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| Denitrification performance of the bacterium Diaphorobacter polyhydroxybutyrativorans SL-205 |
| ZHANG Shu-song1,2, FAN Yue-ting1,2, SUN Xing-bin1, QIU Tian-lei2, GAO Min2, WANG Xu-ming2,3 |
1. College of Life Science, Northeast Forestry University, Harbin 150040, China;
2. Beijing Agro-Biotechnology Research Center, Beijng Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
3. Key Laboratory of Urban Agriculture(North), Ministry of Agriculture, Beijing 100097, China |
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Abstract In order to enhance nitrogen removal from nitrate-contaminated water, a novel bacterial strain, Diaphorobacter polyhydroxybutyrativorans SL-205T, was investigated to use different carbon sources for aNOxic denitrification. Additionally, the aerobic denitrification performance for strain SL-205T was studied using poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) as the sole carbon source. The results showed that the removal efficiencies of nitrate reached up to over 99% by strain SL-205T using sodium acetate, sodium succinate and PHBV as carbon sources under the aNOxic conditions. The optimum addition dosage of PHBV was 2.0g/L with 315mg/L of the initial nitrate nitrogen. Strain SL-205T had the capability to fully remove as much as 97.4% of nitrate nitrogen, and the average removal rate for nitrate nitrogen was 8.69mg/(L·h) after 36h under the aerobic conditions. Meanwhile, both nitrite and nitrous oxide were not accumulated. The results above-mentioned would lay an experimental foundation for nitrogen removal from wastewater by strain SL-205T.
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Received: 18 January 2017
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| [1] |
Zhang J B, Wu P X, Hao B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas strtzeri YZN-001[J]. Bioresource Technology, 2011,102:9866-9869.
|
| [2] |
信欣,姚力,鲁磊.耐高氨氮异养硝化-好氧反硝化菌TN-14的鉴定及其脱氮性能[J]. 环境科学, 2013,35(10):3926-3932.
|
| [3] |
全向春,岑艳,钱殷.2株好氧反硝化菌的筛选及其强化贫营养生物膜脱氮效果[J]. 环境科学, 2013,34(7):2862-2868.
|
| [4] |
刘道玉,吴伟.水产养殖水体污染及微生物修复的研究[J]. 现代农业科技, 2011,(17):253-256.
|
| [5] |
秦伯强,高光,朱广伟,等.湖泊富营养化及其生态系统响应[J]. 科学通报, 2013,58(10):855-864.
|
| [6] |
Rafidah H, Tengku N Z, Izzati I I. Nitrate Removal from Domestic Wastewater by Using Denitrification Limestone Filter[J]. Advanced Materials Research, 2014,3607(1051):578-582.
|
| [7] |
Huan H, Wang J S, Lai D S. Detection of denitrification on six soils in Jilin City of Northeast China[J]. Environment and Sustainability, 2014,154:189-196.
|
| [8] |
刘博,肖长来,梁秀娟,等.吉林市城区浅层地下水污染源识别及空间分布[J]. 中国环境科学, 2015,35(2):457-464.
|
| [9] |
王战蔚,张译丹,李秀颖,等.池塘中氨氮、亚硝酸盐的危害及控制措施[J]. 吉林水利, 2013,(3):39-48.
|
| [10] |
李梦露,蒋建国,张昊巍.餐厨垃圾水解酸化液作碳源的脱氮效果研究[J]. 中国环境科学, 2014,34(4):917-923.
|
| [11] |
Hajesh R P, Anshuman A K, Hemant J P. Identification and monitoring of nitrification and denitrification genes in Klebsiella pneumoniae EGD-HP19-C for its ability to perform heterotrophic nitrification and aerobic denitrification[J]. Functional & Integrative Genomics, 2015,(15):63-76.
|
| [12] |
Ge Q, Yue X P, Wang G Y. Simultaneous heterotrophic nitrification and aerobic denitrification at high initial phenol concentration by isolated bacterium Diaphorobacter sp. PD-7[J]. Chinese Journal of Chemical Engineering, 2015,(23):835-841.
|
| [13] |
王旭明,从二丁,罗文龙,等.固体碳源用于异养硝化去除地下水中的硝酸盐[J]. 中国科学, 2008,38(9):824-828.
|
| [14] |
Ovez B. Batch biological denitrification using Arundo donax, Glycyrrhiza glabra, and Gracilaria verrucosa as carbon source. Process Biochem. 2006,41:1289-1295.
|
| [15] |
邵留,徐祖信,金伟,等.以稻草为碳源和生物膜载体去除水中的硝酸盐[J]. 环境科学, 2009,30(5):1414-1419.
|
| [16] |
Boley A, Müller W R, Haider G. Biodegradable polymers as solid substrate and biofilm carrier for denitrification in recirculated aquaculture systems.[J]. Aquacult Eng, 2000,22:75-85.
