核呼吸因子1在心肌细胞损伤中的保护作用

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Abstract

We constructed a NRF1-pcDNA3.1 plasmid and transfected it in H9C2 cells to explore the effects of NRF1 in sodium bisulfite-induced mitochondrial damage in cardiac muscles of rats. H9C2 cells were treated with 100mmol/L NaHSO₃ for 24h after NRF1-pcDNA3.1 or pcDNA3.1 transfection. Protein levels of NRF1 and TFAM were measured by Western blotting. The amount of ATP was detected by the chemiluminescence method. It showed that NaHSO₃ treatment for 24h resulted in significant decreases of NRF1 and TFAM protein expression and ATP amount. The protein levels of NRF1 and TFAM were significantly elevated after NRF1-pcDNA3.1 plasmid transfection, companied by a significant increase of ATP amounts. NRF1and TFAM protein expressions and ATP amount significantly increased after NaHSO₃ exposure for 24h in NRF1 over-expression group, compared with the pcDNA3.1-transfection group. It implied that over-expression of NRF1 might regulate TFAM to increase intracellular ATP production. In addition, over-expression of NRF1 alleviated the dysfunction of mitochondrial ATP caused by NaHSO₃ exposure. NRF1 plays an important role in the regulation of NaHSO₃-induced mitochondrial damage.

Key words： NRF1 TFAM NaHSO₃ over-expression plasmid

Received: 21 July 2016

PACS:

X503

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	Articles by authors
	QIN Guo-hua
	WANG Rui-xia
	XU Zhi-fang
	SANG Nan

Cite this article:

QIN Guo-hua,WANG Rui-xia,XU Zhi-fang等. The protect role of nuclear respiratory factor 1 in mitochondrial damage in cardiac muscles[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(4): 1556-1561.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I4/1556

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