介质阻挡放电联合脉冲电晕放电灭活水中维氏气单胞菌的效果与机制

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Abstract In this study, the DBD combined with PCD (DBD/PCD) was used to inactivate Aeromonas veronii in water. The synergistic effect of DBD and PCD was analyzed, and the effects of voltage, pH, carrier gas type and flow rate on the inactivation efficiency of Aeromonas veronii were investigated. The inactivation mechanism of Aeromonas veronii was explored, The types and effects of active substances were analyzed, and the safety of the DBD/PCD was verified through the experiment of zebrafish challenge. The results showed that the inactivation effect of DBD/PCD to Aeromonas veronii was significantly higher than that of DBD and PCD alone, and their synergistic coefficients were both greater than 1. After 420s of treatment, DBD/PCD can reduce the concentration of Aeromonas veronii by 7 orders of magnitude; Increasing the discharge voltage can significantly improve the inactivation efficiency of Aeromonas veronii; Under alkaline conditions, oxygen as a carrier gas is beneficial for the inactivation of Aeromonas veronii; The effect of air flow rate on inactivation efficiency is not obvious in the range of 0.5~1.5L/min. The active substances produced in the DBD/PCD system first act on the outer membrane of Aeromonas veronii cells, causing them to rupture, and then enter the cells to cause damage to intracellular proteins and the virulence genes, ultimately leading to the inactivation of Aeromonas veronii. Active substances (·OH, ¹O₂ and O₂^-) produced by DBD/PCD system play an important role in the inactivation of Aeromonas veronii. The experiment of zebrafish challenge showed that the DBD/PCD is a safe and efficient method to inactivate pathogenic microorganisms in water bodies.

Key words： dielectric barrier discharge pulse corona discharge Aeromonas veronii inactivation mechanism zebrafish

Received: 11 February 2024

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X172

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	ZHU Zhen-fei
	YAN Peng-cheng
	ZHANG Rong
	ZHANG Min
	QU Guang-zhou

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ZHU Zhen-fei,YAN Peng-cheng,ZHANG Rong等. The effect and mechanism of dielectric barrier discharge combined with pulse corona discharge to inactivate of Aeromonas veronii in water[J]. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(9): 4968-4977.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2024/V44/I9/4968

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