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Effects of two phthalate esters on zebrafish embryos |
MU Xi-yan1, LI Cheng-long2, HUANG Ying1, SHEN Gong-ming1, LI Xu-xing1, LEI Yun-lei1, HUANG Lan2, PANG Sen2, LI Ying-ren1, LI Xue-feng2, WANG Cheng-ju2 |
1. Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing 100141, China;
2. College of Sciences, China Agricultural University, Beijing 100193 |
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Abstract The effects of di (2-ethylhexyl) phthalate (DEHP) and dibutyl phthalate (DBP) on morphological indicators during zebrafish embryonic development were investigated in this study. In addition, the protein level of Nkx2.5 and LOX (two proteins that are associated with embryonic development) as well as the thyroid hormone level (triiodothyronine (T3) and tetraiodothyronine (T4)) were detected after DEHP and DBP exposure. The results indicated that the exposure of tested concentrations (10~500μg/L) of DEHP and DBP could induce a suite of abnormities on zebrafish embryos, including abnormal spontaneous movement, heart rate decrease, pericardial edema and spinal curvature, which occurred along with the decrease of Nkx2.5 level. ELISA assay results showed that the level of T3 and T4 significantly increased after exposure of 500 μg/L DEHP and DBP, which indicated that PAEs have endocrine disrupting effects on fish during early life stage. Furthermore, the LOEC of DEHP and DBP towards zebrafish embryos was 10μg/L, which was close to their detected concentrations in environmental water areas. Thus their potential risk on environmental aquatic organisms during early life stages should be concerned.
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Received: 10 February 2017
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