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| The role and microbial interaction of anammox in WWTPs with AAO process |
| WANG Shan-yun1, MA Bin2, JIA Fang-xu2, PENG Yong-zhen1,2 |
1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;
2. Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China |
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Abstract Basing on qPCR assay and 15N isotope tracing technique, the abundance, rate, role, and microbial interaction of anammox was investigated in three WWTPs of anaerobic-anoxic-oxic (AAO) process. Results showed anammox bacteria was detected in all samples with abundance of 106~107copies/g VSS, and rate of 0.11~0.90 μmol N/(g VSS·h). As for the microbial interaction among anammox and other microbial process, heterotrophic denitrification was not only a more important provider but a stronger competitor for NO2-, compared with autotrophic nitrification, in which AOB was the major NO2- producer. The roles of anammox to ammonia oxidation and nitrogen removal were calculated to be 2.55%~7.89% and 2.07%~6.59%, respectively, and the role of anammox in summer was higher than that in winter. Further, canonical correspondence analysis (CCA) proved the temperature is one of the key environmental variables, and nitri- & denitri- rates were primary microbial factors for anammox. Results suggested although the abundance of anammox bacteria was not high, the widespread of anammox played an overlooked role of N removal, which supplemented N balance calculation in biological wastewater treatment process, and provided theoretical support for the realization of anammox in the field of low-NH4+-N sewage treatment.
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Received: 21 January 2016
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