沉水植物轮叶黑藻附生细菌对双酚A的降解能力研究

Abstract
Figure/Table
References (49)
Related Citation (7)

Download: PDF (720 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Epiphytic bacteria of submerged macrophytes may have the capability in biodegradation and/or biotransformation of bisphenol A (BPA) in water column, therefore affect the fate of such environmental pollutant. In this research, Hydrilla verticillata (L. f.) Royle was selected and their attached BPA degrading epiphytic bacteria attached were isolated. Among the 22bacteria strains, the BPA removal rates were from 11.46% to 25.06% with the inoculum density at 1×10^-8cell/mL and culture at 37℃ for 72h. The most effective bacteria strains, B12, B14and B23 were identified as Lysinibacillus sp., Brevibacterium sp. and Ochrobactrum sp., respectively, according to the results of 16S rDNA sequencing and morphological, physiological and biochemical tests. But aseptic seedlings of H. verticillata significantly decreased their BPA removal rates after the addition with B12, B14and B23 (P<0.05). Natural seedlings of such species surprisingly increased about 5% in BPA removal after partially removing their epiphyte with physical methods. All the results indicated that epiphytic bacteria of submerged plant can remove BPA, although their contributions (about 23%) are less than the host plants in the submerged macrophytes-epiphyte associations.

Key words： epiphytic bacteria BPA removal submerged macrophytes

Received: 30 January 2016

:	X171.5
	X172

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHANG Guo-sen
	WANG Yu
	ZHUANG Xiao-jin
	YANG Shao
	JIANG Jin-hui

Cite this article:

ZHANG Guo-sen,WANG Yu,ZHUANG Xiao-jin等. Degradation of bisphenol A by the epiphytic bacteria of submerged macrophytes Hydrilla verticillata (L.f.) Royle[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(10): 3081-3088.

URL:

