Pilot-scale bio-filters were constructed to removal of high-iron, manganese and ammonia nitrogen (8~10℃, TFe 6~14mg/L, Mn 0.8~1.5mg/L, NH₄⁺-N 2.0~3.0mg/L) from groundwater and the oxidation kinetics was analyzed. The results showed that Aeration and Filter process and Aeration and Double Filtration process failed due to lack of dissolved oxygen (DO). Biological Aerated Filter was the worst process due to aeration on the water flow turbulence; Biological Aerated Filter and Filtration process was successful, However, the filter rate was only 6.0m/h; Double Aeration and Double Filtration (DADF) was the best process, which was successful to removal of high-iron manganese and ammonia nitrogen from groundwater (8~10℃, TFe 17.66mg/L, Mn 1.71mg/L, NH₄⁺-N 3.37mg/L), and the maximum filter rate of the first stage and the second stage were 13.25m/h and 12.75m/h. Moreover, which also could be applied to the purification of low-temperature groundwater (5~6℃, TFe 9.72mg/L, Mn 3.29mg/L, NH₄⁺-N 3.44mg/L), and the maximum filter rate of the first stage and the second stage were 10.0m/h and 8.0m/h. The Oxidation kinetics demonstrated that, iron was oxidized and removed followed the first-order chemical oxidation kinetics, and the oxidation kinetics constant was (1.02~1.18)×10, and the removal of manganese and ammonia nitrogen followed the kinetic law of zero-order enzymatic reaction, and the oxidation kinetics constant were (0.15~0.83)×10^-1 and (0.31~1.20)×10^-1. The oxidation rate of iron was the fastest and was first removed. The order of oxidation and removal of manganese and ammonia nitrogen was greatly affected by substrate concentration.