|
|
|
| Characteristics of microbial community structure in the stable operation of the partial cut nitrification system with seasonal temperature |
| ZHAO Xin-yan1, BIAN Wei1, HOU Ai-yue2, KAN Rui-zhe1, ZHAO Qing1, LI Jun1 |
1. College of Architecture Engineering, Beijing University of Technology, Beijing 100124, China;
2. Beijing Guohuan Tsinghua Environment Engineering Design and Research Institute, Beijing 100084, China |
|
|
|
|
Abstract In order to investigate the effect of seasonal temperature on microbial community structure of nitrification biofilm system, the nitrification biofilm system in MBBR reactor under the temperature of 28.3,23.3,21.6,9.6 and -0.2℃ were studied by using denatures gradient gel electrophoresis polymerase chain reaction(PCR-DGGE) technique. The activity of bacteria affected by loss of the water temperature, but did not affect stability of nitrification. During the change of water temperature(21.6~0.2℃), the total bacteria and AOB stayed stable; and the waste-water treatment of Bacillus, Flavobacterium and Nitrosomonas also stayed stable. The dominant bacteria in biofilm mainly was Proteobacteria and Bacteroidetes under the average temperature of 28.3~-0.2℃, and the AOB was Nitrosomonas that was the part of β-Proteobacteria. The partial nitrification under low temperature was achieved by controlling HRT merely, and it made great sense for the application of shortcut nitrification process.
|
|
Received: 29 August 2016
|
|
|
|
|
|
| [1] |
高春娣,王惟肖,李 浩,等.SBR法交替缺氧好氧模式下短程硝化效率的优化[J]. 中国环境科学, 2015,35(2):403-409.
|
| [2] |
孙洪伟,杨 庆,董国日,等.游离氨抑制协同过程控制实现渗滤液短程硝化[J]. 中国科学:化学, 2010,40(8):1156-1162.
|
| [3] |
张功良,李 冬,张肖静,等.低温低氨氮SBR短程硝化稳定性试验研究[J]. 中国环境科学, 2014,34(3):610-616.
|
| [4] |
Rivasim, Arvin F. Side stream biofilm for im-proved biofouling control in cooling water system[J]. Water Science & Technology, 2001,41:445-451.
|
| [5] |
Paul L B, Tong Y.A microelectrode study of redox potential chain biofilms[J]. Water Science & Technology, 1999,39(7):179-185.
|
| [6] |
Massoldeya A A, Whallon J, Hickey R F, et al. Channel structures in aerobic biofilms of fixed -film reactors treating contaminated groundwater[J]. Applied and Environmental Microbiology, 1995,61(3):769-777.
|
| [7] |
Chrysi S L, Bruce E R. Modeling the development of biofilm density including active bacteria, inert bio-mass, and extracellular polymeric substances[J]. Water Research, 2004,38:3349-3361.
|
| [8] |
侯爱月,李 军,卞 伟,等.不同好氧颗粒污泥中微生物群落结构特点[J]. 中国环境科学, 2016,36(2):428-436.
|
| [9] |
Kowalchuk G A, Bodelier P L E, Heilig G H J, et al. Community analysis of ammonium-oxidizing bacteria,in relation to oxygen availability in soils and root-oxygenated sediments, using PCR, DGGE and oligonucleotide probe hybridization[J]. FEMS Microbiology Ecology, 1998,24:339-350.
|
| [10] |
Hellinga C, Schellen A A, Mulder J W, et al. The sharon process:An innovative method for nitrogen removal from ammonium-rich wastewater[J]. Water Science & Technology, 1998,37(9):135-142.
|
| [11] |
杨 庆,彭永臻,王淑莹,等.SBR法低温短程硝化实现与稳定的中试研究[J]. 化工学报, 2007,58(11):2901-2905.
|
| [12] |
崔 迪.寒区污水生化处理系统微生物群落结构与功能解析[D]. 哈尔滨::哈尔滨工业大学, 2014.