http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2016/V36/I10/3081

[1]	Michalowicz J. Bisphenol A - Sources, toxicity and biotransformation [J]. Environmental Toxicology and Pharmacology, 2014,37(2):738-758.
[2]	Chen J F, Xiao Y Y, Gai Z X, et al. Reproductive toxicity of low level bisphenol A exposures in a two-generation zebrafish assay: Evidence of male-specific effects [J]. Aquatic Toxicology (Amsterdam, Netherlands), 2015,169:204–214.
[3]	Alexander H C, Dill D C, Smith L W, et al. Bisphenol A: acute aquatic toxicity [J]. Environmental Toxicology and Chemistry, 1988,7:19-26.
[4]	Zhang B, Wu Z, Cheng S, et al. Primary study on photodegradation of bisphenol A by Elodea nuttallii [J]. Wuhan University Journal of Natural Sciences, 2007,12:1181-1124.
[5]	严贵芬.几种水生植物对水环境浓度BPA去除效果的研究 [D]. 华中师范大学, 2011.
[6]	邢维芹,骆永明,李立平,等.持久性有机污染物的根际修复及其研究方法 [J]. 土壤, 2004,36(3):258-263.
[7]	Chi J, Gao J. Effects of Potamogeton crispus L.–bacteria interactions on the removal of phthalate acid esters from surface water [J]. Chemosphere, 2015,119:59-64.
[8]	Mcguinness M, Dowling D. Plant-Associated Bacterial Degradation of Toxic Organic Compounds in Soil [J]. International Journal of Environmental Research & Public Health, 2009,6(8):2226-2247.
[9]	Weyens N, Lelie D V D, Taghavi S, et al. Phytoremediation: plant–endophyte partnerships take the challenge [J]. Current Opinion in Biotechnology, 2009,20(2):248-254.
[10]	蒋金辉,周长芳,安树青,等.工具种轮叶黑藻的组织培养与快速繁殖 [J]. 湖泊科学, 2008,20(2):200-215.
[11]	Zhang B G, Bai Z H, Hoefel D, et al. Assessing the impact of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere [J]. FEMS Microbiology Letters, 2008,284(1):102-108.
[12]	Sakai K, Yamanaka H, Moriyoshi K, et al. Biodegradation of bisphenol A and related compounds by Sphingomonas sp. strain BP-7isolated from seawater [J]. Bioscience Biotechnology & Biochemistry, 2007,71(1):51-7.
[13]	Toyama T, Kainuma Y, Kikuchi S, et al. Biodegradation of bisphenol A and 4-alkylphenols by Novosphingobium sp. strain TYA-1and its potential for treatment of polluted water [J]. Water Science & Technology A Journal of the International Association on Water Pollution Research, 2012,66(10):2202-8.
[14]	刘智,洪青,张晓舟,等.甲基对硫磷降解菌DLL—E4降解对-硝基苯酚特性 [J]. 中国环境科学, 2003,23(4):435-439.
[15]	Kang J H, Kondo F. Bisphenol A degradation in seawater is different from that in river water [J]. Chemosphere, 2005,60(9): 1288-1292.
[16]	Kuiper I, Lagendijk E L, Bloemberg G V, et al. Rhizoremediation: a beneficial plant-microbe interaction [J]. Molecular Plant- Microbe Interactions, 2004,17(17):6-15.
[17]	孙瑞珠,马玉龙,张娟,等.泰乐菌素降解菌的筛选及其降解动力学研究 [J]. 中国环境科学, 2013,33(4):722-727.
[18]	郑师章,乐毅全,吴辉,等.凤眼莲及其根际微生物共同代谢和协同降酚机理的研究 [J]. 应用生态学报, 1994,5(4):403-408.
[19]	Scheublin T R, Leveau J H J. Isolation of Arthrobacter species from the phyllosphere and demonstration of their epiphytic fitness [J]. Microbiologyopen, 2013,2(1):205-213.
[20]	Toyama T, Murashita M, Kobayashi K, et al. Acceleration of nonylphenol and 4-tert-octylphenol degradation in sediment by Phragmites australis and associated rhizosphere bacteria [J]. Environmental Science & Technology, 2011,45(15):6524-6530.
[21]	邓欢欢,张甲耀,赵磊,等.外源降解菌对黄麻根区净化能力的生物强化作用 [J]. 应用与环境生物学报, 2005,11(4):426-430.
[22]	胡立芳,沈东升,贺永华.接种Penicillium sp.和根际分泌物对土壤甲磺隆的协同降解研究 [J]. 环境科学, 2007,28(1):199-203.
[23]	Zhang W W, Yin K, Chen L X. Bacteria-mediated bisphenol A degradation [J]. Applied Microbiology and Biotechnology, 2013, 97(13):5681-5689.
[24]	Kang J H, Ri N, Kondo F. Streptomyces, sp. strain isolated from river water has high bisphenol A degradability [J]. Letters in Applied Microbiology, 2004,39(39):178-180.
