|
|
Nitrogen removal characteristics and microbial community structure analysis of entrapped anaerobic ammonium oxidizing bacteria |
WANG Wei-qi, WANG Xiu-jie, LI Jun, WANG Si-yu |
The College of Architecture and Civil Engineering, Bejing University of Technology, Beijing 100124, China |
|
|
Abstract Using polyethylene glycol diacrylate as carrier materials, respectively with N, N, N, ‘N’-tetramethylethylenediamine (TEMED) as promoter and potassium persulfate (KPS) as initiator to immobilize anaerobic ammonium oxidizing bacteria. Orthogonal experiments were used to optimize immobilization conditions of anaerobic ammonium oxidation bacteria:10% PEGDA monomer, 0.25% KPS, 0.5% TEMED, the optimum operation condition was to control the polymerization temperature at 20℃, polymerization time was about 5min, The ratio of bacteria to gum was 1:1. The continuous flow experiment of immobilized pellets showed that after a short period of activity recovery, the nitrogen removal effect was continuously improved,and it had a certain impact resistance to the increase of hydraulic load. The scanning electron microscopy (SEM) showed that the immobilization materials have good biocompatibility and good mass transfer performance. High-throughput sequencing showed that after a period of time of stable operation, the microbial diversity in the pellets decreased slightly. And the Candidatus Kuenenia that belongs to anaerobic ammonium oxidizing bacteria which accounted for 6.58% of the total microbial system was up to 9.8%, the changes in microbial population showed that the performance of anaerobic ammonium oxidation could be improved greatly after immobilization.
|
Received: 05 March 2018
|
|
|
|
|
[1] |
Zhu W, Li J, Dong H, et al. Effect of influent substrate ratio on anammox granular sludge:performance and kinetics[J]. Biodegradation, 2017,28(5/6):437-452.
|
[2] |
Zhang L, Narita Y, Gao L, et al. Microbial competition among anammox bacteria in nitrite-limited bioreactors[J]. Water Research, 2017,125:249-258.
|
[3] |
Tang X, Guo Y, Jiang B, et al. Metagenomic approaches to understanding bacterial communication during the anammox reactor start-up[J]. Water Research, 2018,136:95-103.
|
[4] |
Jetten M S M, Wagner M, Fuerst J, et al. Microbiology and application of the anaerobic ammonium oxidation (‘anammox’) process[J]. Current Opinion in Biotechnology, 2001,12(3):283-288.
|
[5] |
祖波,张代钧,阎青.颗粒污泥厌氧氨氧化动力学特性及微量NO2的影响[J]. 环境科学, 2008,29(3):683-687.
|
[6] |
赵志宏,廖德祥,李小明,等.厌氧氨氧化微生物颗粒化及其脱氮性能的研究[J]. 环境科学, 2007,28(4):800-804.
|
[7] |
Qiao S, Tian T, Duan X, et al. Novel single-stage autotrophic nitrogen removal via co-immobilizing partial nitrifying and anammox biomass[J]. Chemical Engineering Journal, 2013,230:19-26.
|
[8] |
袁青,黄晓丽,高大文.不同填料UAFB-ANAMMOX反应器的脱氮效能[J]. 环境科学研究, 2014,27(3):301-308.
|
[9] |
申婷婷,李小明,岳秀,等.微生物固定化技术的研究与应用[J]. 广州化工, 2011,39(20):3-5.
|
[10] |
许晓毅,张婷婷,尤晓露,等.包埋固定化硝化污泥处理氨氮废水的过程特性[J]. 中国环境科学, 2016,36(10):2988-2996.
|
[11] |
Seo J K, Jung I H, Kim M R, et al. Nitrification performance of nitrifiers immobilized in PVA (polyvinyl alcohol) for a marine recirculating aquarium system[J]. Aquacultural Engineering, 2001, 24(3):181-194.
|
[12] |
Magrí A, Vanotti M B, Szögi A A. Anammox sludge immobilized in polyvinyl alcohol (PVA) cryogel carriers[J]. Bioresource Technology, 2012,114:231-240.
|
[13] |
钟成华,刘鹏,张文东,等.卡拉胶,膨润土改性PVA包埋小球[J]. 环境工程学报, 2013,7(8):2837-2843.
|
[14] |
唐崇俭,熊蕾,王云燕,等.高效厌氧氨氧化颗粒污泥的动力学特性[J]. 环境科学, 2013,34(9):3544-3551.
|
[15] |
Li Z, Zhang Z, Li J, et al. Comparative study of the nitrification characteristics of two different nitrifier immobilization methods[J]. Biodegradation, 2009,20(6):859-865.
|
[16] |
Isaka K, Kimura Y, Osaka T, et al. High-rate denitrification using polyethylene glycol gel carriers entrapping heterotrophic denitrifying bacteria[J]. Water Research, 2012,46(16):4941-4948.
|
[17] |
Isaka K, Date Y, Sumino T, et al. Ammonium removal performance of anaerobic ammonium-oxidizing bacteria immobilized in polyethylene glycol gel carrier[J]. Applied Microbiology and Biotechnology, 2007, 76(6):1457-1465.
|
[18] |
李泽兵,刘常敬,赵白航,等.多基质时厌氧氨氧化菌,异养反硝化污泥活性及抑制特征[J]. 中国环境科学, 2013,33(4):648-654.
|
[19] |
Strous M, Van Gerven E, Zheng P, et al. Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (anammox) process in different reactor configurations[J]. Water Research, 1997,31(8):1955-1962.
|
[20] |
陈光辉,李军,邓海亮,等.厌氧氨氧化污泥包埋固定化及其脱氮效能[J]. 北京工业大学学报, 2015,41(4):612-620.
|
[21] |
Bae W, Han D, Cui F, et al. Microbial evaluation for biodegradability of recalcitrant organic in textile wastewater using an immobilized-cell activated sludge process[J]. KSCE Journal of Civil Engineering, 2014,18(4):964-970.
|
[22] |
Du R, Cao S, Wang S, et al. Performance of partial denitrification (PD)-ANAMMOX process in simultaneously treating nitrate and low C/N domestic wastewater at low temperature[J]. Bioresource Technology, 2016,219:420-429.
|
[23] |
Cao S, Du R, Li B, et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater[J]. Applied Microbiology and Biotechnology, 2016,100(14):6457-6467.
|
[24] |
李滨,赵志瑞,马斌,等.克隆文库方法分析厌氧氨氧化反应器中细菌群落结构[J]. 环境科学与技术, 2012,1(12):159-164.
|
|
|
|