典型抗生素冲击对黄铁矿/硫基质反硝化修复体系的影响

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Abstract In order to evaluate the performance of denitrification process in the pyrite/sulfur-based remediation system (FeS₂/S⁰) under different types of typical antibiotics, sulfonamides (sulfamethoxazole (SMZ) and sulfadiazine (SDZ)), fluoroquinolones (enrofloxacin (ENR) and ofloxacin (OFL)), tetracycline (tetracycline (TCY) and oxytetracycline (OTC)), macrolides (erythromycin (ERY) and spiramycin (SPM)) which are usually highly detected in groundwater were taken as typical antibiotics with a set range of concentrations from 100ng/L to 500ug/L. Based on the simulated groundwater microcosm experimental system in this study, the changes in nitrogen, nitrate reductase (NAR), nitrite reductase (NIR) and electron transfer activity (ETSA) during the denitrification in the FeS₂/S⁰ remediation system were investigated. The results show that denitrifying inhibition effect was significantly associated with concentration and species of antibiotic at the order of inhibition degree as:OFL (5.81%~27.73%) > ENR (3.06%~14.17%), OTC (1.95%~14.25%) > SMZ (2.54%~11.75%), SDZ (1.87%~10.90%) > TCY (1.98%~9.44%), SPM (2.45%~9.43%) > ERY (2.13%~8.47%), and the higher the concentration, the greater the level of inhibition. In all experimental groups, the removal rate of NO₂^--N was lower than that of NO₃^--N, the activity of both NAR and NIR increased and then decreased, resulting in a single peak change with the greatest reduction. There was a positive correlation of the removal rate of NO₃^--N to the concentrations of SO₄^2--S, TFe and Fe²⁺ in effluent. The most significant denitrification inhibition occurred in OFL interrupting groups. Contrasting to the control group, the average value of ETSA in each experimental system (especially in the OFL stressing group) decreased. Meanwhile, the k value in each reaction system of antibiotics did not change much at the ng/L level but varied significantly at the mg/L concentration level, and the k value was significantly lower in the OFL reaction system than in other reaction systems.

Key words： groundwater nitrate denitrification antibiotics enzyme sensitivity

Received: 25 June 2023

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X523

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	CAO Xi-shuang
	XIN Xin
	YANG Wen-yu
	LIU Xin
	PAN Xian-bing

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CAO Xi-shuang,XIN Xin,YANG Wen-yu等. Effects of typical antibiotics on denitrifying performance in the pyrite/sulfur-based remediation system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(2): 793-802.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I2/793

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