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| Effect of decreasing temperature on the performance and extracellular polymer substance of anaerobic ammonia oxidation sludge |
| SONG Cheng-kang, WANG Ya-yi, HAN Hai-cheng, CHEN Jie, WANG Xiao-dong, CHANG Qing-long |
| State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China |
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Abstract In this study, a long term dynamic change in anammox reaction in response to the gradient decreased and recovered temperature (33→25→20→15→10→22 ℃) was evaluated in a sequencing batch reactor (SBR) running over 360days; both the specific anammox activity (SAA) and extracellular polymers (EPS) contents were assessed. Results showed that anaerobic ammonia oxidation reaction in the SBR remained stable and efficient at 20~33℃; the total nitrogen removal load was 0.4gN/(L·d) and the SAA was over 0.32gN/(gVSS·d). The temperature of 10℃ was the turning point for the anammox bacteria metabolic activity. At 10℃, anammox treatment capacity reduced significantly, with the SAA being decreased by 91% when compared with that at 33℃. However, anammox activity could be recovered to 0.24gN/(gVSS·d) at 22℃. The apparent activation energy (Ea) values was 68.4kJ/mol at 10~33℃ and 152.9kJ/mol at 10~20℃. In the range of 15~33℃, the EPS content of the anammox biomass increased with decreasing temperature; however, after temperature decreased to 10℃, the EPS content dropped significantly, and anammox sludge broke up into smaller flocs, leading to the loss of biomass from the bioreactor.
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Received: 20 December 2015
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