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| Physiological and bio-chemical effects of pure MC-LR and Microcystis aeruginosa crude extracts on Oryza sativa L.at vegetative stage |
| ZHANG Hui1,2, JIANG Jin-lin1, ZHANG Yu-feng2, SHAN Zheng-jun1 |
1. Key laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2. College of Environment, Nanjing Tech University, Nanjing 210009, China |
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Abstract In the present study, the changes of plant height, root length, plant fresh weight, amylase activity, AKP activity, GSH content and MDA content of rice (Oryza sativa L.) were investigated under the exposure to a series of concentrations of MC-LR (0.1μg/L, 1.0μg/L and 10.0μg/L) and Microcystis aeruginosa crude extracts (0.002lysate, 0.02lysate and 0.2lysate) for 21d. Results showed that the plant height, root length and amylase activities of rice were decreased under the exposure of pure MC-LR. Root length was a sensitive indicator in rice for its quick response to MC-LR exposure in 0.1μg/LMC-LR treatment group. However, Microcystis aeruginosa crude extracts with high concentration of MC-LR had positive effects on growth and development of rice except for root length. GSH content of rice was significantly induced in 0.1μg/L MC-LR group, but no significant changes of AKP activity was observed in all pure MC-LR treatment groups. In contrast, GSH content, MDA content and AKP activity could be significant inhibited in rice exposed to Microcystis aeruginosa crude extracts. These results suggested that the toxicity mechanism of MC-LR to rice might be different from Microcystis aeruginosa crude extracts and other toxic components existed in Microcystis aeruginosa crude extracts may exert great influence on rice.
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Received: 20 January 2017
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