Cl-、SO42-和PO43-对异养反硝化污泥的胁迫效应

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Abstract

As the functional part of denitrification, denitrifier was obviously stressed by anions. To investigate the effects and the mechanism of anions on heterotrophic denitrifying sludge(HDS) are of great significance for the practical application of high-rate denitrification in saline nitrogen-rich wastewater treatments. Nitrate reductase and alkaline phosphatase were chosen as the indices to determine the effects of Cl^-, SO₄^2- and PO₄^3- on the enzyme activities in HDS, while the ratios of live/dead cells and cellular morphology were examined and observed to characterise the effects of Cl^-, SO₄^2- and PO₄^3- on the cell structures in HDS. Results showed that, the IC50values of Cl^-, SO₄^2- and PO₄^3- for nitrate reductase were 0.15, 0.12 and 0.05mol/L respectively, while the IC50values of Cl^-, SO₄^2- and PO₄^3- for alkaline phosphatase were 1.14, 0.75 and 0.49mol/L, respectively. Anions with high concentrations(1.71mol/L Cl^-, 0.85mol/L SO₄^2-, 0.57mol/L PO₄^3-) resulted in the damage of cell membranes, and caused the leakage of cell inclusion. In conclusion, the effects of anions on HDS were attributed to the osmotic stresses which affected the enzyme activities, and the ion stresses which caused the damage of cell membranes.

Key words： anion heterotrophic denitrifying sludge osmotic stresses ion stresses

Received: 23 September 2015

PACS:

X703

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	Articles by authors
	WANG Ru
	ZHANG Ping
	YANG Cheng
	XU Shao-yi
	DAI Chen-lin
	Abbas Ghulam

Cite this article:

WANG Ru,ZHANG Ping,YANG Cheng等. Stress of Cl^-, SO₄^2- and PO₄^3- on the heterotrophic denitrifying sludge[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(4): 1039-1044.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I4/1039

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