内蒙古河套灌区不同盐碱程度的土壤细菌群落多样性

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Abstract

Denaturing gradient gel electrophoresis (DGGE) based on analyzing V3~V6 variable regions of bacterial 16S rDNA, were used to examine the microbial community structure and diversity in different saline-alkaline soils (saline soil, strongly salinized soil and slight salinized soil) and soil depths (0~20cm, 20~30cm) in the Hetao Area of Inner Mongolia. Their physical and chemical properties were measured, respectively. Experimental results showed that bacterial community structure and diversity decreased with the different saline-alkaline soil (slight salinized soil > strongly salinized soil > saline soil)and declined with soil depths (0~20cm > 20~30cm). Shannon-Wiener index of bacterial community in slight salinized soil was the highest value (3.36), while it was only 3.05 and 2.49 in strongly salinized soil and saline soil. The cluster analysis of DGGE bands showed that the bacterial community structure and diversity formed two distinct clusters 0~20cm and 20~30cm. Shannon-Wiener index of bacterial community in 0~20cm layer (saline soil 3.04, strongly salinized soil 3.29, slight salinized soil 3.36) were higher than 20~30cm layer (saline soil2.49, strongly salinized soil 3.05, slight salinized soil 3.14). Analyses of Pearson correlation and CCA revealed that variations of bacterial community structure were mainly affected by contents of w(EC), pH, w(SOC), w(TP). The soil bacteria diversification index has a negative correlation with the w(EC)(r=-0.542,P < 0.05)、pH(r=-0.526,P < 0.05), and bacterial community diversity exhibited very significant positive correlation with w(SOC)(r=0.700,P < 0.01) and w(TP)(r=0.805,P < 0.01). w(EC) and pH were the the greatest influence in saline-alkaline soils. Twenty bands were excised from the DGGE gel and re-amplified for 16S rDNA sequencing. Based on the sequencing results, eleven bands can be identified as related to Proteobacteria. These results provide evidence that Proteobacteria are the domain bacterial communities in different saline-alkaline soils of Hetao Area of Inner Mongolia. Saline-alkali soils were mostly bacterial strains belonging to tolerant salty.

Key words： Hetao Area saline-alkaline soils bacterial DGGE (denaturing gradient gel electrophoresis) principal component analysis

Received: 18 May 2015

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X172

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	Articles by authors
	LI Xin
	JIAO Yan
	DAI Gang
	YANG Ming-de
	WEN Hui-yang

Cite this article:

LI Xin,JIAO Yan,DAI Gang等. Soil bacterial community diversity under different degrees of saline-alkaline in the Hetao Area of Inner Mongolia[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(1): 249-260.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I1/249

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