溶解氧对短程硝化稳定性及功能菌群的影响

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Abstract

The stability of partial nitrification at different dissolved oxygen (DO) concentration was studied in SBR at room temperature. Under the low DO concentration of 0.5mg/L, the partial nitrification kept stable for 75cycles with the nitrite accumulation rate (NAR) higher than 80%. Until 105cycles, the NAR gradually decreased and the partial nitrification transformed to the complete nitrification. However, using the same sludge, the partial nitrification kept long-term stable of 180cycles under the DO concentration of 2.5mg/L. Furthermore, NAR was higher than 90%. These results indicated under high DO concentration, the stable operation of partial nitrification can be maintained for a long period of time. The oxygen half saturation constant of AOB and NOB, and qPCR tests indicated that low DO might be benefit to the growth of Nitrospira that resulted in the unstable of the partial nitrification. Moreover, under high DO concentration, AOB had higher affinity for oxygen. Therefore, high oxygen concentration could maintain long-term stability of partial nitrification.

Key words： dissolved oxygen partial nitrification ammonia-oxidizing bacteria nitrite-oxidizing bacteria half-saturation constants of oxygen

Received: 23 January 2018

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	Articles by authors
	YANG Qing
	YANG Yu-bing
	YANG Zhong-qi
	HUANG Si-ting
	ZHOU Xue-yang
	LIU Xiu-hong

Cite this article:

YANG Qing,YANG Yu-bing,YANG Zhong-qi等. Effect of dissolved oxygen on the stability and functional microbial communities of the partial nitrification[J]. CHINA ENVIRONMENTAL SCIENCECE, 2018, 38(9): 3328-3334.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2018/V38/I9/3328

[1]	张可方,凌忠勇,荣宏伟,等.SBBR中DO对亚硝酸型同步硝化反硝化的影响[J]. 广州大学学报(自然科学版), 2008,7(6):54-58.
[2]	张立秋,韦朝海,张可方,等.常温下SBBR反应器中亚硝酸型同步硝化反硝化的实现[J]. 环境工程, 2009,27(1):40-43.
[3]	Park H D, Nogura D R. Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge[J]. Water Research, 2004.38(14):3275-3286.
[4]	孙洪伟,尤永军,赵华南,等.游离氨对硝化菌活性的抑制及可逆性影响[J]. 中国环境科学, 2015,35(1):95-100.
[5]	Siripong S, Rittmann B E. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants[J]. Water Research, 2007,41(5):1110−1120.
[6]	Hanaki K, Wantawin C, Ohagki S. Nitrification at low-levels of dissolved oxygen with and without organic loading in a suspended-growth reactor[J]. Water Research, 1990,24(3):297-302.
[7]	Blackburne R, Yuan Z. Partial nitrification to nitrite using low dissolved oxygen concentration as the main selection:factor[J]. Biodegradation, 2008,19,303-312.
[8]	张功良,李冬,张肖静,等.低温低氨氮SBR短程硝化稳定性试验研究[J]. 中国环境科学, 2014,34(3):610-616.
[9]	Zeng W, Li L, Yang Y, et.al. Nitritation and denitritation of domestic wastewater using a continuous anaerobic-anoxic-aerobic (A2O) process at ambient temperatures[J]. Bioresource Technology, 2010, 101(21):8074-8082.
[10]	Liu G, Wang J. Long-term low DO enriches and shifts nitrifier community in activated sludge[J]. Environment. Science. Technology, 2013,47(10):5109-5117.
[11]	Yang Q, Peng Y Z, Liu X H, et al. Nitrogen Removal via Nitrite form Municipal Wastewater at Low Temperatures using real-time Control to Optimize Nitrifying Communities[J]. Environment Science Technology, 2007,41(23):8159-8164.
[12]	复盛,国家环境保护总局,水和废水监测分析方法编委会,水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2002:38-47.
[13]	Nittami T, Ootake H, Imai Y, et al. Partial nitrification in a continuous pre-denitrification submerged membrane[J]. Biochemical Engineering Journal, 2011,55(2):101-107.
[14]	Regmi P. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research, 2014,57(2):162-171.
[15]	Ruiz G, Jeison D, Rubilar O. Nitrification-denitrification via nitrite accumulation for nitrogen removal from wastewaters[J]. Bioresource, 2016,8(2):113-119.
[16]	Hee D P, Daniel R N. Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge[J]. Water Research, 2004,38(5):3275-3286.
[17]	Daverey A, Su S H, Huang Y T. Partial nitrification and anammox process:A method for high strength optoelectronic industrial wastewater treatmen[J]. Water Research, 2013,47(2):2929−2937.
[18]	Blackburne R, Vadivelu V M, Yuan Z. Kinetic characterisation of an enriched Nitrospira culture with comparison to Nitrobacter[J]. Water Research, 2007,41(14):3033-3042.
[19]	Downing L S, Nerenberg R. Effect of oxygen gradients on the activity and microbial community structure of a nitrifying membrane-aerated biofilm[J]. Biotechnology and Bioengineering, 2008,101(6):1193-1204.
[20]	吴军,张悦,徐婷,等.AOB溶解氧亲和力低于NOB条件下序批反应器中NOB淘汰的实现机制[J]. 中国环境科学, 2016,36(12):3583-3590.
[21]	Stenstrom M K. The effect of dissolved oxygen concentration on nitrification[J]. Water Research, 1990,14(6):643-649.
[22]	Lucker S, Wagner M A. Nitrospirametagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria[J]. Water Research, 2010,107(30):13479-13484.
[23]	Guo J, Peng Y Z, Wang S Y. Long-term effect of dissolved oxygen on partial nitrification performance and microbial community structure[J]. Water Research, 2009,100(2):2796-2802.
[24]	Kim Y M, Cho H U. influence of operational parameters on nitrogen removal efficiency and microbial communities in a full-scale activated sludge process[J]. Water Research, 2011,17(1):5785-5795.
[25]	Bao P, Peng Y Z. Achieving partial nitrification by inhibiting the activity of Nitrospira-like bacteria under high-DO conditions in an intermittent aeration reactor[J]. Journal Of Environment science, 2016,8(2):113-119.