海洋异养硝化-好氧反硝化菌y6同步脱氮除碳特性

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Abstract A heterotrophic nitrification-aerobic denitrification strain, named y6, was isolated from sediment of Jiaozhou Bay. It was identified as Klebsiella sp. based on the morphological, physiological and analysis of 16S rRNA gene sequence. Under different environmental conditions, measured strain y6's growth situation and denitrification ability, so as to analyze its characteristics of simultaneous removal of nitrogen and carbon. The results showed that the optimal carbon source was sodium citrate; the optimal pH was 7.0; the optimal C/N was 17. In the reaction system of NH₄Cl, KNO₃ and NaNO₂ for the only nitrogen source, Strain y6had better denitrification effect. The removal efficiencies were 99.67%, 100% and 99.20%. Strain y6could efficiently remove organic matter simultaneously in the process of denitrification, with COD removal rate of 82.17%, 95.75% and 97.83%, respectively. Almost no nitrate nitrogen and nitrite nitrogen accumulation in the y6 heterotrophic nitrification process. In the different mixing ratio of reaction system, the first were aerobic denitrification of nitrate nitrogen, Subsequent were ammonia nitrogen, nitrite nitrogen and COD removal. Ammonia nitrogen removal rate was only slightly lower in the presence of the nitrate nitrogen. The nitrite nitrogen affected y6heterotrophic nitrification process. The heterotrophic nitrification of y6had no effect on aerobic denitrification.

Key words： heterotrophic nitrification-aerobic denitrification marine bacterium Klebsiella sp. y6 Simultaneous removal of nitrogen and carbon

Received: 23 June 2016

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X172

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	Articles by authors
	WANG Xiao-jing
	YU De-shuang
	LI Jin
	DU Song-dong
	ZHOU Tong
	WU Guo-dong

Cite this article:

WANG Xiao-jing,YU De-shuang,LI Jin等. Separation identification and the characteristics research of simultaneous removal of nitrogen and carbon about Marine heterotrophic nitrification and aerobic denitrification strain y6[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(2): 686-695.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I2/686

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