EPD系统对不同类别小分子量PAHs的响应分析

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Abstract In order to investigate the tolerance of endogenous partial denitrification (EPD) system to different types of low molecular weight polycyclic aromatic hydrocarbons (PAHs) and to explore methods to enhance the impact resistance of EPD systems, this study first acclimated EPD systems with 20mg/L PAHs (phenanthrene and anthracene), and then added other types of PAHs (anthracene, phenanthrene, and naphthalene) at concentrations of 0~80mg/L to the EPD system to analyze the mechanisms of PAHs tolerance by batch tests. The results indicated that under the stress of phenanthrene and anthracene, the EPD systems maintained a high accumulation rate of 86% for NO₂^--N and a removal capacity of 50% for PO₄^3--P. In the anthracene system, the microorganisms secreted more extracellular polymeric substances to protect themselves, while a greater enrichment of PAH-RHD GNF/R and PAH-RHD GPF/R genes was observed to enhance tolerance to PAHs in the phenanthrene system. The introduction of phenanthrene and anthracene significantly enriched denitrifying glycan bacteria and denitrifying phosphorus accumulating bacteria. The denitrifying activity of the EPD system acclimated with phenanthrene was (167.429±2.321)mgN/(gVSS×h), and it still maintained a well phosphorus removal capacity under the stress of naphthalene and anthracene. The EPD system acclimated with anthracene maintained high NO₂^--N accumulation capacity under the stress of naphthalene and phenanthrene, with denitrifying bacterial activity at (220.137±0.575)mgN/(gVSS×h). This study provides the theoretical support for the tolerance of EPD systems to low molecular weight PAHs and also proposes insights into enhancing the impact resistance of EPS system through technological interventions, which has significant importance for optimizing the operational effectiveness of EPD in wastewater treatment.

Key words： endogenous partial denitrification polycyclic aromatic hydrocarbons tolerance functional genes microbial community structure denitrifying bacteria activity

Received: 05 August 2024

PACS:

X703

Corresponding Authors: 吉建涛,教授,jijiantao999@zzu.edu.cn;金宝丹,副教授,2016024@zzuli.edu.cn E-mail: jijiantao999@zzu.edu.cn;2016024@zzuli.edu.cn

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	BAI Zhi-xuan
	YAN Ye-yu
	JI Jian-tao
	JIN Bao-dan
	LIU Ye
	DU Jing-jing
	WANG Lan

Cite this article:

BAI Zhi-xuan,YAN Ye-yu,JI Jian-tao等. Response analysis of endogenous partial denitrification system to different categories of low molecular weight PAHs[J]. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(3): 1241-1250.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2025/V45/I3/1241

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