[25]	Matsumura Y, Hosokawa C, Sasakimori M, et al. Isolation and characterization of novel bisphenol-A--degrading bacteria from soils [J]. Biocontrol Science, 2009,14(4):161-169.
[26]	Telke A A, Kalyani D C, Jadhav U U, et al. Purification and characterization of an extracellular laccase from a Pseudomonas sp. LBC1and its application for the removal of bisphenol A [J]. Journal of Molecular Catalysis B Enzymatic, 2009,61(3):252- 260.
[27]	Zhang C, Zeng G M, Yuan L, et al. Aerobic degradation of bisphenol A by Achromobacter xylosoxidans strain B-16isolated from compost leachate of municipal solid waste [J]. Chemosphere, 2007,68(1):181-190.
[28]	蒋俊,李秀艳,赵雅萍.2株分别降解壬基酚和双酚A细菌的分离,鉴定和降解特性 [J]. 环境科学研究, 2010,23(9):1196-1123.
[29]	邓伟光,谌建宇,李小明,等.壬基酚和双酚A降解菌株的分离,鉴定和特性研究 [J]. 环境科学学报, 2013,33(3):700-707.
[30]	Singh B, Kaur J, Singh K. Transformation of malathion by Lysinibacillus sp. isolated from soil [J]. Biotechnology Letters, 2012,34(5):863-7.
[31]	Babiak P, Kyslíková E, Štěpánek V, et al. Whole-cell oxidation of omeprazole sulfide to enantiopure esomeprazole with Lysinibacillus sp. B71 [J]. Bioresource Technology, 2011,102(17): 7621-6.
[32]	苏萌,陶然,杨扬,等.偶氮染料脱色菌Lysinibacillus sp.FS1的脱色性能 [J]. 环境工程学报, 2015,9(10):4664-4672.
[33]	曾锋,傅家谟,盛国英,等.不同菌源的微生物对邻苯二甲酸二异丁酯(DIBP)生物降解性的比较 [J]. 中国环境科学, 1999,19(4):322-324.
[34]	Strubel V, Engesser K H, Fischer P, et al. 3-(2-hydroxyphenyl) catechol as substrate for proximal meta ring cleavage in dibenzofuran degradation by Brevibacterium sp. strain DPO 1361 [J]. Journal of Bacteriology, 1991,173(6):1932-1937.
[35]	周建娇,邹芳,杨汉,等.乙草胺降解菌筛选及其初步鉴定 [J]. 中国土壤与肥料, 2013,(6):93-96.
[36]	Isabelle M, Villemur R, Juteau P, et al. Isolation of estrogen-degrading bacteria from an activated sludge bioreactor treating swine waste, including a strain that converts estrone to β-estradiol. [J]. Canadian Journal of Microbiology, 2011,57(7): 559-568.
[37]	Arulazhagan P, Vasudevan N. Biodegradation of polycyclic aromatic hydrocarbons by a halotolerant bacterial strain Ochrobactrum sp. VA1 [J]. Marine Pollution Bulletin, 2011, 62(2):388-94.
[38]	Qiu X H, Zhong Q Z, Li M, et al. Biodegradation of p- nitrophenol by methyl parathion-degrading Ochrobactrum sp. B2 [J]. International Biodeterioration & Biodegradation, 2007,59(4): 297–301.
[39]	Kang J H, Kondo f. Effects of bacterial counts and temperature on the biodegradation of bisphenol A in river water [J]. Chemosphere, 2002,49(5): 493-498.
[40]	王乐乐,张国森,耿超,等.狐尾藻降解双酚A内生菌的分离鉴定及降解特性 [J]. 环境科学与技术, 2015,38(12):16-21.
[41]	袁理,曾光明,张长,等.双酚A降解菌的分离鉴定及其降解特性 [J]. 环境科学, 2006,27(10):2095-2099.
[42]	房芳,张兰英,杨雪梅,等.双酚A高效降解菌的筛选与降解特性 [J]. 吉林大学学报(理学版), 2005,(6):873-876.
[43]	Saiyood S, Vangnai A S, Thiravetyan P. Bisphenol A removal by the Dracaena plant and the role of plant-associating bacteria [J]. Journal of Hazardous Materials, 2010,178(1-3):777-785.
[44]	李明堂,徐镜波,卢振兰,等.两株细菌对邻氯硝基苯的协同降解 [J]. 环境科学学报, 2010,30(6):1138-1143.
[45]	王佳楠,石妍云,郑力燕,等.石油降解菌的分离鉴定及4株芽胞杆菌种间效应 [J]. 环境科学, 2015,(6):2245-2251.
[46]	刘娟,凌婉婷,盛月慧,等.根表功能细菌生物膜及其在土壤有机污染控制与修复中的潜在应用价值 [J]. 农业环境科学学报, 2013,32(11):2112-2117.
[47]	Yamaga F, Washio K, Morikawa M. Sustainable biodegradation of phenol by Acinetobacter calcoaceticus P23 isolated from the rhizosphere of duckweed Lemna aoukikusa[J]. Environmental Science and Technology, 2010,44(16):6470-6474.
[48]	Molina M A, Ramos J L, Espinosa U M. Plant-associated biofilms [J]. Reviews in Environmental Science and Bio/ Technology, 2003,(2):99-108.
[49]	Baines S B, Pace M L. The production of dissolved organic matter by phytoplankton and its importance to bacteria: Patterns across marine and freshwater systems [J]. Limnology & Oceanography, 1991,36(6):1078-1090.