|
| [17] |
赖才胜,谭洪新,罗国芝,等.固相反硝化反应器对含盐水体脱氮效率的预测模型[J]. 中国环境科学, 2011,31(1):32-37.
|
| [18] |
Qiu T L, Zuo Z Z, Gao J L, et al. Diaphorobacter polyhydroxybutyrativorans sp.nov., a novel poly(3-hydroxybutyrate-co-3-hydroxyvalerate)-degrading bacterium isolated from biofilms[J]. International Journal of Systematic and Evolutionary Microbiology, 2015,(65):2913-2918.
|
| [19] |
Joong K K, Kyoung J P, Kyoung S C, et al. Aerobic nitrification-denitrification by heterotrophic Bacillus strains[J]. Bioresource Technology, 2005,(96):1897-1906.
|
| [20] |
国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 4版.北京:中国环境科学出版社, 2002.
|
| [21] |
刘天琪,金若菲,周集体,等.异养硝化-好氧反硝化菌AND-42的筛选及脱氮特性[J]. 环境工程学报, 2015,9(2):989-996.
|
| [22] |
黄廷林,张丽娜,张海涵,等.一株贫营养异养硝化-好氧反硝化菌的筛选及脱氮特性[J]. 生态环境学报, 2015,28(7):1548-1552.
|
| [23] |
Chen Q, Ni J. Ammonium removal by Agrobacterium sp.LAD9 capable of heterotrophic nitrification-aerobic denitrification[J]. Journal Bioscience and Bioengineering, 2012,113(5):619-623.
|
| [24] |
徐影,仇天雷,韩梅琳,等.PCR-DGGE技术解析固体碳源表面生物膜的微生物群落结构[J]. 环境科学, 2013,34(8):3257-3263.
|
| [25] |
Aamer A S, Fariha H, Abdul H. Degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by a newly isolated Actinomadura sp. AF-555, from soil[J]. International Biodeterioration & Biodegradation, 2010,64:281-285.
|
| [26] |
Li C, Yang J S, Wang Xin, et al. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24[J]. Bioresource Technology, 2015,182:18-25.
|
| [27] |
Chu L, Wang J L. Denitrification performance and biofilm characteristics using biodegradable polymers PCL as carriers and carbon source[J]. Bioresource Technology, 2013,91:1310-1316.
|
| [28] |
Liu Y, Ai G M, Miao L L. Marinobacter strain NNA5, a newly isolated and highly efficient aerobic denitrifier with zero N2O emission[J]. Bioresource Technology, 2016,206:9-15.
|
| [29] |
Zheng M S, Li C, Liu S F, et al. Potential application of aerobic denitrifying bacterium Pseudomonas aeruginosa PCN-2 in nitrogen oxidrs (NOx) removal from flue gas[J]. Journal of Hazardous Materials, 2016,318:571-578.
|
| [30] |
何腾霞,倪九派,李振轮,等.1株Arthrobacter arilaitensis菌的耐冷异养硝化和好氧反硝化作用[J]. 环境科学, 2016,37(3):1082-1088.
|
| [31] |
孙庆花,于德爽,张培玉,等.海洋菌株y3的分离鉴定及其异养硝化-好氧反硝化特性[J]. 环境科学, 2016,37(3):1089-1097.
|
| [32] |
Chen P Z, Li J, Li Q X, et al. Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24[J]. Bioresource Technology, 2012,116:266-270.
|
| [33] |
Duan J M, Fang H D, Su B, et al. Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater[J]. Bioresource Technology, 2015,179:421-428.
|
| [34] |
Liu Y X, Hu T T, Song Y J. Heterotrophic nitrogen removal by Acinetobacter sp.Y1isolated from coke plant wastewater[J]. Journal of Bioscience and Bioengineering, 2015,120:549-554.
|
| [35] |
张颖琦,沈俊毅,徐映如,等.亚硝酸盐对人体的危害及检测方法的进展[J]. 职业与健康, 2008,31(6):851-855.
|
| [36] |
王弘宇,马放,苏俊峰,等.好氧反硝化菌株的鉴定及其反硝化特性研究[J]. 环境科学, 2008,28(7):113-120.
|
| [37] |
Sun Y L, Li A, Zhang X N, et al. Regulation of dissolved oxygen from accumulated nitrite during the heterotrophic nitrification and aerobic denitrification of Pseudomonas strtzeri T13[J]. Applied Microbiology and Biotechnology, 2015,99(7):3243-3248.
|
| [38] |
刘秀红,彭轶,马涛,等.DO浓度对生活污水硝化过程中N2O产生量的影响[J]. 环境科学, 2008,29(3):660-664.
|
|
|
|
