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| Study on the removal of Cd(II) by Pseudomonas nitroreducens: Biosorption characteristics and mechanism |
| YU Yong-quan1,2, HUANG Wei-wei3, DONG Jian-jiang4, ZHU Qi-fa3, LU Dian-nan2, LIU Yong-min1 |
1. College of Petroleum and Chemical Engineering, Liaoning Shihua University, Fushun 113001, China;
2. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
3. Anhui Wannan Tobacco Liability Company, Xuancheng 242000, China;
4. China Tobacco Corporation, Hefei 230022, China |
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Abstract The adsorption characteristics and adsorption mechanism of cadmium removal by Pseudomonas nitroreducens were investigated systematically. The results showed that the strain could tolerate cadmium up to 200mg/L. Further increase the concentration of cadmium led to a significantly inhibition of the growth of Pseudomonas nitroreducens. In a liquid culture of Pseudomonas nitroreducens, where a liquid LB medium containing 20mg/L, 50mg/L or 100mg/L Cd2+ was used, the removal rate of cadmium reached 94.3% 91.0% and 86.0%, respectively after 120h of cultivation. The impact of pH, temperature, salt concentration and various other heavy metal ions on the removal efficiency of cadmium were also explored. The results showed that Pseudomonas nitroreducens effectively adsorbed cadmium with in the pH range from 4 to 8. The osmosis stress test showed that Pseudomonas nitroreducens could tolerate a NaCl solution of 1mol/L. Under this condition, the strain still adsorbed a variety of heavy metal ions except lead ions. X-ray photoelectron spectroscopy analysis showed that the binding energy of cadmium changed after adsorption. Scanning electron microscopy (SEM) showed that Pseudomonas nitroreducens deformed and has white particles attached on the surface after cadmium treatment, indicating that biomineralization is one of its ways to adsorb heavy metals.
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Received: 19 October 2016
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