硫自养微生物降解水中低浓度高氯酸盐的研究——反应器效能及微生物种群空间分布

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Abstract

Removal of perchlorate with low concentration[(468.74±6.80)μg/L] in water was investigated by an up-flow sulfur autotrophic reduction reactor. And bacterial community spatial distribution was analyzed by High-throughput sequencing method. The reactor could be operated at a hydraulic retention time (HRT) ranging in 4.00~0.75h with a remarkable removal efficiency greater than 97%. 1/2-order kinetics model fit the experimental data well; and 1/2K_1/2v was 39.59[μg^1/2/(L^1/2·h)]. When HRT shortened from 4.00h to 0.75h, the generated SO₄^2- decreased from 173.37 to 90.07mg/L. Sulfur (S) disproportionation was accompanied with perchlorate reduction; the proportion of SO₄^2- generated by S-disproportionation was in range of 90.75%~93.91%. Meanwhile, S-disproportionation was the main reason for excess consumption of alkalinity, thus leading to pH decreases in effluent. The sequencing results showed that the α-biodiversity was decreased along the height of reactor. The Proteobacteria and Chlorobi was observed as the major bacteria, and the Chlorobaculum was the dominant bacteria associated with S-disproportionation.

Key words： perchlorate sulfur autotrophic kinetics S-disproportionation High-throughput sequencing bacterial community

Received: 16 November 2016

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	LIU Yong-de
	WANG Yi-yi
	WAN Dong-jin
	XIAO Shu-hu

Cite this article:

LIU Yong-de,WANG Yi-yi,WAN Dong-jin等. Bio-reduction of perchlorate with low concentration in water by sulfur packed reactor and microbial community spacial distribution analysis[J]. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(8): 3142-3150.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2017/V37/I8/3142

[1]	Urbansky E T. Perchlorate chemistry:Implication for analysis and remediation[J]. Bioremediation Journal, 1998,2(2):81-95.
[2]	Charnley G. Perchlorate:Overview of risks and regulation[J]. Food and Chemical Toxicology, 2008,46:2307-2315.
[3]	Tieman M. Perchlorate contamination of drinking water:Regulatory issue and legislative actions[R]. CRS Repo~for Congress, Order Code RS21961, Updated April 4, 2007.
[4]	Logan B E. Assessing the outlook for perchlorate remediation[J]. Environmental Science & Technology, 2001,35(23):482-487.
[5]	Bardiya N, Bae J H. Dissimilatory perchlorate reduction:A review[J]. Microbiological Research, 2011,166:237-254.
[6]	Ju X, Sierra-Alvarez R, Field J A, et al. Microbial perchlorate reduction with elemental sulfur and other inorganic electron donors[J]. Chemosphere, 2008,71(1):114-122.
[7]	Ju, X, Field J A, Sierra-Alvarez R, et al. Chemolithotrophic perchlorate reduction linked to the oxidation of elemental sulfur[J]. Biotechnol. Bioeng., 2007,96(6):1073-1082.
[8]	谢杰,杨志泉,陈兵,等.硫自养菌降解还原高氯酸盐的优化控制研究[J]. 水处理技术, 2012,6(6):32-35.
[9]	任云,高孟春,王子超,等.硫自养高氯酸盐还原菌和反硝化菌的培养和驯化[J]. 应用与环境生物学报, 2014,20(3):414-419.
[10]	Sahu, A K, Conneely T, Nüsslein K R, et al. Biological Perchlorate Reduction in Packed Bed Reactors Using Elemental Sulfur[J]. Environmental Science & Technology, 2009,43:4466-4471.
[11]	万东锦,刘永德,樊荣,等.硫自养填充床生物反应器去除水中的高氯酸盐[J]. 环境工程学报, 2015,9(11):213-218.
[12]	王绍祥,杨洲祥,孙真,等.高通量测序技术在水环境微生物群落多样性中的应用[J]. 化学通报, 77(3):196-203.
[13]	Zhang T, Shao M F, Ye L, et al. 454pyrosequencing reveals bacterial diversity ofactivated sludge from 14sewage treatment plants[J]. ISME Journal, 2011,6:1137-1147.
[14]	Ontiveros-Valencia A, Tang Y, Zhao H P, et al. Pyrosequencing analysis yields comprehensive assessment of microbial communities in pilot-scale two-stage membrane biofilm reactors[J]. Environmental Science & Technology, 2014,48:7511-7518.
[15]	Boles A R, Conneely T, McKeever R, et al. Performance of a pilot-scale packed bed reactor for perchlorate reduction using a sulfur oxidizing bacterial consortium[J]. Biotechnogy and Bioengineering, 2012,109(3):637-646.
[16]	国家环保总局《水和废水分析监测方法》编委会.水和废水分析监测方法[M]. 4版.北京:中国环境科学出版社, 2009.
[17]	Wan D, Liu Y, Niu Z, et al. Perchlorate reduction by hydrogen autotrophic bacteria and microbial community analysis using high-throughput sequencing[J]. Biodegradation, 2016,27:47-57.
[18]	Konenig A, Liu LH. Kinetic model of autotrophic denitrification in sulfur packed-bed reactors[J]. Water Research, 2001,35(8):1969-1978.
[19]	Moon H S, Ahn K H, Lee S, Nam K, et al. Use of autotrophic sulfur-oxidizers to remove nitrate from bank filtrate in a permeable reactive barrier system[J]. Environmental Pollution, 2004,129(3):499-507.
[20]	Rodriguez J, Hiras J, Hanson T E. Sulfite oxidation in chlorobaculum tepidum[J]. Frontiers in Microbiology, 2011,2:112.
[21]	Zhang M, Zhang T, Shao M F, et al. Autotrophic denitrification in nitrate-induced marine sediment remediation and Sulfurimonas denitrificans-like bacteria[J]. Chemosphere, 2009,76(5):677-682.