二级出水中环丙沙星耐药菌光辐照灭活研究

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Abstract

A ciprofloxacin-resistant bacterium strain was isolated from secondary treated effluent,and effects of light irradiation for disinfection of ciprofloxacin-resistant bacterium strain were investigated.The ciprofloxacin-resistant bacterium strain presented resistance to penicillin,ampilicillin,sulfamethoxazole,tetracyline and rifampicin.In the presence of ciprofloxacin,the maximum specific growth rate of the strain decreased from 0.63h^-1 to 0.51h^-1.The inactivation ratio of ciprofloxacin-resistant bacterium strain raised with increasing of light intensity and irradiation time,and the inactivated reaction followed either the zero order or first order kinetics.By irradiation of visible light (100/300/500W mercury lamp and 1000W xenon lamp (>400nm)) for 60min,the inactivation ratio of ciprofloxacin-resistant bacterium strain reached 0.25~0.39log.The inactivated reaction by 100/300W mercury lamp and 1000W xenon lamp (>400nm) irradiation fited in with zero order kinetics,and the reaction rate constant was 10196.43~11345.24CFU/(mL·min).The inactivated reaction by 500W mercury lamp (>400nm) followed first order kinetics,and the reaction rate constant was 0.01min^-1.By irradiation of visible light with UVA (100/300/500W mercury lamp and 1000W xenon lamp (>300nm)) for 60min,the inactivation ratio of ciprofloxacin-resistant bacteria reached 0.30~5.63log.The inactivated reaction by 100W mercury lamp (>300nm) followed first order kinetics,and the reaction rate constant was 0.01min^-1.The inactivated reaction by 300W mercury lamp (>300nm) irradiation fited in with zero order kinetics,and the reaction rate constant was 2572.02CFU/(mL·min).Both of photo reactivation and dark repair took place when ciprofloxacin-resistant bacteria were not completely inactivated.The reactivation ratio reached-3.9~123.4% after photo reactivation of 48h and dark repair.During light irradiation,the ciprofloxacin resistance of the strain was only affected by 1000W visible light with UVA irradiation.By irradiation for 60min,its inhibition diameter decreased from 11.0mm to 8.0mm.

Key words： ciprofloxacin-resistant bacteria visible light UVA inactivation ratio ciprofloxacin resistance

Received: 28 November 2016

PACS:	X703.1
	X172

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	Articles by authors
	SHI Na
	SUN Ying-xue
	Qi Fei
	Hu Chun-fang

Cite this article:

SHI Na,SUN Ying-xue,Qi Fei等. Inactivate ciprofloxacin-resistant bacteria in secondary treated effluent by light irradiation[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(7): 2599-2606.

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http://www.zghjkx.com.cn/EN/ OR http://www.zghjkx.com.cn/EN/Y2017/V37/I7/2599

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