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| Discussion of the measurement method of ambient bioaerosols based on fluorescent signal |
| REN Yi1, HUANG Xiao-feng1, WANG Zhi-bin2, SUN Tian-le3, SUN Jie3, ZHANG Ming-di3, HE Ling-yan1 |
1. Key Laboratory for Urban Habitat Environmental Science and Technology, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China;
2. Multiphase Chemistry Department, Max-Planck Institute for Chemistry, Mainz 55128, Germany;
3. Shenzhen Environmental Monitoring Center, Shenzhen 518049, China |
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Abstract A Wideband Integrated Bioaerosol Spectrometer (WIBS) was operated continuously from August 1to August 31 in 2015 at an urban sampling site in Shenzhen, China. The average number concentrations of the three fluorescent channels, i.e., FL1, FL2 and FL3, were 0.41, 0.61 and 0.24cm-3, respectively. The fluorescent aerosol particles (FAPs) was further divided into seven types according to their fluorescence thresholds. The mean number concentrations of the two dominant types, i.e., Type B and Type BC, were 0.214cm-3 and 0.202cm-3. Different diurnal variations of fluorescent particles was also observed, but they generally showed a low value in the daytime and a high value at night. Most of the number size distributions of FAPs showed a peak at 1.1μm except Type ABC, whose peak was at 2.2μm. In order to discuss the influence of non-biological fluorescence components on bioaerosol measurement, the correlation analysis and principal component analysis among black carbon, acetonitrile, m/z44, and different FAP types were applied. The results showed that most of the FAP types were interfered by different non-biological components, but Type C showed a weak correlation with all the pollution source markers. The mean number concentration of Type C was 0.016cm-3, which could be a more reliable value for the real bioaerosols. This study found that non-biological fluorescent components had a large influence on the measurement of bioaerosols based on the fluorescence detection in urban environment.
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Received: 26 December 2016
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