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Study on ANAMMOX process with alanine as substrate |
XU Min, GAO Da-wen |
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150000, China |
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Abstract The short-term and long-term effects of alanine on ANAMMOX process were investigated by batch experiments. The ANAMMOX process was greatly affected both in short-term and long-term culture when alanine was the sole substrate, and there was no nitrogen removal in the system. Without electron acceptor NO2--N, ANAMMOX process could not occur in the system, although the alanine removal could reach more than 86%, NH4+-N was accumulated in the system. When NH4+-N and NO2--N were added to the system with alanine as a substrate, the activity of ANAMMOX bacteria was not affected so much in the short term (7h), and 2mmol/L and 10mmol/L alanine reached 78% and 99% removal efficiencies in 10 and 60h, respectively. During the long term experiments, the activity of ANAMMOX bacteria could be inhibited by alanine with a concentration of 2mmol/L. Combined with ANAMMOX and denitrification processes, the removal efficiency of TN reached 57%, and the removal efficiency of alanine was about 99%.
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Received: 11 March 2017
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