低温耐盐芘降解菌的筛选鉴定及降解特性研究

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Abstract The community H and single-strain DYC-1 was isolated by timing quantitative culture and domestication method of adding the concentration of PAHs gradatim from Tianjin coastal wetland petroleum contaminated soil under the low temperature condition. The community H and single-strain DYC-1used pyrene as solo carbon source for growth. The bacteria were identified as Rhodococcus sp. based on the BLAST sequence analysis of 16s rDNA and its morphological and physio-biochemcial characteristics. Analysis results showed that the degrading ability of single-strain DYC-1 was similar to community H under the low temperature condition. The degradation rate of them was above 35% at high concentrations of pyrene. The single-strain DYC-1 had more salt-resisting ability and wide substrate degradation ability. The optimal degradation conditions of single-strain DYC-1 was 2% salinity, pH8, 10℃, 110r/min rotate speed and 5% capacity of bacteria. The degradation rate of pyrene was high with 20mg/L initial concentration in the optimal degradation conditions.

Key words： cold-resistant haloduric pyrene degradation bioremediation

Received: 10 April 2016

PACS:

X172

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	DIAO Shuo
	WANG Hong-qi
	XU Jie
	ZHAO Yi-cun

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DIAO Shuo,WANG Hong-qi,XU Jie等. Isolation, identification and analysis of degradation ability of a cold-resistant haloduric pyrene-degrading strains[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(2): 677-685.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I2/677

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