|
| [13] |
Hoang V, Delatolla R, Abujamel R, et al. Nitrifying moving bed biofilm reactor (MBBR) biofilm and biomass response to long term exposure to 1℃[J]. Water Research, 2014,49:215-224.
|
| [14] |
Wagner M, Loy A. Bacterial community composition and function in sewage treatment systems[J]. Current Opinion in Biotechnology, 2002,13(3):218-227.
|
| [15] |
郝桂玉,徐亚同,黄民生,等.芽孢杆菌脱氮作用的研究[J]. 环境科学与技术, 2004,27(1):20-21,37.
|
| [16] |
韩晓云.低温生物膜及其微生物特性研究[D]. 哈尔滨:哈尔滨工业大学, 2006.
|
| [17] |
Flores III A, Nisola G M, Cho E, et al. Bioaugmented sulfur- oxidizing denitrification system with Alcaligenes defragrans B21forhigh nitrate containing wastewater treatment[J]. Bioprocess and Biosystems Engineering, 2007,30(3):197-205.
|
| [18] |
Limpiyakorn T, Kurisu F, Sakamoto Y, et al. Effects of ammonium and nitrite oncommunities and populations of ammonia-oxidizing bacteria in laboratory-scale continuous-flow reactors[J]. Fems Microbiology Ecology, 2007,60(3):501-512.
|
| [19] |
Van Dyke S, Jones S, Ong S K. Cold weather nitrogen removal deficiencies of aerated lagoons[J]. Environmental Technology, 2003,24(6):767-777.
|
| [20] |
Buswell A M, Shiota T, Lawrence N, et al. Laboratory studies on the kinetics of the growth of Nitrosomonas with relation to the nitrification phase of the B.O.D test[J]. Applied Microbiology, 1954,2(1):21-25.
|
| [21] |
Mc Cartney D M, Oleszkiewicz J A. Carbon and nutrient removal in a sequencing batch reactor at low temperatures[J]. Environmental Technology, 1990,11(2):99-112.
|
| [22] |
Painter H A,Loveless J E. Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge process[J]. Water Research, 1983,17(3):237-248.
|
| [23] |
Delatolla R, Tufenkji N, Comeau Y, et al. Effects of long exposure to low temperatures on nitrifying biofilm and biomass in wastewater treatment[J]. Water Environment Research, 2012, 84(4):328-338.
|
| [24] |
张 岩,孙凤侠,谢杭冀,等.四区一体反应器冬季启动脱氮特性及硝化菌群结构分析[J]. 环境科学, 2015,36(9):3339-3344.
|
| [25] |
Jones R D, Mo ri ta R Y, Koops H P, et al. A new marine am monium-oxidizing bacterium Nitrosomonas cryotolerans sp. nov.[J]. Can. J. Microbiol., 1988,34(10):1122-1128.
|
| [26] |
Purkhold U, Pommerening-röser A, Juretschko S. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amo A sequence analysis:implications for molecular diversity surveys[J]. Applied Environmental Microbiology, 2000,66(12):5368-5382.
|
| [27] |
Wells G F, Park H D, Yeung C H, et al. Ammonia-oxidizing communities in a highly aerated full-scaleactivated sludge bioreactor:betaproteobacterial dynamics and low relative abundance of Crenarchaea[J]. Environmental Microbiology, 2009,11(9):2310-2328.
|
| [28] |
Zhang T, Ye L, Tong A, et al. Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors[J]. Applied Microbiology and Biotechnology, 2011,91(4):1215-7660.
|
| [29] |
Yu T, He P J, Lü F, et al. Mediating N2O emissions from municipal solid waste landfills:Impacts of landfill operating conditions on community structure of ammonia-oxidizing bacteriain cover soils[J]. Ecological Engineering, 2009,35(5):882-889.
|
|
|
|
