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Comparison to the characteristics and archaea communites under different temperature stages of thermophilic, mesophilic and psychrophilic of fixed bed anaerobic reactor |
ZHAO Hong-yan1, LI Xue1, LI Nan1, LIU Hong-dou1, PIAO Ren-zhe1, XU Guang-bo1, LI Yan-ru1, WANG Wei-dong2, CUI Zong-jun3 |
1. Agricultural College, Yanbian University, Yanji 133002, China; 2. Life Sciences and Biotechnology, Heilongjiang August First Land Reclamation University, Daqing 163000, China; 3. Center of Biomass Engineering, Agricultural University, Beijing 100091, China |
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Abstract In order to investigate the change of characteristics and methanogenic microorganisms under three temperature stages. Comparison to the dynamic of methane character and archaea communites under three temperature stages of thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The result showed that the order of absolute biogas production from high to low was thermophilic (50℃), mesophilic(35℃) and psychrophilic(4℃). The order of biogas production mesophilic, psychrophilic and thermophilic was 2.84, 2.5 and 1.8L/OLR, respectively. The order of methane content psychrophilic, mesophilic and thermophilic was 74.5, 63.5 and 57.3%, respectively. The volatile organic acids content was a big influence under different temperature stage. Clone library analysis that the diversity of microbiology was significant difference within different temperature stage. Quantitative PCR analysis that the methanosaetaceae was the dominant methanogens within 50℃ and 35℃reactors, the methanomicrobiales was the dominant methanogens in the adhering sludge at 4℃. From energy consumption an economic benefit analysis that the biogas fermentation was more suitable under low temperature, and the main metabolic pathways was hydrogenotrophic methanogenesis.
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Received: 16 June 2017
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