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| Nitrification enhancement and microbial community structure analysis for a full-scale wastewater treatment plant in winter |
| QIU Zhan |
| Shanghai Chengtou Wastewater Treatment Co., Ltd., Shanghai 201203, China |
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Abstract Two 100,000m3/d bioreactors in a full-scale wastewater treatment plant (WWTP) were operated in parallel to investigate the effect of prolonging sludge retention time (SRT) on effluent quality, nitrification rate and microbial community structure in winter. The results showed that prolonging SRT in advance greatly enhanced removal efficiencies of COD, ammonia nitrogen and total nitrogen, as well as increased the nitrification rate as thrice of the control bioreactor. Further analysis by pyrosequencing showed that the abundance and diversity of microbial populations significantly increased by prolonging SRT. Compared to the control bioreactor, the relative abundance of Nitrosomonas (ammonia oxidizing bacteria, 1.46%) and Nitrospira (nitrifying oxidizing bacteria, 0.13%) were increased by 12 and 13 times, respectively. Prolonging SRT also enriched denitrifying bacteria, including Hyphomicrobium, Thauera and Zoogloea, etc. These results indicated that prolonging SRT in advance significantly enhanced nitrifying microbial communities, which resulted in the enhancement in the nitrification and denitrification efficiency of the full-scale WWTP.
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Received: 24 February 2017
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