固相碳源脱氮同步去除壬基酚的试验研究

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Abstract

The simultaneous nitrification and denitrification bioreactors in this research were packed with a new type of solid phase carbon source NS, which was invented based on PHBV. The removal rate of nitrogen and nonylphenol (NP) in the reactor was investigated. Illumina MiSeq high-throughput sequencing and quantitative PCR (qCPR) were applied to analyze the microbial community structure and functional genes. The removal rates of nitrate and ammonia nitrogen were 96.18% and 82.54%, respectively. Average COD concentration was 27.48mg/L in effluent.The average removal efficiency of NP was 81.17%. The results of high-throughput sequencing illustrated that the previously reported denitrifiers including Dechloromonas, Rhodocyclaceae and Zoogloea were the dominant microbe genus on the surface of solid carbon when NP was not added. The estimated total number of bacteria species and α diversity decreased after NP was added in the influent. According to outcomes of qPCR, functional genes of nitrogen cycle had rich abundance in the bioreactor. Abundances of nirS gene and amoA gene were the highest and lowest, respectively. However, nirS abundance reduced when NP was added in the influent.

Key words： solid phase carbon source simultaneous nitrification and denitrification nonylphenol microbial community structure functional genes

Received: 26 December 2016

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X522

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	WANG Ting
	SUN Jia-ning
	WU Wei-zhong

Cite this article:

WANG Ting,SUN Jia-ning,WU Wei-zhong. Simultaneous removal of nitrogen and nonylphenol using solid phase carbon source[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(8): 2915-2923.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I8/2915

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