. Developmental neurotoxicity of low concentrations of bisphenol A and S exposure in zebrafish [J]. Chemosphere, 2021,262:128045.
[33]	Kim J H, Kim K, Kim I, et al. Overexpression of neurogenin 1negatively regulates osteoclast and osteoblast differentiation [J]. International Journal of Molecular Sciences, 2022,23(12):6780.
[34]	Huang J, Huang W, Zhou R, et al. Detection and significance of glial fibrillary acidic protein antibody in autoimmune astocytopathy and related diseases [J]. Annals of translational medicine, 2023,11(7):288.
[35]	Xiao L, Tang X, Hu X, et al. Serum level of growth-associated protein 43Is associated with first-episode schizophrenia patients without antipsychotic drugs treatment [J]. Computational Intelligence and Neuroscience, 2022,2022:4719271.
[36]	Moyano P, Flores A, Garcia J, et al. Bisphenol A single and repeated treatment increases HDAC2, leading to cholinergic neurotransmission dysfunction and SN56cholinergic apoptotic cell death through AChE variants overexpression and NGF/TrkA/P75NTR signaling disruption [J]. Food and Chemical Toxicology, 2021,157:112614.
[37]	Murugan R, Haridevamuthu B, Kumar R S, et al. Deacetyl epoxyazadiradione ameliorates BPA-induced neurotoxicity by mitigating ROS and inflammatory markers in N9cells and zebrafish larvae [J]. Comparative biochemistry and physiology Toxicology & pharmacology:CBP, 2023,271:109692.
[38]	高月虹,刘梦茹,杨宪鑫,等.芳香烃受体通过p38MAPK/p65NF-κB信号通路调节绿脓菌素诱导的巨噬细胞中炎症因子的表达[J]. 中国药理学通报, 2023,(7):1296-1302. Gao Y H, Liu M R, Yang X X, et al. Aromatic hydrocarbon receptors are regulated by the p38MAPK/p65NF κB signaling pathway Expression of inflammatory cytokines in pyocyanin-induced macrophages [J]. Chinese Pharmacological Bulletin, 2023,(7):1296-1302.
[39]	Szychowaki K A, Skora B, Wojtowicz A K. Triclosan affects the expression of nitric oxide synthases (NOSs), peroxisome proliferator-activated receptor gamma (PPARγ), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in mouse neocortical neurons in vitro [J]. Toxicology in Vitro, 2021,73:105143.
[40]	Szychowski K A, Skora B, Wojtowocz A K. Involvement of sirtuins (Sirt1and Sirt3) and aryl hydrocarbon receptor (AhR) in the effects of triclosan (TCS) on production of neurosteroids in primary mouse cortical neurons cultures [J]. Pesticide Biochemistry and Physiology, 2022,184:105131.
[41]	王欣.全氟辛烷磺酸(PFOS)对斑马鱼幼鱼运动行为和Wnt通路及神经肌肉相关基因表达的影响[D]. 汕头:汕头大学, 2022. Wang X. Perfluorooctane sulfonic acid (PFOS) exposures interfere behaviors and transcriptions of genes on Wnt signaling pathway and neuromuscular system in zebrafish larvae [D]. Shantou:Shantou University, 2022.
[42]	周继勤.神经干细胞分泌组通过激活PPAR-γ调节小胶质细胞表型抑制脂多糖诱导的神经炎症[D]. 镇江:江苏大学, 2022. Zhou J Q. Neural stem cell secretom inhibits lipopolysaccharide-induced neuroinflammation through modulating microglia phenotypes by activating PPAR-γ [D]. Zhenjiang:Jiangsu University, 2022.
[43]	李丽.基于色氨酸代谢通路探讨中药改善肾虚血瘀型EMs性不孕症IVF结局的机制[D]. 济南:山东中医药大学, 2022. Li L. Study on the mechanism of traditional Chinese medicine improving IVF outcome of endometriosis-related infertility with kidney deficiency and blood stasis type based on tryptophan metabolism pathway [D]. Jinan:Shandong University of Traditional Chinese Medicine, 2022.